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Protein

Probable cystathionine gamma-synthase

Gene

SPBC15D4.09c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia (By similarity).By similarity

Catalytic activityi

O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.

Cofactori

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine.
Proteins known to be involved in this subpathway in this organism are:
  1. Probable cystathionine gamma-synthase (SPBC15D4.09c)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00051; UER00077.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cystathionine gamma-synthase (EC:2.5.1.48)
Alternative name(s):
O-succinylhomoserine (thiol)-lyase
Gene namesi
ORF Names:SPBC15D4.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC15D4.09c.
PomBaseiSPBC15D4.09c.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 610610Probable cystathionine gamma-synthasePRO_0000114780Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei427 – 4271N6-(pyridoxal phosphate)lysineSequence analysis

Proteomic databases

MaxQBiO74314.

Interactioni

Protein-protein interaction databases

MINTiMINT-4678161.

Structurei

3D structure databases

ProteinModelPortaliO74314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000193384.
InParanoidiO74314.
KOiK01739.
OMAiCEFPGNP.
OrthoDBiEOG7GFBDM.
PhylomeDBiO74314.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74314-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSANISYLVN PPTEVGSSIP ADTEHAISVT LPTWKSNVGY EEGDPNVTTK
60 70 80 90 100
MKSGYPRFFI SAHVKDCISI IKKFPEIEIL KLKDYDMFLY PSLSVAKQSA
110 120 130 140 150
AFLESKGPID SAEVIIYDAT KGLRKHLDSI DRNRKYTEEI HIPQVYSVLF
160 170 180 190 200
PSKYFGIAKQ FWQHTGDGIS SRRAAAFLHS YKKIQSISEF LVAQRSISHL
210 220 230 240 250
KSKSRSRYAS HPDLQALNTW MTNEGNQAND EMEDVSLYLE ERYGRNLDLS
260 270 280 290 300
LATAAKLVLR RRIAGTLKDE VDLQKALPKE GSQYLREVKG LSHDDVFLFP
310 320 330 340 350
TGMSAIYNTH RILRLVLDDS RKSVCFGFCY VDTLKILQKW GSGCYFYGLG
360 370 380 390 400
NDEQLDEFEK RLESGEKVMA LFCEFPSNPL LNSPDLVRIR KLADSYDFAV
410 420 430 440 450
VVDETIGNFV NVEVLPLADV VVSSLTKIFS GDSNVMGGSM VLNPSSRYYS
460 470 480 490 500
RIKDAMKALY EDLLYDEDAL TLERNSRDFA ERSQVINHNA ETICNLLYQN
510 520 530 540 550
PKIKTLYYPK YNTSKEHFEA CRRENGGYGG LLSVVFHNPE DARQFYDKIQ
560 570 580 590 600
VAKGPSLGTN FTLASPYAIL AHYQELDWAG ENGIDRNLVR VSVGMEPSEH
610
LKNVFINALS
Length:610
Mass (Da):68,968
Last modified:November 1, 1998 - v1
Checksum:i129F54785ADC71EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA20484.1.
AB027923 Genomic DNA. Translation: BAA87227.1.
PIRiT39485.
RefSeqiNP_596249.1. NM_001022168.2.

Genome annotation databases

EnsemblFungiiSPBC15D4.09c.1; SPBC15D4.09c.1:pep; SPBC15D4.09c.
GeneIDi2539747.
KEGGispo:SPBC15D4.09c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA20484.1.
AB027923 Genomic DNA. Translation: BAA87227.1.
PIRiT39485.
RefSeqiNP_596249.1. NM_001022168.2.

3D structure databases

ProteinModelPortaliO74314.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4678161.

Proteomic databases

MaxQBiO74314.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC15D4.09c.1; SPBC15D4.09c.1:pep; SPBC15D4.09c.
GeneIDi2539747.
KEGGispo:SPBC15D4.09c.

Organism-specific databases

EuPathDBiFungiDB:SPBC15D4.09c.
PomBaseiSPBC15D4.09c.

Phylogenomic databases

HOGENOMiHOG000193384.
InParanoidiO74314.
KOiK01739.
OMAiCEFPGNP.
OrthoDBiEOG7GFBDM.
PhylomeDBiO74314.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00077.

Miscellaneous databases

NextBioi20800898.
PROiO74314.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
    Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
    Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-213, SUBCELLULAR LOCATION.
    Strain: ATCC 38364 / 968.

Entry informationi

Entry nameiMET7_SCHPO
AccessioniPrimary (citable) accession number: O74314
Secondary accession number(s): Q9USA0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: November 1, 1998
Last modified: November 11, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.