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O74254 (AMYG_CANAL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucoamylase 1

EC=3.2.1.3
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene names
Name:GAM1
Synonyms:GCA1
ORF Names:CaO19.12365, CaO19.4899
OrganismCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]
Taxonomic identifier237561 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

Protein attributes

Sequence length946 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal 1,4-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Subcellular location

Secretedcell wall. Membrane; Peripheral membrane protein.

Post-translational modification

The N-terminus is blocked.

Sequence similarities

Belongs to the glycosyl hydrolase 31 family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cell wall biogenesis/degradation
Polysaccharide degradation
   Cellular componentMembrane
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate catabolic process

Inferred from sequence or structural similarity Ref.1. Source: CGD

filamentous growth

Inferred from mutant phenotype PubMed 17933909. Source: CGD

filamentous growth of a population of unicellular organisms

Inferred from mutant phenotype PubMed 17933909. Source: CGD

fungal-type cell wall polysaccharide biosynthetic process

Inferred from mutant phenotype PubMed 17933909. Source: CGD

induction by symbiont of host immune response

Inferred from mutant phenotype PubMed 17933909. Source: CGD

polysaccharide catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

single-species biofilm formation on inanimate substrate

Inferred from mutant phenotype PubMed 19529758. Source: CGD

   Cellular_componentcell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

endoplasmic reticulum lumen

Inferred from sequence alignment PubMed 17933909. Source: CGD

extracellular region

Inferred from direct assay PubMed 20641015. Source: CGD

plasma membrane

Inferred from sequence or structural similarity Ref.1. Source: CGD

   Molecular_functionalpha-glucosidase activity

Inferred from sequence alignment PubMed 17933909. Source: CGD

amylase activity

Inferred from sequence or structural similarity Ref.1. Source: CGD

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

glucan 1,4-alpha-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 946926Glucoamylase 1
PRO_0000018585

Regions

Compositional bias519 – 53214Ser/Thr-rich

Sites

Active site4621 By similarity
Active site4651 By similarity
Active site6281Proton donor By similarity

Amino acid modifications

Glycosylation511N-linked (GlcNAc...) Potential
Glycosylation681N-linked (GlcNAc...) Potential
Glycosylation971N-linked (GlcNAc...) Potential
Glycosylation1871N-linked (GlcNAc...) Potential
Glycosylation2441N-linked (GlcNAc...) Potential
Glycosylation3731N-linked (GlcNAc...) Potential
Glycosylation3931N-linked (GlcNAc...) Potential
Glycosylation4061N-linked (GlcNAc...) Potential
Glycosylation4371N-linked (GlcNAc...) Potential
Glycosylation5051N-linked (GlcNAc...) Potential
Glycosylation5701N-linked (GlcNAc...) Potential
Glycosylation7721N-linked (GlcNAc...) Potential
Glycosylation8011N-linked (GlcNAc...) Potential
Glycosylation8951N-linked (GlcNAc...) Potential
Glycosylation9121N-linked (GlcNAc...) Potential

Natural variations

Natural variant7 – 82VF → FI in allele CaO19.12365.
Natural variant1021Y → S in allele CaO19.12365.
Natural variant1131E → D in allele CaO19.12365.
Natural variant1441N → T in allele CaO19.12365.
Natural variant2421D → N in allele CaO19.12365.
Natural variant2481G → A in allele CaO19.12365.
Natural variant3971D → N in allele CaO19.12365.
Natural variant4771G → D in allele CaO19.12365.
Natural variant5441A → G in allele CaO19.12365.
Natural variant5611G → D in allele CaO19.12365.
Natural variant7451S → N in allele CaO19.12365.
Natural variant7521D → E in allele CaO19.12365.
Natural variant7771I → V in allele CaO19.12365.
Natural variant7831A → V in allele CaO19.12365.
Natural variant8261P → A in allele CaO19.12365.
Natural variant8321E → G in allele CaO19.12365.

Experimental info

Sequence conflict5291T → A in AAC31968. Ref.1
Sequence conflict5561N → D in AAC31968. Ref.1
Sequence conflict7011G → A in AAC31968. Ref.1
Sequence conflict7041N → K in AAC31968. Ref.1
Sequence conflict9181N → K in AAC31968. Ref.1
Sequence conflict943 – 9442SI → TL in AAC31968. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O74254 [UniParc].

Last modified October 19, 2011. Version 2.
Checksum: A347712E0F974452

FASTA946105,675
        10         20         30         40         50         60 
MKLLSKVFVT ALGLTSIVNA APTSSSSAEE AQKTVPVELS IGVKQLPNIH NDSAVDANAV 

        70         80         90        100        110        120 
AKGYSLVNVS LTARGLTGIL KLKEATNIYG YDFEYLNLSV EYQSDTRLNV HIEPTDLTDV 

       130        140        150        160        170        180 
FVLPEELVVK PKLEGDAKTF NFENSDLVFE YDEEDFGFEV LRSSTREVLF STKGNPLVFS 

       190        200        210        220        230        240 
NQFIQFNTTL PKGHSITGLG ESIHGSLNEP GVVKTLYAND IADPIDGNIY GVHPVYYDQR 

       250        260        270        280        290        300 
YDTNTTHGVY WRTSAIQEVV VGETSLTWRA LSGVIDLYFF SGPDPKDVIQ QYVSEIGLPA 

       310        320        330        340        350        360 
MQPYWALGYH QCRWGYDTVE SLETVVENFK KFDIPLETIW SDIDYMDGYK DFTNDPYRFP 

       370        380        390        400        410        420 
TDKFRKFLDD LHNNSQHYVP IFDAAIYVPN PNNATDDDYE PFHLGNESDV FLKNPDGSLY 

       430        440        450        460        470        480 
IGAVWPGYTV FPDFLANNTQ EYWNKMFKDW YERIPFDGIW TDMNEVSSFC VGSCGTGRYF 

       490        500        510        520        530        540 
DNPVHPPFEV GYSGSDYPLG FDKSNASEWK SISEAAAATK TTTTTSSSTS TSIDGKNTLA 

       550        560        570        580        590        600 
PGKANINYPP YAINNNQGDH GLATHAISPN ATHADGTVEY DIHNIYGLIQ ERAIYEALLE 

       610        620        630        640        650        660 
IHPNKRPFII GRSSFAGSGK YMGHWGGDNY ADYYMMYFSI PQALSMGLSG IPFFGVDACG 

       670        680        690        700        710        720 
FNGNTDMELC SRWMQLASFF PFYRNHNVLG AIPQEPYVWE GVMNATKTSI NVRYSLLPYY 

       730        740        750        760        770        780 
YTLLHESHVT GIPIMRAFNW QFPYSKELAG VDTQFFVGDA LLVTPVLEPG VNHTKGIFPG 

       790        800        810        820        830        840 
ENAVYYDFYT HKKQKFTAGK NETLAAPLGH IPLHIKGGNI IPTQEPGYTT TESRKNPFGL 

       850        860        870        880        890        900 
LVALDAEGTA SGKLYLDDGE SVDVEEALYV DFVASKNKLV ASVFGEYEVR QPLANVTILG 

       910        920        930        940 
VDSEPKKVLF NNETVSHNYE NGAVYLTDLE KFTKEGAFAE EFSIQW 

« Hide

References

« Hide 'large scale' references
[1]"Identification and cloning of GCA1, a gene that encodes a cell surface glucoamylase from Candida albicans."
Sturtevant J., Dixon F., Wadsworth E., Latge J.-P., Zhao X.-J., Calderone R.
Med. Mycol. 37:357-366(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 193-213; 776-792 AND 817-835.
Strain: SC5314 / ATCC MYA-2876.
[2]"The diploid genome sequence of Candida albicans."
Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S.
Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF082188 Genomic DNA. Translation: AAC31968.1.
AACQ01000001 Genomic DNA. Translation: EAL04887.1.
AACQ01000002 Genomic DNA. Translation: EAL04694.1.
RefSeqXP_723393.1. XM_718300.1.
XP_723581.1. XM_718488.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5476.CAL0005531.

Protein family/group databases

CAZyGH31. Glycoside Hydrolase Family 31.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3634903.
3635124.
KEGGcal:CaO19.12365.
cal:CaO19.4899.

Organism-specific databases

CGDCAL0066397. GCA1.

Phylogenomic databases

eggNOGCOG1501.
KOK01187.
OrthoDBEOG77T1CZ.

Family and domain databases

InterProIPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMYG_CANAL
AccessionPrimary (citable) accession number: O74254
Secondary accession number(s): Q5AP64, Q5APQ9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: October 19, 2011
Last modified: April 16, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Candida albicans

Candida albicans: entries and gene names