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Protein

Enolase

Gene
N/A
Organism
Cunninghamella elegans
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei159SubstrateBy similarity1
Binding sitei168SubstrateBy similarity1
Active sitei211Proton donorBy similarity1
Metal bindingi246MagnesiumBy similarity1
Metal bindingi295MagnesiumBy similarity1
Binding sitei295SubstrateBy similarity1
Metal bindingi320MagnesiumBy similarity1
Binding sitei320SubstrateBy similarity1
Active sitei345Proton acceptorBy similarity1
Binding sitei396SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
OrganismiCunninghamella elegans
Taxonomic identifieri4853 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisMucoromycotaMucoromycotinaMucoralesCunninghamellaceaeCunninghamella

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000134048‹1 – 436EnolaseAdd BLAST›436

Proteomic databases

PRIDEiO74286

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO74286
SMRiO74286
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni372 – 375Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Fragment.

O74286-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
STITKVHARQ IFDSRGNPTV EVEVTTDKGV FRAGVPSGAS TGVHEALELR
60 70 80 90 100
DGVKSDYLGK GVLKAVGNVN TIINEELVKA NLSVVDQKAV DDFLIQLDGT
110 120 130 140 150
ENKEKLGANA ILGVSLAVAK AGAAEKGVPF YVHIADLAGS KKPFVLPVPA
160 170 180 190 200
FNVINGGSHA GNKLAMQEFM IMPTGAKSFS EAMKLGSEVY HTLKKVINEK
210 220 230 240 250
YGQDATNVGD EGGFAPNIQD NQEGLELLVT AIEKAGYTGK IKVAMDCAAS
260 270 280 290 300
DFYKDGKYDL DFKNPNSDPS TYLTGQDLTD LYNSYAGKYP IVSIEDAFDQ
310 320 330 340 350
DDWENWAHMN ASADYQLVGD DLTVTNPKRI ATAVEKKACN ALLLKVNQIG
360 370 380 390 400
TLTESIQAAL DSQKAGWGVM VSHRSGETED TSIASIVVGL RTGQIKTGAP
410 420 430
CRSERLAKYN ELLRIEEELG DAAIYAGEHF RKAHDL
Length:436
Mass (Da):46,878
Last modified:November 1, 1998 - v1
Checksum:i32BA0B8E5C801738
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17298 mRNA Translation: CAA76735.1

Similar proteinsi

Entry informationi

Entry nameiENO_CUNEL
AccessioniPrimary (citable) accession number: O74286
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

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