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Protein

Epsin-1

Gene

ent1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to membranes enriched in phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Required for endocytosis and localization of actin.1 Publication

GO - Molecular functioni

  • clathrin binding Source: PomBase
  • phosphatidylinositol-4,5-bisphosphate binding Source: PomBase

GO - Biological processi

  • actin cortical patch assembly Source: PomBase
  • actin filament organization Source: PomBase
  • endocytosis Source: PomBase
  • positive regulation of GTPase activity Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

ReactomeiR-SPO-8856825. Cargo recognition for clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Epsin-1
Gene namesi
Name:ent1
ORF Names:SPCC162.07
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC162.07.
PomBaseiSPCC162.07. ent1.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Membrane PROSITE-ProRule annotation1 Publication; Peripheral membrane protein 1 Publication

  • Note: Localizes in a punctate pattern. Colocalizes with F-actin.

GO - Cellular componenti

  • actin cortical patch Source: PomBase
  • membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi86G → S: Aberrant cell morphology and depolarization of F-actin. 1 Publication1
Mutagenesisi103T → A: Aberrant cell morphology and depolarization of F-actin. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745231 – 702Epsin-1Add BLAST702

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei212Phosphoserine1 Publication1
Modified residuei216Phosphoserine1 Publication1
Modified residuei218Phosphoserine1 Publication1
Modified residuei223Phosphoserine1 Publication1
Modified residuei255Phosphoserine1 Publication1
Modified residuei406Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO74423.
PRIDEiO74423.

PTM databases

iPTMnetiO74423.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi275955. 3 interactors.

Structurei

3D structure databases

ProteinModelPortaliO74423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 142ENTHPROSITE-ProRule annotationAdd BLAST133
Domaini226 – 245UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini254 – 273UIM 2PROSITE-ProRule annotationAdd BLAST20

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi142 – 214Arg-richPROSITE-ProRule annotationAdd BLAST73
Compositional biasi274 – 695Gln-richPROSITE-ProRule annotationAdd BLAST422

Sequence similaritiesi

Belongs to the epsin family.Curated
Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation
Contains 2 UIM (ubiquitin-interacting motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

InParanoidiO74423.
KOiK12471.
OMAiQATGFVQ.
OrthoDBiEOG092C4BNK.
PhylomeDBiO74423.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR013182. DUF1720.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
IPR003903. UIM_dom.
[Graphical view]
PfamiPF08226. DUF1720. 3 hits.
PF01417. ENTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
SM00726. UIM. 2 hits.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
PS50330. UIM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74423-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAAVRSVKN FSKGYTDTQI KVRNATTNDS WGPSGTAMAE IAELTYDQNE
60 70 80 90 100
MLEVMDIIDR RLNDKGKNWR HVFKSLSLLE YCLHNGSENV VRWAKDNIYI
110 120 130 140 150
ITTLREFVYV DDNGHDQGQN VRTKAKEITS LLEDEHALKE ARGDSRERDR
160 170 180 190 200
DRDRTRSSRF DDDDDDRAPY EESRLSRAPS RASRYDDDDR DHRSRRRSRS
210 220 230 240 250
RRPGRSRSRR RSRRPSPSAE HNSAEENDPE LQRVIEESKR QAEEDAKRRN
260 270 280 290 300
MANDSEAELQ KAIQLSKEED EARQRHQRER EQQEQAFMGN QQNAYQPVDF
310 320 330 340 350
FGNPVQPQPT GFLQQQPTGF IRPQNTGFVQ PQYTGFVQPQ HTGFVQPQAT
360 370 380 390 400
GFMQPQRTGF VQPQATGFVQ PQATGFVQPQ ATGFMQPQRT GFVQPQATGF
410 420 430 440 450
MQPQRTGFVQ PQATGFMQPQ RTGFVQPQAT GFIQPQRTGF VQPQQNGFFN
460 470 480 490 500
PQPTGYMQPQ RTGMMQPQRT GFSQPFESNN PFPVMQPQRT GFGQTPNAPM
510 520 530 540 550
MAPNHTGYVH PQPTGLQRQT TGYTGNNNPY SRPLQSQSTG ILQQQQQQSA
560 570 580 590 600
PRLEPTKTGS NNPFAQFSNL PSQSTAPATK PMKPVRTGDD RFSNIAQAIS
610 620 630 640 650
TGNPMGTDSF GNIGLTRVPT QHTGSKFTNS AGQTIQAQAT GNTHNPFQSQ
660 670 680 690 700
QATGYYKQPM QQQQNMQQPY YNQQNYNYQN QQPMQGMQQQ SMQPQVGSLI

DL
Length:702
Mass (Da):79,751
Last modified:October 5, 2016 - v2
Checksum:i6B3ED8F0BD3400AA
GO

Sequence cautioni

The sequence CAA19587 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA19587.1. Sequence problems.
PIRiT41024.

Genome annotation databases

KEGGispo:SPCC162.07.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA19587.1. Sequence problems.
PIRiT41024.

3D structure databases

ProteinModelPortaliO74423.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275955. 3 interactors.

PTM databases

iPTMnetiO74423.

Proteomic databases

MaxQBiO74423.
PRIDEiO74423.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGispo:SPCC162.07.

Organism-specific databases

EuPathDBiFungiDB:SPCC162.07.
PomBaseiSPCC162.07. ent1.

Phylogenomic databases

InParanoidiO74423.
KOiK12471.
OMAiQATGFVQ.
OrthoDBiEOG092C4BNK.
PhylomeDBiO74423.

Enzyme and pathway databases

ReactomeiR-SPO-8856825. Cargo recognition for clathrin-mediated endocytosis.

Miscellaneous databases

PROiO74423.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR013182. DUF1720.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
IPR003903. UIM_dom.
[Graphical view]
PfamiPF08226. DUF1720. 3 hits.
PF01417. ENTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
SM00726. UIM. 2 hits.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
PS50330. UIM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiENT1_SCHPO
AccessioniPrimary (citable) accession number: O74423
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 5, 2016
Last modified: November 30, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.