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Protein

Epsin-1

Gene

ent1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to membranes enriched in phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Required for endocytosis and localization of actin.1 Publication

GO - Molecular functioni

GO - Biological processi

  • actin cortical patch assembly Source: PomBase
  • actin filament organization Source: PomBase
  • endocytosis Source: PomBase
  • positive regulation of GTPase activity Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Epsin-1
Gene namesi
Name:ent1
ORF Names:SPCC162.07
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC162.07.
PomBaseiSPCC162.07. ent1.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Membrane PROSITE-ProRule annotation1 Publication; Peripheral membrane protein 1 Publication

  • Note: Localizes in a punctate pattern. Colocalizes with F-actin.

GO - Cellular componenti

  • actin cortical patch Source: PomBase
  • membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi90 – 901G → S: Aberrant cell morphology and depolarization of F-actin. 1 Publication
Mutagenesisi107 – 1071T → A: Aberrant cell morphology and depolarization of F-actin. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 706706Epsin-1PRO_0000074523Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei216 – 2161Phosphoserine1 Publication
Modified residuei220 – 2201Phosphoserine1 Publication
Modified residuei222 – 2221Phosphoserine1 Publication
Modified residuei227 – 2271Phosphoserine1 Publication
Modified residuei259 – 2591Phosphoserine1 Publication
Modified residuei410 – 4101Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO74423.

PTM databases

iPTMnetiO74423.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi275955. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliO74423.
SMRiO74423. Positions 14-150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 146133ENTHPROSITE-ProRule annotationAdd
BLAST
Domaini230 – 24920UIM 1PROSITE-ProRule annotationAdd
BLAST
Domaini258 – 27720UIM 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi146 – 21873Arg-richAdd
BLAST
Compositional biasi278 – 699422Gln-richAdd
BLAST

Sequence similaritiesi

Belongs to the epsin family.Curated
Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation
Contains 2 UIM (ubiquitin-interacting motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

InParanoidiO74423.
KOiK12471.
OMAiGFVQPQA.
OrthoDBiEOG7KM63T.
PhylomeDBiO74423.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR013182. DUF1720.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
IPR003903. UIM_dom.
[Graphical view]
PfamiPF08226. DUF1720. 4 hits.
PF01417. ENTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
SM00726. UIM. 2 hits.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
PS50330. UIM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74423-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFSALAYNL LAKNFSKGYT DTQIKVRNAT TNDSWGPSGT AMAEIAELTY
60 70 80 90 100
DQNEMLEVMD IIDRRLNDKG KNWRHVFKSL SLLEYCLHNG SENVVRWAKD
110 120 130 140 150
NIYIITTLRE FVYVDDNGHD QGQNVRTKAK EITSLLEDEH ALKEARGDSR
160 170 180 190 200
ERDRDRDRTR SSRFDDDDDD RAPYEESRLS RAPSRASRYD DDDRDHRSRR
210 220 230 240 250
RSRSRRPGRS RSRRRSRRPS PSAEHNSAEE NDPELQRVIE ESKRQAEEDA
260 270 280 290 300
KRRNMANDSE AELQKAIQLS KEEDEARQRH QREREQQEQA FMGNQQNAYQ
310 320 330 340 350
PVDFFGNPVQ PQPTGFLQQQ PTGFIRPQNT GFVQPQYTGF VQPQHTGFVQ
360 370 380 390 400
PQATGFMQPQ RTGFVQPQAT GFVQPQATGF VQPQATGFMQ PQRTGFVQPQ
410 420 430 440 450
ATGFMQPQRT GFVQPQATGF MQPQRTGFVQ PQATGFIQPQ RTGFVQPQQN
460 470 480 490 500
GFFNPQPTGY MQPQRTGMMQ PQRTGFSQPF ESNNPFPVMQ PQRTGFGQTP
510 520 530 540 550
NAPMMAPNHT GYVHPQPTGL QRQTTGYTGN NNPYSRPLQS QSTGILQQQQ
560 570 580 590 600
QQSAPRLEPT KTGSNNPFAQ FSNLPSQSTA PATKPMKPVR TGDDRFSNIA
610 620 630 640 650
QAISTGNPMG TDSFGNIGLT RVPTQHTGSK FTNSAGQTIQ AQATGNTHNP
660 670 680 690 700
FQSQQATGYY KQPMQQQQNM QQPYYNQQNY NYQNQQPMQG MQQQSMQPQV

GSLIDL
Length:706
Mass (Da):80,174
Last modified:November 1, 1998 - v1
Checksum:i099F1E6CFEE549FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA19587.1.
PIRiT41024.
RefSeqiNP_588237.1. NM_001023227.2.

Genome annotation databases

GeneIDi2539390.
KEGGispo:SPCC162.07.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA19587.1.
PIRiT41024.
RefSeqiNP_588237.1. NM_001023227.2.

3D structure databases

ProteinModelPortaliO74423.
SMRiO74423. Positions 14-150.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275955. 3 interactions.

PTM databases

iPTMnetiO74423.

Proteomic databases

MaxQBiO74423.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2539390.
KEGGispo:SPCC162.07.

Organism-specific databases

EuPathDBiFungiDB:SPCC162.07.
PomBaseiSPCC162.07. ent1.

Phylogenomic databases

InParanoidiO74423.
KOiK12471.
OMAiGFVQPQA.
OrthoDBiEOG7KM63T.
PhylomeDBiO74423.

Miscellaneous databases

NextBioi20800554.
PROiO74423.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR013182. DUF1720.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
IPR003903. UIM_dom.
[Graphical view]
PfamiPF08226. DUF1720. 4 hits.
PF01417. ENTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
SM00726. UIM. 2 hits.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
PS50330. UIM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Fission yeast epsin, Ent1p is required for endocytosis and involved in actin organization."
    Sakamoto C., Kawamoto C., Takeuchi K., Miyamoto I., Shuntoh H.
    Kobe J. Med. Sci. 50:47-57(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-90 AND THR-107.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216; SER-220; SER-222; SER-227; SER-259 AND THR-410, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiENT1_SCHPO
AccessioniPrimary (citable) accession number: O74423
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: November 1, 1998
Last modified: May 11, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.