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Protein

Uricase

Gene

SPCC1223.09

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.1 Publication

Catalytic activityi

Urate + O2 + H2O = 5-hydroxyisourate + H2O2.1 Publication

Pathwayi: urate degradation

This protein is involved in step 1 of the subpathway that synthesizes (S)-allantoin from urate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Uricase (SPCC1223.09)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway urate degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-allantoin from urate, the pathway urate degradation and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei180 – 1801Charge relay systemBy similarity
Binding sitei180 – 1801SubstrateBy similarity
Active sitei230 – 2301Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Purine metabolism

Enzyme and pathway databases

UniPathwayiUPA00394; UER00650.

Names & Taxonomyi

Protein namesi
Recommended name:
Uricase (EC:1.7.3.3)
Alternative name(s):
Urate oxidase
Gene namesi
ORF Names:SPCC1223.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC1223.09.
PomBaseiSPCC1223.09.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nucleus Source: PomBase
  • peroxisome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296UricasePRO_0000165998Add
BLAST

Proteomic databases

MaxQBiO74409.

Interactioni

Protein-protein interaction databases

BioGridi275531. 1 interaction.
MINTiMINT-4678818.

Structurei

3D structure databases

ProteinModelPortaliO74409.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni61 – 622Substrate bindingBy similarity
Regioni229 – 2302Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the uricase family.Curated

Phylogenomic databases

HOGENOMiHOG000250659.
InParanoidiO74409.
KOiK00365.
OMAiKDRCFAT.
OrthoDBiEOG75MW65.
PhylomeDBiO74409.

Family and domain databases

InterProiIPR002042. Uricase.
IPR019842. Uricase_CS.
[Graphical view]
PfamiPF01014. Uricase. 2 hits.
[Graphical view]
PIRSFiPIRSF000241. Urate_oxidase. 1 hit.
PRINTSiPR00093. URICASE.
TIGRFAMsiTIGR03383. urate_oxi. 1 hit.
PROSITEiPS00366. URICASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74409-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSETTYVKQC AYGKTLVRFM KKDICPKTKT HTVYEMDVQS LLTGELEESY
60 70 80 90 100
TKADNSIVVP TDTQKNTIYV FAKNNDVSVP EVFAAKLAKH FVDKYKHIHG
110 120 130 140 150
AALDITITPW TRMEVQGKPH SHSFIRNPGE TRKTHVVFSE GKGFDVVSSL
160 170 180 190 200
KDVLVLKSTG SGFTNFHKCE FTTLPEVTDR IFSTSIDCNY TFKHFDTFEE
210 220 230 240 250
LAGFDFNSIY EKVKEITLET FALDDSESVQ ATMYKMADTI INTYPAINEV
260 270 280 290
YYALPNKHYF EINLAPFNID NLGSNCSLYQ PQAYPSGYIT CTVARK
Length:296
Mass (Da):33,610
Last modified:November 1, 1998 - v1
Checksum:iC7C8DF71A7CD1EDF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA20878.1.
PIRiT40869.
RefSeqiNP_588354.1. NM_001023345.2.

Genome annotation databases

EnsemblFungiiSPCC1223.09.1; SPCC1223.09.1:pep; SPCC1223.09.
GeneIDi2538957.
KEGGispo:SPCC1223.09.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA20878.1.
PIRiT40869.
RefSeqiNP_588354.1. NM_001023345.2.

3D structure databases

ProteinModelPortaliO74409.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275531. 1 interaction.
MINTiMINT-4678818.

Proteomic databases

MaxQBiO74409.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC1223.09.1; SPCC1223.09.1:pep; SPCC1223.09.
GeneIDi2538957.
KEGGispo:SPCC1223.09.

Organism-specific databases

EuPathDBiFungiDB:SPCC1223.09.
PomBaseiSPCC1223.09.

Phylogenomic databases

HOGENOMiHOG000250659.
InParanoidiO74409.
KOiK00365.
OMAiKDRCFAT.
OrthoDBiEOG75MW65.
PhylomeDBiO74409.

Enzyme and pathway databases

UniPathwayiUPA00394; UER00650.

Miscellaneous databases

PROiO74409.

Family and domain databases

InterProiIPR002042. Uricase.
IPR019842. Uricase_CS.
[Graphical view]
PfamiPF01014. Uricase. 2 hits.
[Graphical view]
PIRSFiPIRSF000241. Urate_oxidase. 1 hit.
PRINTSiPR00093. URICASE.
TIGRFAMsiTIGR03383. urate_oxi. 1 hit.
PROSITEiPS00366. URICASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "The all2 gene is required for the induction of the purine deamination pathway in Schizosaccharomyces pombe."
    Fluri R., Kinghorn J.R.
    J. Gen. Microbiol. 131:527-532(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, FUNCTION.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiURIC_SCHPO
AccessioniPrimary (citable) accession number: O74409
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: June 8, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.