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O74429

- VIP1_SCHPO

UniProt

O74429 - VIP1_SCHPO

Protein

Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase

Gene

asp1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Bifunctional inositol kinase that acts in concert with the IP6K kinases to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. Phosphorylates inositol hexakisphosphate (InsP6) at positions 1 or 3 to produce PP-InsP5 which is in turn phosphorylated by IP6Ks to produce (PP)2-InsP4. Alternatively, phosphorylates at position 1 or 3 PP-InsP5, produced by IP6Ks from InsP6, to produce (PP)2-InsP4 By similarity. Required for maintaining cellular integrity, normal growth and interactions with the ARP complex. Acts as a regulator of the PHO80-PHO85 cyclin/cyclin-dependent kinase (CDK) complex, thereby regulating signaling of phosphate availability By similarity. Required for the function of the cortical actin cytoskeleton, possibly by participating in correct F-actin localization and ensuring polarized growth.By similarity1 Publication

    Catalytic activityi

    ATP + 1D-myo-inositol hexakisphosphate = ADP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate.
    ATP + 1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate = ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate.
    ATP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate = ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate.
    ATP + 1D-myo-inositol hexakisphosphate = ADP + 1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei123 – 1231ATPBy similarity
    Binding sitei197 – 1971ATPBy similarity
    Binding sitei204 – 2041ATPBy similarity
    Binding sitei223 – 2231ATPBy similarity
    Binding sitei260 – 2601SubstrateBy similarity
    Binding sitei274 – 2741SubstrateBy similarity
    Binding sitei276 – 2761ATPBy similarity
    Binding sitei321 – 3211ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi248 – 2514ATPBy similarity
    Nucleotide bindingi258 – 2603ATPBy similarity
    Nucleotide bindingi333 – 3353ATPBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. diphosphoinositol-pentakisphosphate kinase activity Source: UniProtKB-EC
    4. inositol heptakisphosphate kinase activity Source: PomBase
    5. inositol hexakisphosphate 1-kinase activity Source: UniProtKB-EC
    6. inositol hexakisphosphate 3-kinase activity Source: UniProtKB-EC
    7. inositol hexakisphosphate 5-kinase activity Source: UniProtKB-EC
    8. inositol hexakisphosphate kinase activity Source: PomBase

    GO - Biological processi

    1. cortical actin cytoskeleton organization Source: PomBase
    2. establishment or maintenance of actin cytoskeleton polarity Source: PomBase
    3. inositol phosphate biosynthetic process Source: PomBase

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase (EC:2.7.4.21, EC:2.7.4.24)
    Alternative name(s):
    Cortical actin cytoskeleton protein asp1
    InsP6 and PP-IP5 kinase
    Gene namesi
    Name:asp1
    Synonyms:vip1
    ORF Names:SPCC1672.06c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome III

    Organism-specific databases

    PomBaseiSPCC1672.06c.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. cytoskeleton Source: UniProtKB-SubCell
    3. cytosol Source: PomBase
    4. nucleus Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 920920Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinasePRO_0000270923Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei19 – 191Phosphothreonine1 Publication
    Modified residuei21 – 211Phosphoserine1 Publication
    Modified residuei706 – 7061Phosphoserine1 Publication
    Modified residuei720 – 7201Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO74429.

    Interactioni

    Protein-protein interaction databases

    BioGridi275814. 89 interactions.
    MINTiMINT-4678967.
    STRINGi4896.SPCC1672.06c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO74429.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni42 – 432Substrate bindingBy similarity
    Regioni223 – 2242Substrate bindingBy similarity
    Regioni338 – 3414Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG245915.
    HOGENOMiHOG000177917.
    OMAiIFEEDVI.
    OrthoDBiEOG7673KF.
    PhylomeDBiO74429.

    Family and domain databases

    Gene3Di3.40.50.1240. 4 hits.
    InterProiIPR013651. ATP-grasp_RimK-type.
    IPR000560. His_Pase_superF_clade-2.
    IPR029033. His_PPase_superfam.
    [Graphical view]
    PfamiPF00328. His_Phos_2. 1 hit.
    PF08443. RimK. 1 hit.
    [Graphical view]
    SUPFAMiSSF53254. SSF53254. 4 hits.

    Sequencei

    Sequence statusi: Complete.

    O74429-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIQNASHLTS IDTESSTRTA SPVSSIVTPT KRNVVGICAM DAKARSKPCR    50
    NILNRIIAEG EFEAIVFGDN MILDEAVENW PACDYLICFY SSGFPLKKAE 100
    KYVELRKPFC VNDVVFQELL WDRRLVLNIL DAIRVSTPQR LICSRDGGPK 150
    INKVLEEKLR RKFGIEITEV PTPEVKMLDE DTLSVDGKII KKPYVEKPVY 200
    GEDHNIYIYF PKSVGGGGRK LFRKVANKSS DYDPDLCAPR TEGSFIYEEF 250
    MNVDNAEDVK VYTVGPHYSH AETRKSPVVD GIVRRNPHGK EIRFITNLSE 300
    EEKNMASKIS IAFEQPVCGF DLLRVSGQSY VIDVNGWSFV KDNNDYYDNA 350
    ARILKQMFHV AERHRRNRVP SVQEVLNPPP RESEAWRLKS LVGVLRHADR 400
    TPKQKFKFSF TSDPFVKLLQ GHTEEVILRN EQLNSVLAAT NLATELKCED 450
    INKLKQLRLA LETKKDLPGT KVQLKPAYSP EGKLLKLQLI IKWGGEFTHS 500
    ARYQSKDLGE QFHKDLYIMN RDCLKDVEIY TSSERRVSAS AEIFAMAFLE 550
    QETIPSDLLK VRKDLLDDSN AAKDTMDKVK KHLKSLLRVG DTARKEFTWP 600
    ENMPKPCEVM QQVVQLMKYH RAVMRENFII LGPEVEQVQS RWCCNENPAL 650
    FRERWEKLFS EFCDSEKADP SKVSELYDTL KYDALHNRQF LERIFTPYQY 700
    LKLPQSPSLI AKEPPQRTDS NGNLVGMTGA NTNHTERPLE KLYELYDLAK 750
    VLFDFVSPQE YGIEPKEKLE IGLLTSVPLL RQIIHDIKEA RDSDHASTRM 800
    YFTKESHIYT LLNCILESGL PMKLPRNQIP ELDYLTQICF ELFERTNPSG 850
    NKEFSVRITL SPGCYAQCPL DMNLDAKHCI SVSPRRSLTR HLDLQQFITK 900
    TEDLCNSVHL PKRFIPVNIN 920
    Length:920
    Mass (Da):105,680
    Last modified:November 1, 1998 - v1
    Checksum:i507872D240110C0A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329672 Genomic DNA. Translation: CAA20444.1.
    PIRiT41050.
    RefSeqiNP_587877.1. NM_001022869.2.

    Genome annotation databases

    EnsemblFungiiSPCC1672.06c.1; SPCC1672.06c.1:pep; SPCC1672.06c.
    GeneIDi2539244.
    KEGGispo:SPCC1672.06c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329672 Genomic DNA. Translation: CAA20444.1 .
    PIRi T41050.
    RefSeqi NP_587877.1. NM_001022869.2.

    3D structure databases

    ProteinModelPortali O74429.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 275814. 89 interactions.
    MINTi MINT-4678967.
    STRINGi 4896.SPCC1672.06c-1.

    Proteomic databases

    MaxQBi O74429.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPCC1672.06c.1 ; SPCC1672.06c.1:pep ; SPCC1672.06c .
    GeneIDi 2539244.
    KEGGi spo:SPCC1672.06c.

    Organism-specific databases

    PomBasei SPCC1672.06c.

    Phylogenomic databases

    eggNOGi NOG245915.
    HOGENOMi HOG000177917.
    OMAi IFEEDVI.
    OrthoDBi EOG7673KF.
    PhylomeDBi O74429.

    Miscellaneous databases

    NextBioi 20800414.
    PROi O74429.

    Family and domain databases

    Gene3Di 3.40.50.1240. 4 hits.
    InterProi IPR013651. ATP-grasp_RimK-type.
    IPR000560. His_Pase_superF_clade-2.
    IPR029033. His_PPase_superfam.
    [Graphical view ]
    Pfami PF00328. His_Phos_2. 1 hit.
    PF08443. RimK. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53254. SSF53254. 4 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "Identification and characterization of Schizosaccharomyces pombe asp1(+), a gene that interacts with mutations in the Arp2/3 complex and actin."
      Feoktistova A., McCollum D., Ohi R., Gould K.L.
      Genetics 152:895-908(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-19; SER-21; SER-706 AND SER-720, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiVIP1_SCHPO
    AccessioniPrimary (citable) accession number: O74429
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Caution

    Although related to histidine acid phosphatase proteins, it lacks the conserved active sites, suggesting that it has no phosphatase activity.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3