O74409 (URIC_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uricase
EC=1.7.3.3

Alternative name(s):
Urate oxidase

Gene names

ORF Names:

SPCC1223.09

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin. Ref.2
Catalytic activity	Urate + O2 + H2O = 5-hydroxyisourate + H2O2. Ref.2
Pathway	Purine metabolism; urate degradation; (S)-allantoin from urate: step 1/3.
Subcellular location	Peroxisome By similarity. Cytoplasm. Nucleus Ref.3.
Sequence similarities	Belongs to the uricase family.

Ontologies

Keywords
Biological process	Purine metabolism
Cellular component	Cytoplasm Nucleus Peroxisome
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine base metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe nucleus Inferred from direct assay. Source: GeneDB_Spombe peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	urate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Uricase		PRO_0000165998
Regions
	Region	61 – 62	2	Substrate binding By similarity
	Region	229 – 230	2	Substrate binding By similarity
Sites
	Active site	180	1	Charge relay system By similarity
	Active site	230	1	Charge relay system By similarity
	Binding site	180	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O74409 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: C7C8DF71A7CD1EDF	FASTA	296	33,610
	10 20 30 40 50 60 MSETTYVKQC AYGKTLVRFM KKDICPKTKT HTVYEMDVQS LLTGELEESY TKADNSIVVP 70 80 90 100 110 120 TDTQKNTIYV FAKNNDVSVP EVFAAKLAKH FVDKYKHIHG AALDITITPW TRMEVQGKPH 130 140 150 160 170 180 SHSFIRNPGE TRKTHVVFSE GKGFDVVSSL KDVLVLKSTG SGFTNFHKCE FTTLPEVTDR 190 200 210 220 230 240 IFSTSIDCNY TFKHFDTFEE LAGFDFNSIY EKVKEITLET FALDDSESVQ ATMYKMADTI 250 260 270 280 290 INTYPAINEV YYALPNKHYF EINLAPFNID NLGSNCSLYQ PQAYPSGYIT CTVARK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"The all2 gene is required for the induction of the purine deamination pathway in Schizosaccharomyces pombe." Fluri R., Kinghorn J.R. J. Gen. Microbiol. 131:527-532(1985) [PubMed: 4020341] [Abstract] Cited for: CATALYTIC ACTIVITY, FUNCTION.
[3]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329672 Genomic DNA. Translation: CAA20878.1.
PIR	T40869.
RefSeq	NP_588354.1. NM_001023345.1.
3D structure databases
ProteinModelPortal	O74409.
ModBase	Search...
Protein-protein interaction databases
STRING	O74409.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPCC1223.09.1; SPCC1223.09.1:pep; SPCC1223.09.
GeneID	2538957.
KEGG	spo:SPCC1223.09.
NMPDR	fig|4896.1.peg.692.
Organism-specific databases
GeneDB_Spombe	SPCC1223.09.
Phylogenomic databases
eggNOG	fuNOG06507.
GeneTree	EFGT00050000004784.
HOGENOM	HBG558657.
OMA	HDHSFVR.
OrthoDB	EOG4H1F58.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000274-MONOMER.
Gene expression databases
ArrayExpress	O74409.
Family and domain databases
InterPro	IPR002042. Uricase. IPR019842. Uricase_CS. [Graphical view]
Gene3D	G3DSA:3.10.270.10. Uricase. 1 hit.
KO	K00365.
PANTHER	PTHR10395:SF1. Uricase. 1 hit.
Pfam	PF01014. Uricase. 2 hits. [Graphical view]
PIRSF	PIRSF000241. Urate_oxidase. 1 hit.
PRINTS	PR00093. URICASE.
TIGRFAMs	TIGR03383. Urate_oxi. 1 hit.
PROSITE	PS00366. URICASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URIC_SCHPO

Accession

Primary (citable) accession number: O74409

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1998
Last modified:	November 16, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program