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Protein

Probable cystathionine gamma-synthase

Gene

SPBC15D4.09c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia (By similarity).By similarity

Catalytic activityi

O4-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.

Cofactori

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine.
Proteins known to be involved in this subpathway in this organism are:
  1. Probable cystathionine gamma-synthase (SPBC15D4.09c)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Methionine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00051; UER00077

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cystathionine gamma-synthase (EC:2.5.1.48)
Alternative name(s):
O-succinylhomoserine (thiol)-lyase
Gene namesi
ORF Names:SPBC15D4.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC15D4.09c
PomBaseiSPBC15D4.09c

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001147801 – 610Probable cystathionine gamma-synthaseAdd BLAST610

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei427N6-(pyridoxal phosphate)lysineSequence analysis1

Proteomic databases

MaxQBiO74314
PaxDbiO74314
PRIDEiO74314

Interactioni

Protein-protein interaction databases

STRINGi4896.SPBC15D4.09c.1

Structurei

3D structure databases

ProteinModelPortaliO74314
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000193384
InParanoidiO74314
KOiK01739
OMAiYVLLAHY
OrthoDBiEOG092C18EA
PhylomeDBiO74314

Family and domain databases

Gene3Di3.40.640.101 hit
3.90.1150.101 hit
InterProiView protein in InterPro
IPR000277 Cys/Met-Metab_PyrdxlP-dep_enz
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF01053 Cys_Met_Meta_PP, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00868 CYS_MET_METAB_PP, 1 hit

Sequencei

Sequence statusi: Complete.

O74314-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSANISYLVN PPTEVGSSIP ADTEHAISVT LPTWKSNVGY EEGDPNVTTK
60 70 80 90 100
MKSGYPRFFI SAHVKDCISI IKKFPEIEIL KLKDYDMFLY PSLSVAKQSA
110 120 130 140 150
AFLESKGPID SAEVIIYDAT KGLRKHLDSI DRNRKYTEEI HIPQVYSVLF
160 170 180 190 200
PSKYFGIAKQ FWQHTGDGIS SRRAAAFLHS YKKIQSISEF LVAQRSISHL
210 220 230 240 250
KSKSRSRYAS HPDLQALNTW MTNEGNQAND EMEDVSLYLE ERYGRNLDLS
260 270 280 290 300
LATAAKLVLR RRIAGTLKDE VDLQKALPKE GSQYLREVKG LSHDDVFLFP
310 320 330 340 350
TGMSAIYNTH RILRLVLDDS RKSVCFGFCY VDTLKILQKW GSGCYFYGLG
360 370 380 390 400
NDEQLDEFEK RLESGEKVMA LFCEFPSNPL LNSPDLVRIR KLADSYDFAV
410 420 430 440 450
VVDETIGNFV NVEVLPLADV VVSSLTKIFS GDSNVMGGSM VLNPSSRYYS
460 470 480 490 500
RIKDAMKALY EDLLYDEDAL TLERNSRDFA ERSQVINHNA ETICNLLYQN
510 520 530 540 550
PKIKTLYYPK YNTSKEHFEA CRRENGGYGG LLSVVFHNPE DARQFYDKIQ
560 570 580 590 600
VAKGPSLGTN FTLASPYAIL AHYQELDWAG ENGIDRNLVR VSVGMEPSEH
610
LKNVFINALS
Length:610
Mass (Da):68,968
Last modified:November 1, 1998 - v1
Checksum:i129F54785ADC71EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAA20484.1
AB027923 Genomic DNA Translation: BAA87227.1
PIRiT39485
RefSeqiNP_596249.1, NM_001022168.2

Genome annotation databases

EnsemblFungiiSPBC15D4.09c.1; SPBC15D4.09c.1:pep; SPBC15D4.09c
GeneIDi2539747
KEGGispo:SPBC15D4.09c

Similar proteinsi

Entry informationi

Entry nameiMET7_SCHPO
AccessioniPrimary (citable) accession number: O74314
Secondary accession number(s): Q9USA0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: November 1, 1998
Last modified: February 28, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome