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O74429

- VIP1_SCHPO

UniProt

O74429 - VIP1_SCHPO

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Protein
Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase
Gene
asp1, vip1, SPCC1672.06c
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Bifunctional inositol kinase that acts in concert with the IP6K kinases to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. Phosphorylates inositol hexakisphosphate (InsP6) at positions 1 or 3 to produce PP-InsP5 which is in turn phosphorylated by IP6Ks to produce (PP)2-InsP4. Alternatively, phosphorylates at position 1 or 3 PP-InsP5, produced by IP6Ks from InsP6, to produce (PP)2-InsP4 By similarity. Required for maintaining cellular integrity, normal growth and interactions with the ARP complex. Acts as a regulator of the PHO80-PHO85 cyclin/cyclin-dependent kinase (CDK) complex, thereby regulating signaling of phosphate availability By similarity. Required for the function of the cortical actin cytoskeleton, possibly by participating in correct F-actin localization and ensuring polarized growth.1 Publication

Catalytic activityi

ATP + 1D-myo-inositol hexakisphosphate = ADP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate.
ATP + 1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate = ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate.
ATP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate = ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate.
ATP + 1D-myo-inositol hexakisphosphate = ADP + 1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei123 – 1231ATP By similarity
Binding sitei197 – 1971ATP By similarity
Binding sitei204 – 2041ATP By similarity
Binding sitei223 – 2231ATP By similarity
Binding sitei260 – 2601Substrate By similarity
Binding sitei274 – 2741Substrate By similarity
Binding sitei276 – 2761ATP By similarity
Binding sitei321 – 3211ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi248 – 2514ATP By similarity
Nucleotide bindingi258 – 2603ATP By similarity
Nucleotide bindingi333 – 3353ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. acid phosphatase activity Source: InterPro
  3. diphosphoinositol-pentakisphosphate kinase activity Source: UniProtKB-EC
  4. inositol heptakisphosphate kinase activity Source: PomBase
  5. inositol hexakisphosphate 1-kinase activity Source: UniProtKB-EC
  6. inositol hexakisphosphate 3-kinase activity Source: UniProtKB-EC
  7. inositol hexakisphosphate 5-kinase activity Source: UniProtKB-EC
  8. inositol hexakisphosphate kinase activity Source: PomBase

GO - Biological processi

  1. cortical actin cytoskeleton organization Source: PomBase
  2. establishment or maintenance of actin cytoskeleton polarity Source: PomBase
  3. inositol phosphate biosynthetic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase (EC:2.7.4.21, EC:2.7.4.24)
Alternative name(s):
Cortical actin cytoskeleton protein asp1
InsP6 and PP-IP5 kinase
Gene namesi
Name:asp1
Synonyms:vip1
ORF Names:SPCC1672.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome III

Organism-specific databases

PomBaseiSPCC1672.06c.

Subcellular locationi

Cytoplasm. Nucleus. Cytoplasmcytoskeleton 2 Publications

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytoskeleton Source: UniProtKB-SubCell
  3. cytosol Source: PomBase
  4. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 920920Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase
PRO_0000270923Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Phosphothreonine1 Publication
Modified residuei21 – 211Phosphoserine1 Publication
Modified residuei706 – 7061Phosphoserine1 Publication
Modified residuei720 – 7201Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO74429.

Interactioni

Protein-protein interaction databases

BioGridi275814. 89 interactions.
MINTiMINT-4678967.
STRINGi4896.SPCC1672.06c-1.

Structurei

3D structure databases

ProteinModelPortaliO74429.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni42 – 432Substrate binding By similarity
Regioni223 – 2242Substrate binding By similarity
Regioni338 – 3414Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG245915.
HOGENOMiHOG000177917.
OMAiIFEEDVI.
OrthoDBiEOG7673KF.
PhylomeDBiO74429.

Family and domain databases

Gene3Di3.40.50.1240. 4 hits.
InterProiIPR013651. ATP-grasp_RimK-type.
IPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
PF08443. RimK. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 4 hits.

Sequencei

Sequence statusi: Complete.

O74429-1 [UniParc]FASTAAdd to Basket

« Hide

MIQNASHLTS IDTESSTRTA SPVSSIVTPT KRNVVGICAM DAKARSKPCR    50
NILNRIIAEG EFEAIVFGDN MILDEAVENW PACDYLICFY SSGFPLKKAE 100
KYVELRKPFC VNDVVFQELL WDRRLVLNIL DAIRVSTPQR LICSRDGGPK 150
INKVLEEKLR RKFGIEITEV PTPEVKMLDE DTLSVDGKII KKPYVEKPVY 200
GEDHNIYIYF PKSVGGGGRK LFRKVANKSS DYDPDLCAPR TEGSFIYEEF 250
MNVDNAEDVK VYTVGPHYSH AETRKSPVVD GIVRRNPHGK EIRFITNLSE 300
EEKNMASKIS IAFEQPVCGF DLLRVSGQSY VIDVNGWSFV KDNNDYYDNA 350
ARILKQMFHV AERHRRNRVP SVQEVLNPPP RESEAWRLKS LVGVLRHADR 400
TPKQKFKFSF TSDPFVKLLQ GHTEEVILRN EQLNSVLAAT NLATELKCED 450
INKLKQLRLA LETKKDLPGT KVQLKPAYSP EGKLLKLQLI IKWGGEFTHS 500
ARYQSKDLGE QFHKDLYIMN RDCLKDVEIY TSSERRVSAS AEIFAMAFLE 550
QETIPSDLLK VRKDLLDDSN AAKDTMDKVK KHLKSLLRVG DTARKEFTWP 600
ENMPKPCEVM QQVVQLMKYH RAVMRENFII LGPEVEQVQS RWCCNENPAL 650
FRERWEKLFS EFCDSEKADP SKVSELYDTL KYDALHNRQF LERIFTPYQY 700
LKLPQSPSLI AKEPPQRTDS NGNLVGMTGA NTNHTERPLE KLYELYDLAK 750
VLFDFVSPQE YGIEPKEKLE IGLLTSVPLL RQIIHDIKEA RDSDHASTRM 800
YFTKESHIYT LLNCILESGL PMKLPRNQIP ELDYLTQICF ELFERTNPSG 850
NKEFSVRITL SPGCYAQCPL DMNLDAKHCI SVSPRRSLTR HLDLQQFITK 900
TEDLCNSVHL PKRFIPVNIN 920
Length:920
Mass (Da):105,680
Last modified:November 1, 1998 - v1
Checksum:i507872D240110C0A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329672 Genomic DNA. Translation: CAA20444.1.
PIRiT41050.
RefSeqiNP_587877.1. NM_001022869.2.

Genome annotation databases

EnsemblFungiiSPCC1672.06c.1; SPCC1672.06c.1:pep; SPCC1672.06c.
GeneIDi2539244.
KEGGispo:SPCC1672.06c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329672 Genomic DNA. Translation: CAA20444.1 .
PIRi T41050.
RefSeqi NP_587877.1. NM_001022869.2.

3D structure databases

ProteinModelPortali O74429.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 275814. 89 interactions.
MINTi MINT-4678967.
STRINGi 4896.SPCC1672.06c-1.

Proteomic databases

MaxQBi O74429.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPCC1672.06c.1 ; SPCC1672.06c.1:pep ; SPCC1672.06c .
GeneIDi 2539244.
KEGGi spo:SPCC1672.06c.

Organism-specific databases

PomBasei SPCC1672.06c.

Phylogenomic databases

eggNOGi NOG245915.
HOGENOMi HOG000177917.
OMAi IFEEDVI.
OrthoDBi EOG7673KF.
PhylomeDBi O74429.

Miscellaneous databases

NextBioi 20800414.
PROi O74429.

Family and domain databases

Gene3Di 3.40.50.1240. 4 hits.
InterProi IPR013651. ATP-grasp_RimK-type.
IPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view ]
Pfami PF00328. His_Phos_2. 1 hit.
PF08443. RimK. 1 hit.
[Graphical view ]
SUPFAMi SSF53254. SSF53254. 4 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Identification and characterization of Schizosaccharomyces pombe asp1(+), a gene that interacts with mutations in the Arp2/3 complex and actin."
    Feoktistova A., McCollum D., Ohi R., Gould K.L.
    Genetics 152:895-908(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-19; SER-21; SER-706 AND SER-720, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiVIP1_SCHPO
AccessioniPrimary (citable) accession number: O74429
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

Although related to histidine acid phosphatase proteins, it lacks the conserved active sites, suggesting that it has no phosphatase activity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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