O74298 (LYS2_PENCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-aminoadipate-semialdehyde dehydrogenase large subunit
EC=1.2.1.31

Alternative name(s):
Alpha-aminoadipate reductase
Short name=Alpha-AR

Gene names

Name:

lys2

Organism

Penicillium chrysogenum (Penicillium notatum)

Taxonomic identifier

5076 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Penicillium › Penicillium chrysogenum complex

Protein attributes

Sequence length	1409 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH.
Catalytic activity	(S)-2-amino-6-oxohexanoate + NAD(P)+ + H2O = L-2-aminoadipate + NAD(P)H.
Cofactor	Binds 1 phosphopantetheine covalently Potential.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 1/3.
Subunit structure	Heterodimer of an alpha and a beta subunit By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family. Contains 1 acyl carrier domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Ligand	NADP Phosphopantetheine
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	lysine biosynthetic process via aminoadipic acid Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	L-aminoadipate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro phosphopantetheine binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1409	1409	L-aminoadipate-semialdehyde dehydrogenase large subunit		PRO_0000193151
Regions
	Domain	863 – 934	72	Acyl carrier
Amino acid modifications
	Modified residue	896	1	O-(pantetheine 4'-phosphoryl)serine Potential

Sequences

	Sequence		Length	Mass (Da)
	O74298 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: A85DFD397BAB29AE	FASTA	1,409	154,842
	10 20 30 40 50 60 MAVGTASLQD RLETWAQRLK NLTVSPLTRD YPDTQKTDSK RVIEAFESLQ LPKAKLTGSS 70 80 90 100 110 120 SSFIAFLTAF IILVARLTGD EDIAVGTNSN EDGRAFVIRV PIDTSESFAQ LYAKVDKAYK 130 140 150 160 170 180 EGSSQIVPLG SLRSYIQEKS KSERTPVLFR FAAYDAPASS QDYPANTFDT TDLVVNVAPG 190 200 210 220 230 240 SAEVELGAYY NQRLFSSARI AFILKQLASI ASNAAANPDE AIGRIDLMTE DQRALLPDPT 250 260 270 280 290 300 CNLNWSNFRG AIHDIFTANA ERHPEKLCVV ETQSSSSPHR EFTYRQINEA SNILGHHLVR 310 320 330 340 350 360 SGIQRGEVVM VYAYRGVDLV VAVMGILKAG ATFSVIDPAY PPERQNIYLD VARPRALVNI 370 380 390 400 410 420 AKATKDAGEL SDIVRTFIDE NLELRTEIPA LALLDDGTLA GGSINGQDVF ANDVALKSKP 430 440 450 460 470 480 TGVVVGPDSI PTLSFTSGSE GRPKGVRGRH FSLAYYFPWM SETFKLTPDE KFTMLSGIAH 490 500 510 520 530 540 DPIQRDIFTP LFLGAQLLVP AREDIQNEKL AEWIEKYGAT ITHLTPAMGQ ILVGGASAQF 550 560 570 580 590 600 PALHHAFFVG DILIKRDCRS LQGLAPNVSI VNMYGTTETQ RAVSYYEIPS YASNEGYLNN 610 620 630 640 650 660 MKDVIMAGRG MLDVQMLVVN RYDPTRLCAI GEVGEIYVRA GGLAEGYLGS PELSAKKFLN 670 680 690 700 710 720 NWFVNPEIWA EKDQAESRNE PWRQFYVGPR DRLYRSGDLG RYTPSGDVEC SGRADDQVKI 730 740 750 760 770 780 RGFRIELGEI DTHLSQHPLV RENVTLVRRD KDEEPTLVSY FVPDMNKWAS WLESKGLKDD 790 800 810 820 830 840 DSDSEGMVGL LRRFRPLRDD AREHLRTKLP TYAVPTVIIP LKRMPLNPNG KIDKPALPFP 850 860 870 880 890 900 DTAELSAAAP RRASSALQAL SETEQTLAQV WAKLIPNVTS RMIGPDDSFF DLGGHSILAQ 910 920 930 940 950 960 QMFFELRRKW RVIDISMNAI FRSPTLKGFA SEIDRLLAME SFATSDDKTL AVQAANEPDD 970 980 990 1000 1010 1020 EYSKDAVQLV NELPKTFPQR TEAMLTSEPT VFLTGATGFL GAHILRDLLT RKSPSTKVVA 1030 1040 1050 1060 1070 1080 LVRAKTEELA LERLRSTCRA YGFWDEAWTA KLQAVCGDLG KPQFGLSQSV WDDLTNRVDA 1090 1100 1110 1120 1130 1140 VIHNGALVHW VYPYATLRPA NVMGTIDALK LCASGKAKQF AFVSSTSALD KDRYVQESER 1150 1160 1170 1180 1190 1200 IIAAGGNGIS EDDDMEGSRV GLGTGYGQSK WAGEYLVKEA GRRGLRGTIV RSGYVLGDSV 1210 1220 1230 1240 1250 1260 TGTTNTDDFL IRMLKGCIQI GLRPNIFNTV NMVPVDHVAR IVIATAFHPP ATGVNVAHVT 1270 1280 1290 1300 1310 1320 GHPRLRFNQF LGALELYGYN VPQVDYVPWS TSLEQYVNDG EHNDKESQHA LMPLYHFVTS 1330 1340 1350 1360 1370 1380 DLPSNTKAPE LDDVNAATAL RADATWSGVD ASAGAGVTEE LVGLYASYLV QTGFLPAPTV 1390 1400 AGARPLPAAQ ISEEQKKTLL SVGGRGGTS

References

[1]

"Characterization of the lys2 gene of Penicillium chrysogenum encoding alpha-aminoadipic acid reductase." Casqueiro J., Gutierrez S., Banuelos O., Fierro F., Velasco J., Martin J.F. Mol. Gen. Genet. 259:549-556(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AS-P-78.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y13967 Genomic DNA. Translation: CAA74300.1.
3D structure databases
ProteinModelPortal	O74298.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00033; UER00032.
Family and domain databases
Gene3D	1.10.1200.10. 1 hit. 3.40.50.720. 1 hit.
InterPro	IPR010071. AA_adenyl_domain. IPR009081. Acyl_carrier_prot-like. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. IPR014397. L-NH2adipate-semiAld_DH_lsu. IPR013120. Male_sterile_NAD-bd. IPR016040. NAD(P)-bd_dom. IPR010080. Thioester_reductase_dom. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. PF07993. NAD_binding_4. 1 hit. PF00550. PP-binding. 1 hit. [Graphical view]
PIRSF	PIRSF001617. Alpha-AR. 1 hit.
SUPFAM	SSF47336. ACP_like. 1 hit.
TIGRFAMs	TIGR01733. AA-adenyl-dom. 1 hit. TIGR03443. alpha_am_amid. 1 hit. TIGR01746. Thioester-redct. 1 hit.
PROSITE	PS50075. ACP_DOMAIN. 1 hit. PS00455. AMP_BINDING. 1 hit. PS00012. PHOSPHOPANTETHEINE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYS2_PENCH

Accession

Primary (citable) accession number: O74298

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	November 1, 1998
Last modified:	April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program