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Protein

Probable phosphoserine phosphatase

Gene

SPBC3H7.07c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: L-serine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-serine from 3-phospho-D-glycerate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Putative D-3-phosphoglycerate dehydrogenase (SPCC364.07)
  2. Putative phosphoserine aminotransferase (SPAC1F12.07)
  3. Probable phosphoserine phosphatase (SPBC3H7.07c)
This subpathway is part of the pathway L-serine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-serine from 3-phospho-D-glycerate, the pathway L-serine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei82 – 821NucleophileBy similarity
Metal bindingi82 – 821MagnesiumBy similarity
Active sitei84 – 841Proton donorBy similarity
Metal bindingi84 – 841Magnesium; via carbonyl oxygenBy similarity
Binding sitei91 – 911SubstrateBy similarity
Binding sitei127 – 1271SubstrateBy similarity
Binding sitei217 – 2171SubstrateBy similarity
Metal bindingi240 – 2401MagnesiumBy similarity
Binding sitei243 – 2431SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Amino-acid biosynthesis, Serine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-SPO-977347. Serine biosynthesis.
UniPathwayiUPA00135; UER00198.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phosphoserine phosphatase (EC:3.1.3.3)
Short name:
PSP
Short name:
PSPase
Alternative name(s):
O-phosphoserine phosphohydrolase
Gene namesi
ORF Names:SPBC3H7.07c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC3H7.07c.
PomBaseiSPBC3H7.07c.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Probable phosphoserine phosphatasePRO_0000156884Add
BLAST

Proteomic databases

MaxQBiO74382.

Interactioni

Protein-protein interaction databases

BioGridi277484. 69 interactions.

Structurei

3D structure databases

ProteinModelPortaliO74382.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni170 – 1712Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the SerB family.Curated

Phylogenomic databases

HOGENOMiHOG000231114.
InParanoidiO74382.
KOiK01079.
OMAiCINEPDL.
OrthoDBiEOG741ZDJ.
PhylomeDBiO74382.

Family and domain databases

Gene3Di1.10.150.210. 1 hit.
3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006383. HAD-SF_hydro_IB_PSP-like.
IPR023190. Pser_Pase_dom_2.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01488. HAD-SF-IB. 1 hit.

Sequencei

Sequence statusi: Complete.

O74382-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNAAIVVSS NRPNVLEQIS SLFRDGKTRS LGEQWTLVSG QLKGTFEDAK
60 70 80 90 100
DACNRISATE NVDCNCLSEA TFSTKKKLVV FDMDSTLIQQ ECIDELAAEA
110 120 130 140 150
GIQKEVATIT SLAMNGEIDF QESLRRRVSL LQGLSVDVIN KVIGKITFTP
160 170 180 190 200
GAKQLCHCLK QMGATLVVAS GGFVPMAEYV KGQLDLDYAY ANVLEFSDDG
210 220 230 240 250
KFLTGKVQGA IVDGQRKASI LREKREELGL NKLETMAVGD GANDLVMMAE
260 270 280 290
SGLGIAFKAK PKVQLLADSK INLPSLQNAL YLLGIDEQQQ KKLLENKN
Length:298
Mass (Da):32,411
Last modified:November 1, 1998 - v1
Checksum:i4BCBCE73E9013CA5
GO

Sequence cautioni

The sequence BAA13922.1 differs from that shown. Reason: Frameshift at position 267. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89261 mRNA. Translation: BAA13922.1. Frameshift.
CU329671 Genomic DNA. Translation: CAA20303.1.
PIRiT40408.
T43186.
RefSeqiNP_595768.1. NM_001021669.2.

Genome annotation databases

EnsemblFungiiSPBC3H7.07c.1; SPBC3H7.07c.1:pep; SPBC3H7.07c.
GeneIDi2540968.
KEGGispo:SPBC3H7.07c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89261 mRNA. Translation: BAA13922.1. Frameshift.
CU329671 Genomic DNA. Translation: CAA20303.1.
PIRiT40408.
T43186.
RefSeqiNP_595768.1. NM_001021669.2.

3D structure databases

ProteinModelPortaliO74382.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277484. 69 interactions.

Proteomic databases

MaxQBiO74382.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC3H7.07c.1; SPBC3H7.07c.1:pep; SPBC3H7.07c.
GeneIDi2540968.
KEGGispo:SPBC3H7.07c.

Organism-specific databases

EuPathDBiFungiDB:SPBC3H7.07c.
PomBaseiSPBC3H7.07c.

Phylogenomic databases

HOGENOMiHOG000231114.
InParanoidiO74382.
KOiK01079.
OMAiCINEPDL.
OrthoDBiEOG741ZDJ.
PhylomeDBiO74382.

Enzyme and pathway databases

UniPathwayiUPA00135; UER00198.
ReactomeiR-SPO-977347. Serine biosynthesis.

Miscellaneous databases

PROiO74382.

Family and domain databases

Gene3Di1.10.150.210. 1 hit.
3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006383. HAD-SF_hydro_IB_PSP-like.
IPR023190. Pser_Pase_dom_2.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01488. HAD-SF-IB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
    Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
    DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: PR745.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiSERB_SCHPO
AccessioniPrimary (citable) accession number: O74382
Secondary accession number(s): P78910
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: November 1, 1998
Last modified: June 8, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.