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O74407 (SYDC_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aspartate--tRNA ligase, cytoplasmic
EC=6.1.1.12
Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS
Gene names
Name:dps1
ORF Names:SPCC1223.07c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length580 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm Ref.2.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processaspartyl-tRNA aminoacylation

Inferred by curator. Source: GeneDB_Spombe

   Cellular componentcytosol

Inferred from direct assay. Source: GeneDB_Spombe

   Molecular functionATP binding

Inferred by curator. Source: GeneDB_Spombe

aspartate-tRNA ligase activity

Inferred from sequence or structural similarity. Source: GeneDB_Spombe

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 580580Aspartate--tRNA ligase, cytoplasmic
PRO_0000315951

Amino acid modifications

Modified residue2821Phosphoserine Ref.3
Modified residue3071Phosphoserine Ref.3

Sequences

Sequence LengthMass (Da)Tools
O74407 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 432FCC074D558A4C

FASTA58066,671
        10         20         30         40         50         60 
MSEMEKQVEN LSLEAKNEKP KEVILGEDGK PLSKKALKKL EKEREKEQKR KEREAREAEE 

        70         80         90        100        110        120 
KKKREANEID YSAGKYGDLP LNRSTARPGR TYTQISDISA KNDGQTVLLR ARVYTSRLQG 

       130        140        150        160        170        180 
NKMCFFSLRQ KYDTIQALAV VNKDTISKQM VKWCGSISLE SIVLVEGIVK KSPEIIKSAT 

       190        200        210        220        230        240 
VQDAEIHISS IYVISPIKKN LPFLVEDAGR SEEQIRESEE NAAEGDSKFV RVNLDTRLDN 

       250        260        270        280        290        300 
RVLDLRTPTN QAIFDIQAGI CQAFREFLLS NSFNEIHTPK MSGASSEGGS NVFKIQYFKT 

       310        320        330        340        350        360 
DGFLSQSPQL YKQMLIAADR ERVFEIGPVF RAEDSNTYRH MTEFTGLDLE MAFNEHYHEV 

       370        380        390        400        410        420 
MEFIEKLFLY IFKTIREKYA KQVAVVRQQY PSEDFILPDA DRIRFHFKDA VKLLKEAGYR 

       430        440        450        460        470        480 
KQLVPGQKVP EDEEFHYCED PEFDDFSTPE ERALGQIVRE KYHTDFYVID KYPSSVRPFY 

       490        500        510        520        530        540 
TMPDPEDPRY SNSYDFFMKG QEIMSGAQRI HDPELLVERM KALGVSPDVG LQQYIDAFAI 

       550        560        570        580 
GCPPHAGGGI GLERVVMFYL NLPNIRLASS FPRDPKRLLP

« Hide

References

[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed: 11859360] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[3]"Phosphoproteome analysis of fission yeast."
Wilson-Grady J.T., Villen J., Gygi S.P.
J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-282 AND SER-307, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU329672 Genomic DNA. Translation: CAA20876.1.
PIRT40867.
RefSeqNP_588352.1. NM_001023343.1.

3D structure databases

HSSPHSSP built from PDB template 1ASZ based on UniProtKB P04802.
ProteinModelPortalO74407.
SMRO74407. Positions 70-580.
ModBaseSearch...

Protein-protein interaction databases

STRINGO74407.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPCC1223.07c.1; SPCC1223.07c.1:pep; SPCC1223.07c.
GeneID2539278.
GenomeReviewsGene locus dps1 in contig CU329672_GR.
KEGGspo:SPCC1223.07c.
NMPDRfig|4896.1.peg.690.

Organism-specific databases

GeneDB_SpombeSPCC1223.07c.

Phylogenomic databases

eggNOGfuNOG06269.
GeneTreeEFGT00050000003411.
HOGENOMHBG745843.
OMARNPKQSN.
OrthoDBEOG4VHPFX.

Gene expression databases

ArrayExpressO74407.

Family and domain databases

InterProIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004523. Asp-tRNA-synth_IIb_arc/euk.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR004365. NA-bd_OB_tRNA-helicase.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK01876.
PANTHERPTHR22594. aa-tRNA-synt_II. 1 hit.
PTHR22594:SF10. PTHR22594:SF10. 1 hit.
PfamPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti. 1 hit.
[Graphical view]
PRINTSPR01042. TRNASYNTHASP.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMsTIGR00458. AspS_arch. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
PS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYDC_SCHPO
AccessionPrimary (citable) accession number: O74407
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 1, 1998
Last modified: January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents