Reviewed, UniProtKB/Swiss-Prot O74304 (WIN1_SCHPO)

Names and origin

Protein names

Recommended name:
    MAP kinase kinase kinase win1
    EC=2.7.11.25

Gene names

Name:	win1
ORF Names:	SPAC1006.09, SPAPJ730.01, SPAC1250.06c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	1436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Activates the wis1 MAP kinase kinase by phosphorylation. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with tea4. Ref.3
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	activation of MAPKK activity Ref.1 Inferred from genetic interaction. Source: GeneDB_SPombe regulation of mitotic cell cycle Inferred from mutant phenotype. Source: GeneDB_SPombe stress-activated protein kinase signaling pathway Inferred from genetic interaction. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Ref.1 Inferred from genetic interaction. Source: GeneDB_SPombe protein binding Ref.1 Ref.3 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
tea4	O60132	1	EBI-1099995,EBI-1099982

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1436	1436	MAP kinase kinase kinase win1		PRO_0000086816
Regions
	Domain	1120 – 1406	287	Protein kinase
	Nucleotide binding	1126 – 1134	9	ATP By similarity
	Region	282 – 1123	842	Interaction with tea4
Sites
	Active site	1244	1	Proton acceptor By similarity
	Binding site	1149	1	ATP By similarity
Amino acid modifications
	Modified residue	224	1	Phosphoserine Ref.4
	Modified residue	226	1	Phosphothreonine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	O74304-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: D55736C3D99D0AAB	FASTA	1,436	163,273
	10 20 30 40 50 60 MENILDPSVV NSHILENGSR RSSINPILDS ELRDKTFEKA HRRSLTLLSS FTSSMLELPN 70 80 90 100 110 120 NGKEENHRRP SVARSSSDRS KASAKEDLFS EAFRMAEQPP AEALTISTPV DPINIDELDR 130 140 150 160 170 180 AYAVSPSDTS NLLHPPTSSS SIPIPIKNAG HSNLDHPIRP SLQSSISSNR IIKSPGIKED 190 200 210 220 230 240 DYMHRGRSIS SPMIDVEHIN STAVPSKTKN LPEKPKRSHK LRNSITFAKI EDHPERKSQL 250 260 270 280 290 300 RRLSSSLKCF DPEYDYNDPS LSIRRDSSTY YFSNVNETYD EEDSDLDSET STVNWVQSVL 310 320 330 340 350 360 NLPSLLSDDL MANPKNKERF EWQYMLTSVL TGDIVRSEKL RLRKIASSRE GRNSDYSDNL 370 380 390 400 410 420 WMEIWCWLTH RSVDSYRENL KHLRTGMVDV LLAIMNFHWD ESNELTPIVA VDNMLQKLDK 430 440 450 460 470 480 YERLYPSRRS ILQEHSLYAS ESFQHKLDVL TAYSNVTHAL EIQVNIIRSW VGNEEMDITK 490 500 510 520 530 540 NTTNSINNVS QISNGPFVER FYRETGLIRA FEQRIMTNMN SVLSKVCNTI VTYADDLKSY 550 560 570 580 590 600 GLPLIADDYM RLLSFPFRLI KEFLNLRLSC AENITSISLF TIDSLLDDLR NTMKVAVHII 610 620 630 640 650 660 QQHTVLIKPF RDDSKFVDEN QSLNNILVAS LKFYFNLLHR KVRNGCALLH FKETEILEGE 670 680 690 700 710 720 WDFLLAVCPH IEHGFQIMSK SLSSLVGEIL TNINRYLKDQ LQGPDTDDSA LITSFYIKVL 730 740 750 760 770 780 DCVRIRFRKL MSFTRILKAH LENSCEYVIK ENSLSLLIQR LEESNHVLTY TASIEHEGAY 790 800 810 820 830 840 VIVPGHLVDS PNILREVLSM TFNKGDNNFE SVPPYAVVLA PDSSICWNGH VTDLDIPEVS 850 860 870 880 890 900 ISIAPNCVRL VTLATANQLS VIEDYFISIV GDTVSLVDSA KANSSKINKQ MTKIKRNSLK 910 920 930 940 950 960 LALSLLDVIQ TIRTRYHGMN CQNLIHYSFS YAIEFAQRLM RLSILDASSI GLIRRKMIQL 970 980 990 1000 1010 1020 AISWVGFIYE DCSPTDRNTF RWTVTALEFA MIMTYGSNIL MIDKKSFEEL KEKVGKCVAL 1030 1040 1050 1060 1070 1080 LLLHFDVMGT KHAGRSMDQQ AGDIPARLVR NNSDRSRLSD NELASFVKEE VMHRIIELES 1090 1100 1110 1120 1130 1140 NRRDRLYKSQ LIGRVLDDTT KENRLLKELA SSKSNITIRW QQGGLIGSGS FGTVYRAVNL 1150 1160 1170 1180 1190 1200 DTGDLMAVKE VALHKPRISR PMIKRIKGEM LVLELFDHPN VVSYYGIEVH REKVNIFMEL 1210 1220 1230 1240 1250 1260 CQGSSLFEFL RYGRIEDELV IQVYVLQLLE GLAYIHSCGV SHQDVKPENI LFDHNGIMKF 1270 1280 1290 1300 1310 1320 TDFGSAKMSG SASTKIFEQL TQQEEEEFEK DSEFLQHLDQ NRGYSLTGTP TYMAPELILG 1330 1340 1350 1360 1370 1380 NPSERVGAMD IWSLGCVIVE MATGSPPWPR LDNHFSLMYH IAAHNPPIIP ADDQLSPLGQ 1390 1400 1410 1420 1430 NFLKRCFVSD PNQRATAAEL LMDPWVYPLR AGTEFDLMNS SVVESAPSTN GAPLEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The fission yeast mitotic regulator win1+ encodes an MAP kinase kinase kinase that phosphorylates and activates Wis1 MAP kinase kinase in response to high osmolarity." Samejima I., Mackie S., Warbrick E., Weisman R., Fantes P.A. Mol. Biol. Cell 9:2325-2335(1998) [PubMed: 9693384] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Wsh3/Tea4 is a novel cell-end factor essential for bipolar distribution of Tea1 and protects cell polarity under environmental stress in S. pombe." Tatebe H., Shimada K., Uzawa S., Morigasaki S., Shiozaki K. Curr. Biol. 15:1006-1015(2005) [PubMed: 15936270] [Abstract] Cited for: INTERACTION WITH TEA4.
[4]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-224 AND THR-226, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ223190 Genomic DNA. Translation: CAA11161.1. CU329670 Genomic DNA. Translation: CAB60239.1.
PIR	T37556. T50298. T50457.
RefSeq	NP_594856.2.
3D structure databases
HSSP	HSSP built from PDB template 1GNG based on UniProtKB P49841.
ModBase	Search...
Protein-protein interaction databases
IntAct	O74304. 1 interaction.
STRING	O74304.
Genome annotation databases
GeneID	2542988.
GenomeReviews	Gene locus win1 in contig CU329670_GR.
KEGG	spo:SPAC1006.09.
Organism-specific databases
GeneDB_Spombe	SPAC1006.09.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-003086-MON.
BRENDA	2.7.11.25. 653.
Gene expression databases
ArrayExpress	O74304.
Family and domain databases
InterPro	IPR015747. MAPKKK4. IPR017240. MAPKKK_SSK22. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
PANTHER	PTHR22986:SF60. MAPKKK4. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF037579. MAPKKK_SSK22. 1 hit.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WIN1_SCHPO

Accession

Primary (citable) accession number: O74304
Secondary accession number(s): Q9P7R1, Q9UTP1, Q9UTR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	November 1, 1998
Last modified:	September 22, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)