O74267 (GLY1_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Low-specificity L-threonine aldolase
EC=4.1.2.48

Gene names

Name:	GLY1
Ordered Locus Names:	AFR366W

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-threonine = glycine + acetaldehyde. L-allo-threonine = glycine + acetaldehyde.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid degradation; L-threonine degradation via aldolase pathway; acetaldehyde and glycine from L-threonine: step 1/1.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the threonine aldolase family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro threonine aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Low-specificity L-threonine aldolase		PRO_0000121569
Amino acid modifications
	Modified residue	214	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	14	1	V → A in CAA06545. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O74267 [UniParc]. Last modified July 19, 2004. Version 2. Checksum: 5053E8DA8A071EA8	FASTA	382	42,628
	10 20 30 40 50 60 MNQDMELPEA YTSVSNDFRS DTFTTPTREM IEAALTATIG DAVYQEDIDT LKLEQHVAKL 70 80 90 100 110 120 AGMEAGMFCV SGTLSNQIAL RTHLTQPPYS ILCDYRAHVY THEAAGLAIL SQAMVTPVIP 130 140 150 160 170 180 SNGNYLTLED IKKHYIPDDG DIHGAPTKVI SLENTLHGII HPLEELVRIK AWCMENDLRL 190 200 210 220 230 240 HCDGARIWNA SAESGVPLKQ YGELFDSISI CLSKSMGAPM GSILVGSHKF IKKANHFRKQ 250 260 270 280 290 300 QGGGVRQSGM MCKMAMVAIQ GDWKGKMRRS HRMAHELARF CAEHGIPLES PADTNFVFLD 310 320 330 340 350 360 LQKSKMNPDV LVKKSLKYGC KLMGGRVSFH YQISEESLEK IKQAILEAFE YSKKNPYDEN 370 380 GPTKIYRSES ADAVGEIKTY KY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Threonine aldolase overexpression plus threonine supplementation enhanced riboflavin production in Ashbya gossypii." Monschau N., Sahm H., Stahmann K.-P. Appl. Environ. Microbiol. 64:4283-4290(1998) [PubMed: 9797278] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
[2]	"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ005442 Genomic DNA. Translation: CAA06545.1. AE016819 Genomic DNA. Translation: AAS53737.1.
RefSeq	NP_985913.2. NM_211268.2.
3D structure databases
ProteinModelPortal	O74267.
ModBase	Search...
Protein-protein interaction databases
STRING	O74267.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4622183.
GenomeReviews	Gene locus AFR366W in contig AE016819_GR.
KEGG	ago:AGOS_AFR366W.
NMPDR	fig|33169.1.peg.3558.
Organism-specific databases
AGD	AFR366W.
Phylogenomic databases
eggNOG	fuNOG04456.
HOGENOM	HBG293912.
OMA	APMGSIL.
OrthoDB	EOG4J6W0B.
PhylomeDB	O74267.
Enzyme and pathway databases
BioCyc	AGOS-XXX-01:AGOS-XXX-01-003558-MONOMER.
Family and domain databases
InterPro	IPR001597. ArAA_b-elim_lyase/Thr_aldolase. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. IPR023603. Threonine_aldolase. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K01620.
Pfam	PF01212. Beta_elim_lyase. 1 hit. [Graphical view]
PIRSF	PIRSF017617. Thr_aldolase. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLY1_ASHGO

Accession

Primary (citable) accession number: O74267
Secondary accession number(s): Q753E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program