SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O74254

- AMYG_CANAL

UniProt

O74254 - AMYG_CANAL

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glucoamylase 1

Gene
GAM1, GCA1, CaO19.12365, CaO19.4899
Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal 1,4-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.
Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei462 – 4621 By similarity
Active sitei465 – 4651 By similarity
Active sitei628 – 6281Proton donor By similarity

GO - Molecular functioni

  1. alpha-glucosidase activity Source: CGD
  2. amylase activity Source: CGD
  3. carbohydrate binding Source: InterPro
  4. glucan 1,4-alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate catabolic process Source: CGD
  2. filamentous growth Source: CGD
  3. filamentous growth of a population of unicellular organisms Source: CGD
  4. fungal-type cell wall polysaccharide biosynthetic process Source: CGD
  5. induction by symbiont of host immune response Source: CGD
  6. polysaccharide catabolic process Source: UniProtKB-KW
  7. single-species biofilm formation on inanimate substrate Source: CGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase 1 (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:GAM1
Synonyms:GCA1
ORF Names:CaO19.12365, CaO19.4899
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
ProteomesiUP000000559: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0066397. GCA1.

Subcellular locationi

GO - Cellular componenti

  1. cell wall Source: UniProtKB-SubCell
  2. endoplasmic reticulum lumen Source: CGD
  3. extracellular region Source: CGD
  4. plasma membrane Source: CGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed predictionAdd
BLAST
Chaini21 – 946926Glucoamylase 1PRO_0000018585Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi51 – 511N-linked (GlcNAc...) Reviewed prediction
Glycosylationi68 – 681N-linked (GlcNAc...) Reviewed prediction
Glycosylationi97 – 971N-linked (GlcNAc...) Reviewed prediction
Glycosylationi187 – 1871N-linked (GlcNAc...) Reviewed prediction
Glycosylationi244 – 2441N-linked (GlcNAc...) Reviewed prediction
Glycosylationi373 – 3731N-linked (GlcNAc...) Reviewed prediction
Glycosylationi393 – 3931N-linked (GlcNAc...) Reviewed prediction
Glycosylationi406 – 4061N-linked (GlcNAc...) Reviewed prediction
Glycosylationi437 – 4371N-linked (GlcNAc...) Reviewed prediction
Glycosylationi505 – 5051N-linked (GlcNAc...) Reviewed prediction
Glycosylationi570 – 5701N-linked (GlcNAc...) Reviewed prediction
Glycosylationi772 – 7721N-linked (GlcNAc...) Reviewed prediction
Glycosylationi801 – 8011N-linked (GlcNAc...) Reviewed prediction
Glycosylationi895 – 8951N-linked (GlcNAc...) Reviewed prediction
Glycosylationi912 – 9121N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi5476.CAL0005531.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi519 – 53214Ser/Thr-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1501.
KOiK01187.
OrthoDBiEOG77T1CZ.

Family and domain databases

InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O74254-1 [UniParc]FASTAAdd to Basket

« Hide

MKLLSKVFVT ALGLTSIVNA APTSSSSAEE AQKTVPVELS IGVKQLPNIH    50
NDSAVDANAV AKGYSLVNVS LTARGLTGIL KLKEATNIYG YDFEYLNLSV 100
EYQSDTRLNV HIEPTDLTDV FVLPEELVVK PKLEGDAKTF NFENSDLVFE 150
YDEEDFGFEV LRSSTREVLF STKGNPLVFS NQFIQFNTTL PKGHSITGLG 200
ESIHGSLNEP GVVKTLYAND IADPIDGNIY GVHPVYYDQR YDTNTTHGVY 250
WRTSAIQEVV VGETSLTWRA LSGVIDLYFF SGPDPKDVIQ QYVSEIGLPA 300
MQPYWALGYH QCRWGYDTVE SLETVVENFK KFDIPLETIW SDIDYMDGYK 350
DFTNDPYRFP TDKFRKFLDD LHNNSQHYVP IFDAAIYVPN PNNATDDDYE 400
PFHLGNESDV FLKNPDGSLY IGAVWPGYTV FPDFLANNTQ EYWNKMFKDW 450
YERIPFDGIW TDMNEVSSFC VGSCGTGRYF DNPVHPPFEV GYSGSDYPLG 500
FDKSNASEWK SISEAAAATK TTTTTSSSTS TSIDGKNTLA PGKANINYPP 550
YAINNNQGDH GLATHAISPN ATHADGTVEY DIHNIYGLIQ ERAIYEALLE 600
IHPNKRPFII GRSSFAGSGK YMGHWGGDNY ADYYMMYFSI PQALSMGLSG 650
IPFFGVDACG FNGNTDMELC SRWMQLASFF PFYRNHNVLG AIPQEPYVWE 700
GVMNATKTSI NVRYSLLPYY YTLLHESHVT GIPIMRAFNW QFPYSKELAG 750
VDTQFFVGDA LLVTPVLEPG VNHTKGIFPG ENAVYYDFYT HKKQKFTAGK 800
NETLAAPLGH IPLHIKGGNI IPTQEPGYTT TESRKNPFGL LVALDAEGTA 850
SGKLYLDDGE SVDVEEALYV DFVASKNKLV ASVFGEYEVR QPLANVTILG 900
VDSEPKKVLF NNETVSHNYE NGAVYLTDLE KFTKEGAFAE EFSIQW 946
Length:946
Mass (Da):105,675
Last modified:October 19, 2011 - v2
Checksum:iA347712E0F974452
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti7 – 82VF → FI in allele CaO19.12365.
Natural varianti102 – 1021Y → S in allele CaO19.12365.
Natural varianti113 – 1131E → D in allele CaO19.12365.
Natural varianti144 – 1441N → T in allele CaO19.12365.
Natural varianti242 – 2421D → N in allele CaO19.12365.
Natural varianti248 – 2481G → A in allele CaO19.12365.
Natural varianti397 – 3971D → N in allele CaO19.12365.
Natural varianti477 – 4771G → D in allele CaO19.12365.
Natural varianti544 – 5441A → G in allele CaO19.12365.
Natural varianti561 – 5611G → D in allele CaO19.12365.
Natural varianti745 – 7451S → N in allele CaO19.12365.
Natural varianti752 – 7521D → E in allele CaO19.12365.
Natural varianti777 – 7771I → V in allele CaO19.12365.
Natural varianti783 – 7831A → V in allele CaO19.12365.
Natural varianti826 – 8261P → A in allele CaO19.12365.
Natural varianti832 – 8321E → G in allele CaO19.12365.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti529 – 5291T → A in AAC31968. 1 Publication
Sequence conflicti556 – 5561N → D in AAC31968. 1 Publication
Sequence conflicti701 – 7011G → A in AAC31968. 1 Publication
Sequence conflicti704 – 7041N → K in AAC31968. 1 Publication
Sequence conflicti918 – 9181N → K in AAC31968. 1 Publication
Sequence conflicti943 – 9442SI → TL in AAC31968. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF082188 Genomic DNA. Translation: AAC31968.1.
AACQ01000001 Genomic DNA. Translation: EAL04887.1.
AACQ01000002 Genomic DNA. Translation: EAL04694.1.
RefSeqiXP_723393.1. XM_718300.1.
XP_723581.1. XM_718488.1.

Genome annotation databases

GeneIDi3634903.
3635124.
KEGGical:CaO19.12365.
cal:CaO19.4899.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF082188 Genomic DNA. Translation: AAC31968.1 .
AACQ01000001 Genomic DNA. Translation: EAL04887.1 .
AACQ01000002 Genomic DNA. Translation: EAL04694.1 .
RefSeqi XP_723393.1. XM_718300.1.
XP_723581.1. XM_718488.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5476.CAL0005531.

Protein family/group databases

CAZyi GH31. Glycoside Hydrolase Family 31.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3634903.
3635124.
KEGGi cal:CaO19.12365.
cal:CaO19.4899.

Organism-specific databases

CGDi CAL0066397. GCA1.

Phylogenomic databases

eggNOGi COG1501.
KOi K01187.
OrthoDBi EOG77T1CZ.

Family and domain databases

InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF01055. Glyco_hydro_31. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and cloning of GCA1, a gene that encodes a cell surface glucoamylase from Candida albicans."
    Sturtevant J., Dixon F., Wadsworth E., Latge J.-P., Zhao X.-J., Calderone R.
    Med. Mycol. 37:357-366(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 193-213; 776-792 AND 817-835.
    Strain: SC5314 / ATCC MYA-2876.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.

Entry informationi

Entry nameiAMYG_CANAL
AccessioniPrimary (citable) accession number: O74254
Secondary accession number(s): Q5AP64, Q5APQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: October 19, 2011
Last modified: April 16, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi