Reviewed, UniProtKB/Swiss-Prot O74369 (NSMA_SCHPO)

Names and origin

Protein names

Recommended name:
    Putative neutral sphingomyelinase
    EC=3.1.4.12

Gene names

ORF Names:

SPBC32F12.01c, SPBC685.10c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Sphingomyelin + H2O = N-acylsphingosine + choline phosphate.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the neutral sphingomyelinase family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum Inferred from direct assay. Source: GeneDB_SPombe integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW sphingomyelin phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 424	424	Putative neutral sphingomyelinase		PRO_0000075691
Regions
	Transmembrane	335 – 357	23	Potential
	Transmembrane	364 – 386	23	Potential
Sites
	Active site	289	1	Proton acceptor By similarity
	Metal binding	49	1	Magnesium By similarity
	Site	181	1	Important for substrate recognition

Sequences

	Sequence		Length	Mass (Da)
	O74369-1 [UniParc]. Last modified June 1, 2000. Version 2. Checksum: 8C5DC4C3FEA4550F	FASTA	424	48,345
	10 20 30 40 50 60 MSVEKAPELR VLSFNCWGLR FVSKYRTERL KAVGEKLAKC DYDIVLLQEV WSIYDFQEIR 70 80 90 100 110 120 NLVSCNLVYS RFFHSAAMGA GLAMFSKFPI IESSMNKYPL NGRPQAFWRG DWYVGKGVAT 130 140 150 160 170 180 ASLQHPSGRI ISLFNTHLHA PYGKGADTYL CHRLSQAWYI SKLLRAAVQR GHIVIAAGDF 190 200 210 220 230 240 NIQPLSVPHE IITSYGLVND AWLSVYPDQV EHPPNRFSMN DKELVEIAGT TCDSRLNTWR 250 260 270 280 290 300 ENISSKDMDD FVAKRLDYVF HSPSTCEAKN AKVVFLERVP KLDCSYSDHF AIETVLSIKL 310 320 330 340 350 360 QPIPVQETRV SYSIIDDTLG ITYQYMARER LHMRLRIAHL LISIPLIIGV HVAIAWCDPA 370 380 390 400 410 420 WLKVIILFFT VMLTIAAVVN GFCIGLLFGR WEFNGLLEFV AELKEQKLLC KQYLVDHPLP FAKS

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAB39367.1.
PIR	T40641.
RefSeq	NP_596144.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O74369.
Genome annotation databases
GeneID	2541113.
KEGG	spo:SPBC32F12.01c.
NMPDR	fig|4896.1.peg.2010.
Organism-specific databases
GeneDB_Spombe	SPBC32F12.01c.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-004885-MON.
BRENDA	3.1.4.12. 653.
Gene expression databases
ArrayExpress	O74369.
Family and domain databases
InterPro	IPR005135. Endo/exonuclease/phosphatase. [Graphical view]
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NSMA_SCHPO

Accession

Primary (citable) accession number: O74369
Secondary accession number(s): Q9Y7M0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 11, 2002
Last sequence update:	June 1, 2000
Last modified:	October 13, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)