O74314 (MET7_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cystathionine gamma-synthase
EC=2.5.1.48

Alternative name(s):
O-succinylhomoserine (thiol)-lyase

Gene names

ORF Names:

SPBC15D4.09c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	610 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-cystathionine from O-succinyl-L-homoserine: step 1/1.
Subcellular location	Cytoplasm. Nucleus Ref.2.
Sequence similarities	Belongs to the trans-sulfuration enzymes family. MET7 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm Nucleus
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-methionine biosynthetic process from homoserine via O-succinyl-L-homoserine and cystathionine Inferred from sequence or structural similarity. Source: GeneDB_Spombe cysteine metabolic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe nucleus Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	cystathionine gamma-synthase activity Inferred from sequence or structural similarity. Source: GeneDB_Spombe pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 610	610	Probable cystathionine gamma-synthase		PRO_0000114780
Amino acid modifications
	Modified residue	427	1	N6-(pyridoxal phosphate)lysine Potential

Sequences

	Sequence		Length	Mass (Da)
	O74314 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 129F54785ADC71EF	FASTA	610	68,968
	10 20 30 40 50 60 MSANISYLVN PPTEVGSSIP ADTEHAISVT LPTWKSNVGY EEGDPNVTTK MKSGYPRFFI 70 80 90 100 110 120 SAHVKDCISI IKKFPEIEIL KLKDYDMFLY PSLSVAKQSA AFLESKGPID SAEVIIYDAT 130 140 150 160 170 180 KGLRKHLDSI DRNRKYTEEI HIPQVYSVLF PSKYFGIAKQ FWQHTGDGIS SRRAAAFLHS 190 200 210 220 230 240 YKKIQSISEF LVAQRSISHL KSKSRSRYAS HPDLQALNTW MTNEGNQAND EMEDVSLYLE 250 260 270 280 290 300 ERYGRNLDLS LATAAKLVLR RRIAGTLKDE VDLQKALPKE GSQYLREVKG LSHDDVFLFP 310 320 330 340 350 360 TGMSAIYNTH RILRLVLDDS RKSVCFGFCY VDTLKILQKW GSGCYFYGLG NDEQLDEFEK 370 380 390 400 410 420 RLESGEKVMA LFCEFPSNPL LNSPDLVRIR KLADSYDFAV VVDETIGNFV NVEVLPLADV 430 440 450 460 470 480 VVSSLTKIFS GDSNVMGGSM VLNPSSRYYS RIKDAMKALY EDLLYDEDAL TLERNSRDFA 490 500 510 520 530 540 ERSQVINHNA ETICNLLYQN PKIKTLYYPK YNTSKEHFEA CRRENGGYGG LLSVVFHNPE 550 560 570 580 590 600 DARQFYDKIQ VAKGPSLGTN FTLASPYAIL AHYQELDWAG ENGIDRNLVR VSVGMEPSEH 610 LKNVFINALS

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

[2]

"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed: 10759889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-213, SUBCELLULAR LOCATION. Strain: ATCC 38364 / 968.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAA20484.1. AB027923 Genomic DNA. Translation: BAA87227.1.
PIR	T39485.
RefSeq	NP_596249.1. NM_001022168.1.
3D structure databases
ProteinModelPortal	O74314.
ModBase	Search...
Protein-protein interaction databases
STRING	O74314.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC15D4.09c.1; SPBC15D4.09c.1:pep; SPBC15D4.09c.
GeneID	2539747.
KEGG	spo:SPBC15D4.09c.
NMPDR	fig|4896.1.peg.2115.
Organism-specific databases
GeneDB_Spombe	SPBC15D4.09c.
Phylogenomic databases
eggNOG	fuNOG05525.
GeneTree	EFGT00050000003427.
HOGENOM	HBG634482.
OMA	DETIGNF.
OrthoDB	EOG4C8C1T.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-004275-MONOMER.
Gene expression databases
ArrayExpress	O74314.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K01739.
PANTHER	PTHR11808. Cys_Met_Meta_PP. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00868. CYS_MET_METAB_PP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MET7_SCHPO

Accession

Primary (citable) accession number: O74314
Secondary accession number(s): Q9USA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	November 1, 1998
Last modified:	December 14, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program