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O74288

- ABFB_EMENI

UniProt

O74288 - ABFB_EMENI

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Protein

Alpha-L-arabinofuranosidase B

Gene
abfB, AN1571
Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Alpha-L-arabinofuranosidase involved in the degradation of arabinoxylan, a major component of plant hemicellulose. Able to hydrolyze 1,5-, 1,3- and 1,2-alpha-linkages not only in L-arabinofuranosyl oligosaccharides, but also in polysaccharides containing terminal non-reducing L-arabinofuranoses in side chains, like L-arabinan, arabinogalactan and arabinoxylan.2 Publications

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.

pH dependencei

Optimum pH is 4.8.1 Publication

Temperature dependencei

Optimum temperature is 65 degrees Celsius.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei182 – 1832Cis-peptide bond By similarity
Binding sitei225 – 2251Substrate By similarity
Active sitei227 – 2271Nucleophile By similarity
Binding sitei228 – 2281Substrate; via amide nitrogen By similarity
Binding sitei303 – 3031Substrate; via amide nitrogen By similarity
Active sitei304 – 3041Proton donor By similarity
Binding sitei427 – 4271Substrate By similarity
Binding sitei429 – 4291Substrate; via amide nitrogen By similarity
Binding sitei430 – 4301Substrate; via amide nitrogen By similarity
Binding sitei446 – 4461Substrate By similarity
Binding sitei475 – 4751Substrate By similarity
Binding sitei477 – 4771Substrate; via amide nitrogen By similarity
Binding sitei480 – 4801Substrate; via amide nitrogen By similarity
Binding sitei500 – 5001Substrate By similarity

GO - Molecular functioni

  1. alpha-L-arabinofuranosidase activity Source: UniProtKB

GO - Biological processi

  1. arabinan catabolic process Source: UniProtKB-UniPathway
  2. arabinose metabolic process Source: UniProtKB
  3. L-arabinose metabolic process Source: InterPro
  4. pectin catabolic process Source: UniProtKB
  5. xylan catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Enzyme and pathway databases

UniPathwayiUPA00667.

Protein family/group databases

CAZyiCBM42. Carbohydrate-Binding Module Family 42.
GH54. Glycoside Hydrolase Family 54.
mycoCLAPiABF54B_EMENI.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-L-arabinofuranosidase B (EC:3.2.1.55)
Short name:
ABF B
Short name:
Arabinosidase B
Gene namesi
Name:abfB
ORF Names:AN1571
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000000560: Chromosome VII

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed predictionAdd
BLAST
Chaini25 – 510486Alpha-L-arabinofuranosidase BPRO_0000394609Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi27 ↔ 37 By similarity
Disulfide bondi87 ↔ 92 By similarity
Glycosylationi89 – 891N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi182 ↔ 183 By similarity
Disulfide bondi412 ↔ 450 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Inductioni

Expressed in presence of L-arabinol and repressed in presence of glucose and glycerol. Expression is also pH regulated probably through the action of the pacC transcription factor and is higher at acidic pHs.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi162425.CADANIAP00008202.

Structurei

3D structure databases

ProteinModelPortaliO74288.
SMRiO74288. Positions 25-510.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 342318Catalytic By similarityAdd
BLAST
Regioni343 – 510168ABD By similarityAdd
BLAST

Domaini

Organized into two domains: an N-terminal catalytic domain and a C-terminal arabinose-binding domain (ABD) By similarity.

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG83819.
HOGENOMiHOG000187007.
OMAiNIVAAKY.
OrthoDBiEOG7DFXNQ.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR015289. A-L-arabinofuranosidase_B_cat.
IPR007934. AbfB.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
[Graphical view]
PfamiPF05270. AbfB. 1 hit.
PF09206. ArabFuran-catal. 1 hit.
[Graphical view]
SUPFAMiSSF110221. SSF110221. 1 hit.
SSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O74288-1 [UniParc]FASTAAdd to Basket

« Hide

MTMSRSSRSS VLALALATGS LVAAGPCDIY SSGGTPCIAA HSTTRALYSS    50
YNGPLYQVQR ASDGTTTTIT PLSAGGVADA SAQDAFCENT TCLITIIYDQ 100
SGNGNDLTQA PPGGFNGPDV GGYDNLAGAI GAPVTLNGKK AYGVFVSPGT 150
GYRNNEAIGT ATGDEPEGMY AVLDGTHYND GCCFDYGNAE TSSLDTGNGH 200
MEAIYYGTNT AWGYGAGNGP WIMADLENGL FSGQSSDYNA GDPSISYRFV 250
TAILKGGPNL WALRGGNAAS GSLSTYYNGI RPTDASGYNP MSKEGAIILG 300
IGGDNSVSAQ GTFYEGAMTD GYPDDATENS VQADIVAAKY ATTSLISGPA 350
LTVGDTVSLK VTTSGYDTRY IAHTGSTINT QVVSSSSSST LKQQASWTVR 400
TGLASTAAAN GCVSFESVDT PGSYIRHSNF ALLLNANDGT KLFSEDATFC 450
PQDSFNDDGT NSIRSWNYPT RYWRHYENVL YVASNGGVNT FDAATAFTDD 500
VSWVVADGFA 510
Length:510
Mass (Da):52,942
Last modified:November 1, 1998 - v1
Checksum:i54011DA6AD3BFC22
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y13759 Genomic DNA. Translation: CAA74084.1.
AACD01000025 Genomic DNA. Translation: EAA64278.1.
BN001307 Genomic DNA. Translation: CBF85134.1.
RefSeqiXP_659175.1. XM_654083.1.

Genome annotation databases

EnsemblFungiiCADANIAT00008202; CADANIAP00008202; CADANIAG00008202.
GeneIDi2875522.
KEGGiani:AN1571.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y13759 Genomic DNA. Translation: CAA74084.1 .
AACD01000025 Genomic DNA. Translation: EAA64278.1 .
BN001307 Genomic DNA. Translation: CBF85134.1 .
RefSeqi XP_659175.1. XM_654083.1.

3D structure databases

ProteinModelPortali O74288.
SMRi O74288. Positions 25-510.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 162425.CADANIAP00008202.

Protein family/group databases

CAZyi CBM42. Carbohydrate-Binding Module Family 42.
GH54. Glycoside Hydrolase Family 54.
mycoCLAPi ABF54B_EMENI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADANIAT00008202 ; CADANIAP00008202 ; CADANIAG00008202 .
GeneIDi 2875522.
KEGGi ani:AN1571.2.

Phylogenomic databases

eggNOGi NOG83819.
HOGENOMi HOG000187007.
OMAi NIVAAKY.
OrthoDBi EOG7DFXNQ.

Enzyme and pathway databases

UniPathwayi UPA00667 .

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR015289. A-L-arabinofuranosidase_B_cat.
IPR007934. AbfB.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
[Graphical view ]
Pfami PF05270. AbfB. 1 hit.
PF09206. ArabFuran-catal. 1 hit.
[Graphical view ]
SUPFAMi SSF110221. SSF110221. 1 hit.
SSF49899. SSF49899. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The abfB gene encoding the major alpha-L-arabinofuranosidase of Aspergillus nidulans: nucleotide sequence, regulation and construction of a disrupted strain."
    Gielkens M., Gonzalez-Candelas L., Sanchez-Torres P., van de Vondervoort P., de Graaff L., Visser J., Ramon D.
    Microbiology 145:735-741(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, SUBCELLULAR LOCATION, FUNCTION.
    Strain: ArgB2, biA1 and MetG1.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  3. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
    Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
    , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
    Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  4. "Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls."
    Bauer S., Vasu P., Persson S., Mort A.J., Somerville C.R.
    Proc. Natl. Acad. Sci. U.S.A. 103:11417-11422(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiABFB_EMENI
AccessioniPrimary (citable) accession number: O74288
Secondary accession number(s): C8VN12, Q5BD09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: November 1, 1998
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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