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O74254

- AMYG_CANAL

UniProt

O74254 - AMYG_CANAL

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Protein

Glucoamylase 1

Gene

GAM1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal 1,4-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.PROSITE-ProRule annotation
Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei462 – 4621By similarity
Active sitei465 – 4651By similarity
Active sitei628 – 6281Proton donorBy similarity

GO - Molecular functioni

  1. alpha-1,4-glucosidase activity Source: CGD
  2. carbohydrate binding Source: InterPro
  3. glucan 1,4-alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. filamentous growth Source: CGD
  3. filamentous growth of a population of unicellular organisms Source: CGD
  4. fungal-type cell wall polysaccharide biosynthetic process Source: CGD
  5. induction by symbiont of host immune response Source: CGD
  6. polysaccharide catabolic process Source: UniProtKB-KW
  7. single-species biofilm formation on inanimate substrate Source: CGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase 1 (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:GAM1
Synonyms:GCA1
ORF Names:CaO19.12365, CaO19.4899
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
ProteomesiUP000000559: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0066397. GCA1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: CGD
  2. extracellular region Source: CGD
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 946926Glucoamylase 1PRO_0000018585Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi51 – 511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi68 – 681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi97 – 971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi244 – 2441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi373 – 3731N-linked (GlcNAc...)Sequence Analysis
Glycosylationi393 – 3931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi406 – 4061N-linked (GlcNAc...)Sequence Analysis
Glycosylationi437 – 4371N-linked (GlcNAc...)Sequence Analysis
Glycosylationi505 – 5051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi570 – 5701N-linked (GlcNAc...)Sequence Analysis
Glycosylationi772 – 7721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi801 – 8011N-linked (GlcNAc...)Sequence Analysis
Glycosylationi895 – 8951N-linked (GlcNAc...)Sequence Analysis
Glycosylationi912 – 9121N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi5476.CAL0005531.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi519 – 53214Ser/Thr-richAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 31 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1501.
InParanoidiO74254.
KOiK01187.
OrthoDBiEOG77T1CZ.

Family and domain databases

InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O74254-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLLSKVFVT ALGLTSIVNA APTSSSSAEE AQKTVPVELS IGVKQLPNIH
60 70 80 90 100
NDSAVDANAV AKGYSLVNVS LTARGLTGIL KLKEATNIYG YDFEYLNLSV
110 120 130 140 150
EYQSDTRLNV HIEPTDLTDV FVLPEELVVK PKLEGDAKTF NFENSDLVFE
160 170 180 190 200
YDEEDFGFEV LRSSTREVLF STKGNPLVFS NQFIQFNTTL PKGHSITGLG
210 220 230 240 250
ESIHGSLNEP GVVKTLYAND IADPIDGNIY GVHPVYYDQR YDTNTTHGVY
260 270 280 290 300
WRTSAIQEVV VGETSLTWRA LSGVIDLYFF SGPDPKDVIQ QYVSEIGLPA
310 320 330 340 350
MQPYWALGYH QCRWGYDTVE SLETVVENFK KFDIPLETIW SDIDYMDGYK
360 370 380 390 400
DFTNDPYRFP TDKFRKFLDD LHNNSQHYVP IFDAAIYVPN PNNATDDDYE
410 420 430 440 450
PFHLGNESDV FLKNPDGSLY IGAVWPGYTV FPDFLANNTQ EYWNKMFKDW
460 470 480 490 500
YERIPFDGIW TDMNEVSSFC VGSCGTGRYF DNPVHPPFEV GYSGSDYPLG
510 520 530 540 550
FDKSNASEWK SISEAAAATK TTTTTSSSTS TSIDGKNTLA PGKANINYPP
560 570 580 590 600
YAINNNQGDH GLATHAISPN ATHADGTVEY DIHNIYGLIQ ERAIYEALLE
610 620 630 640 650
IHPNKRPFII GRSSFAGSGK YMGHWGGDNY ADYYMMYFSI PQALSMGLSG
660 670 680 690 700
IPFFGVDACG FNGNTDMELC SRWMQLASFF PFYRNHNVLG AIPQEPYVWE
710 720 730 740 750
GVMNATKTSI NVRYSLLPYY YTLLHESHVT GIPIMRAFNW QFPYSKELAG
760 770 780 790 800
VDTQFFVGDA LLVTPVLEPG VNHTKGIFPG ENAVYYDFYT HKKQKFTAGK
810 820 830 840 850
NETLAAPLGH IPLHIKGGNI IPTQEPGYTT TESRKNPFGL LVALDAEGTA
860 870 880 890 900
SGKLYLDDGE SVDVEEALYV DFVASKNKLV ASVFGEYEVR QPLANVTILG
910 920 930 940
VDSEPKKVLF NNETVSHNYE NGAVYLTDLE KFTKEGAFAE EFSIQW
Length:946
Mass (Da):105,675
Last modified:October 19, 2011 - v2
Checksum:iA347712E0F974452
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti529 – 5291T → A in AAC31968. (PubMed:10520161)Curated
Sequence conflicti556 – 5561N → D in AAC31968. (PubMed:10520161)Curated
Sequence conflicti701 – 7011G → A in AAC31968. (PubMed:10520161)Curated
Sequence conflicti704 – 7041N → K in AAC31968. (PubMed:10520161)Curated
Sequence conflicti918 – 9181N → K in AAC31968. (PubMed:10520161)Curated
Sequence conflicti943 – 9442SI → TL in AAC31968. (PubMed:10520161)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti7 – 82VF → FI in allele CaO19.12365.
Natural varianti102 – 1021Y → S in allele CaO19.12365.
Natural varianti113 – 1131E → D in allele CaO19.12365.
Natural varianti144 – 1441N → T in allele CaO19.12365.
Natural varianti242 – 2421D → N in allele CaO19.12365.
Natural varianti248 – 2481G → A in allele CaO19.12365.
Natural varianti397 – 3971D → N in allele CaO19.12365.
Natural varianti477 – 4771G → D in allele CaO19.12365.
Natural varianti544 – 5441A → G in allele CaO19.12365.
Natural varianti561 – 5611G → D in allele CaO19.12365.
Natural varianti745 – 7451S → N in allele CaO19.12365.
Natural varianti752 – 7521D → E in allele CaO19.12365.
Natural varianti777 – 7771I → V in allele CaO19.12365.
Natural varianti783 – 7831A → V in allele CaO19.12365.
Natural varianti826 – 8261P → A in allele CaO19.12365.
Natural varianti832 – 8321E → G in allele CaO19.12365.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF082188 Genomic DNA. Translation: AAC31968.1.
AACQ01000001 Genomic DNA. Translation: EAL04887.1.
AACQ01000002 Genomic DNA. Translation: EAL04694.1.
RefSeqiXP_723393.1. XM_718300.1.
XP_723581.1. XM_718488.1.

Genome annotation databases

GeneIDi3634903.
3635124.
KEGGical:CaO19.12365.
cal:CaO19.4899.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF082188 Genomic DNA. Translation: AAC31968.1 .
AACQ01000001 Genomic DNA. Translation: EAL04887.1 .
AACQ01000002 Genomic DNA. Translation: EAL04694.1 .
RefSeqi XP_723393.1. XM_718300.1.
XP_723581.1. XM_718488.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5476.CAL0005531.

Protein family/group databases

CAZyi GH31. Glycoside Hydrolase Family 31.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3634903.
3635124.
KEGGi cal:CaO19.12365.
cal:CaO19.4899.

Organism-specific databases

CGDi CAL0066397. GCA1.

Phylogenomic databases

eggNOGi COG1501.
InParanoidi O74254.
KOi K01187.
OrthoDBi EOG77T1CZ.

Family and domain databases

InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF01055. Glyco_hydro_31. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and cloning of GCA1, a gene that encodes a cell surface glucoamylase from Candida albicans."
    Sturtevant J., Dixon F., Wadsworth E., Latge J.-P., Zhao X.-J., Calderone R.
    Med. Mycol. 37:357-366(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 193-213; 776-792 AND 817-835.
    Strain: SC5314 / ATCC MYA-2876.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.

Entry informationi

Entry nameiAMYG_CANAL
AccessioniPrimary (citable) accession number: O74254
Secondary accession number(s): Q5AP64, Q5APQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: October 19, 2011
Last modified: October 29, 2014
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3