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O74327 (AVT5_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar amino acid transporter 5
Gene names
Name:avt5
Synonyms:avt8
ORF Names:SPBC1685.07c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length420 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Vacuolar amino acid transporter involved in the vacuolar uptake of histidine, glutamate, tyrosine, arginine, lysine, and serine. Required for sporulation. Ref.2 Ref.3

Subcellular location

Vacuole membrane; Multi-pass membrane protein Ref.3.

Sequence similarities

Belongs to the amino acid/polyamine transporter 2 family.

Ontologies

Keywords
   Biological processAmino-acid transport
Sporulation
Transport
   Cellular componentMembrane
Vacuole
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-arginine transmembrane import into vacuole

Inferred from mutant phenotype Ref.3. Source: PomBase

L-glutamate import involved in cellular response to nitrogen starvation

Inferred from mutant phenotype Ref.3. Source: PomBase

L-glutamate transmembrane import into vacuole

Inferred from mutant phenotype Ref.3. Source: PomBase

L-histidine transmembrane import into vacuole

Inferred from mutant phenotype Ref.3. Source: PomBase

L-lysine import involved in cellular response to nitrogen starvation

Inferred from mutant phenotype Ref.3. Source: PomBase

L-lysine transmembrane import into vacuole

Inferred from mutant phenotype Ref.3. Source: PomBase

L-serine transmembrane import into vacuole

Inferred from mutant phenotype Ref.3. Source: PomBase

L-tyrosine transmembrane import into vacuole

Inferred from mutant phenotype Ref.3. Source: PomBase

amino acid transmembrane import into vacuole

Inferred from mutant phenotype Ref.3. Source: PomBase

cellular response to nitrogen starvation

Inferred from mutant phenotype Ref.2. Source: PomBase

lithium ion import

Inferred from mutant phenotype PubMed 20699567. Source: PomBase

sporulation resulting in formation of a cellular spore

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentfungal-type vacuole membrane

Inferred from direct assay Ref.3. Source: PomBase

integral component of fungal-type vacuolar membrane

Inferred by curator. Source: PomBase

   Molecular_functionL-arginine transmembrane transporter activity

Inferred from mutant phenotype Ref.3. Source: PomBase

L-glutamate transmembrane transporter activity

Inferred from mutant phenotype Ref.3. Source: PomBase

L-histidine transmembrane transporter activity

Inferred from mutant phenotype Ref.3. Source: PomBase

L-lysine transmembrane transporter activity

Inferred from mutant phenotype Ref.3. Source: PomBase

L-serine transmembrane transporter activity

Inferred from mutant phenotype Ref.3. Source: PomBase

L-tyrosine transmembrane transporter activity

Inferred from mutant phenotype Ref.3. Source: PomBase

arginine transmembrane transporter activity

Inferred from mutant phenotype Ref.3. Source: PomBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 420420Vacuolar amino acid transporter 5
PRO_0000316193

Regions

Topological domain1 – 1919Cytoplasmic Potential
Transmembrane20 – 4021Helical; Potential
Topological domain41 – 499Vacuolar Potential
Transmembrane50 – 7021Helical; Potential
Topological domain71 – 9626Cytoplasmic Potential
Transmembrane97 – 11721Helical; Potential
Topological domain118 – 14124Vacuolar Potential
Transmembrane142 – 16221Helical; Potential
Topological domain163 – 1664Cytoplasmic Potential
Transmembrane167 – 18721Helical; Potential
Topological domain188 – 1958Vacuolar Potential
Transmembrane196 – 21621Helical; Potential
Topological domain217 – 24024Cytoplasmic Potential
Transmembrane241 – 26121Helical; Potential
Topological domain262 – 27817Vacuolar Potential
Transmembrane279 – 29921Helical; Potential
Topological domain300 – 32627Cytoplasmic Potential
Transmembrane327 – 34721Helical; Potential
Topological domain348 – 3492Vacuolar Potential
Transmembrane350 – 37021Helical; Potential
Topological domain371 – 39424Cytoplasmic Potential
Transmembrane395 – 41521Helical; Potential
Topological domain416 – 4205Vacuolar Potential

Sequences

Sequence LengthMass (Da)Tools
O74327 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: D76B8834CE66E170

FASTA42045,462
        10         20         30         40         50         60 
MSGYSPLSSG PADVHIGKAG FFSSVINLAN TILGAGILSL PNAFTKTGLL FGCLTIVFSA 

        70         80         90        100        110        120 
FASFLGLYFV SQCAARLPRG KASFAAVAKH TFPSLAVVFD ASIAVKCFGV AVSYLVIVGD 

       130        140        150        160        170        180 
LMPQIAPSLG LSSPMFLRRQ TWIVFALFVL TPLSFLKRLD SLRHTSVISL IALCYLVFIV 

       190        200        210        220        230        240 
LYHFIIGDTV KGEIRYFVPE SGFGYLSVLP VFVFGFTCHQ NAFSVINEVR NFSQGFVNFT 

       250        260        270        280        290        300 
MFTAIISSTL LYLLVAITGY LSFGSLASGN IIAMYDNTSI WIIGGKLAIV VLVLFSYPLQ 

       310        320        330        340        350        360 
CHPCRNSVYQ AIRRSYSAHD MSDGYHAVIT LCILLFTHSL ALLLSSLEMV LAFVGSTGST 

       370        380        390        400        410        420 
FISFILPGSL YYFFSHKVAS PGNSSPLQLR ISRAFAAGLA IYGTVVMILC LNINIAKLSH 

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"Autophagy-deficient Schizosaccharomyces pombe mutants undergo partial sporulation during nitrogen starvation."
Mukaiyama H., Kajiwara S., Hosomi A., Giga-Hama Y., Tanaka N., Nakamura T., Takegawa K.
Microbiology 155:3816-3826(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[3]"Avt5p is required for vacuolar uptake of amino acids in the fission yeast Schizosaccharomyces pombe."
Chardwiriyapreecha S., Mukaiyama H., Sekito T., Iwaki T., Takegawa K., Kakinuma Y.
FEBS Lett. 584:2339-2345(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU329671 Genomic DNA. Translation: CAA20055.1.
PIRT39523.
RefSeqNP_595211.1. NM_001021118.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING4896.SPBC1685.07c-1.

Proteomic databases

MaxQBO74327.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC1685.07c.1; SPBC1685.07c.1:pep; SPBC1685.07c.
GeneID2540081.
KEGGspo:SPBC1685.07c.

Organism-specific databases

PomBaseSPBC1685.07c.

Phylogenomic databases

eggNOGCOG0814.
HOGENOMHOG000173527.
OMACLNINIA.
OrthoDBEOG7C8GSX.
PhylomeDBO74327.

Family and domain databases

InterProIPR013057. AA_transpt_TM.
[Graphical view]
PfamPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20801218.

Entry information

Entry nameAVT5_SCHPO
AccessionPrimary (citable) accession number: O74327
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 1, 1998
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names