O74254 (AMYG_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucoamylase 1
EC=3.2.1.3

Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase

Gene names

Name:	GAM1
Synonyms:	GCA1
ORF Names:	CaO19.12365, CaO19.4899

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	946 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal 1,4-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose. Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.
Subcellular location	Secreted › cell wall. Membrane; Peripheral membrane protein.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the glycosyl hydrolase 31 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Polysaccharide degradation
Cellular component	Membrane Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate catabolic process Inferred from sequence or structural similarity Ref.1. Source: CGD polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW single-species biofilm formation on inanimate substrate Inferred from mutant phenotype PubMed 19529758. Source: CGD
Cellular_component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from direct assay PubMed 20641015. Source: CGD plasma membrane Inferred from sequence or structural similarity Ref.1. Source: CGD
Molecular_function	amylase activity Inferred from sequence or structural similarity Ref.1. Source: CGD carbohydrate binding Inferred from electronic annotation. Source: InterPro glucan 1,4-alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 946	926	Glucoamylase 1		PRO_0000018585
Regions
	Compositional bias	519 – 532	14	Ser/Thr-rich
Sites
	Active site	462	1	By similarity
	Active site	465	1	By similarity
	Active site	628	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	51	1	N-linked (GlcNAc...) Potential
	Glycosylation	68	1	N-linked (GlcNAc...) Potential
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Glycosylation	187	1	N-linked (GlcNAc...) Potential
	Glycosylation	244	1	N-linked (GlcNAc...) Potential
	Glycosylation	373	1	N-linked (GlcNAc...) Potential
	Glycosylation	393	1	N-linked (GlcNAc...) Potential
	Glycosylation	406	1	N-linked (GlcNAc...) Potential
	Glycosylation	437	1	N-linked (GlcNAc...) Potential
	Glycosylation	505	1	N-linked (GlcNAc...) Potential
	Glycosylation	570	1	N-linked (GlcNAc...) Potential
	Glycosylation	772	1	N-linked (GlcNAc...) Potential
	Glycosylation	801	1	N-linked (GlcNAc...) Potential
	Glycosylation	895	1	N-linked (GlcNAc...) Potential
	Glycosylation	912	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	7 – 8	2	VF → FI in allele CaO19.12365.
	Natural variant	102	1	Y → S in allele CaO19.12365.
	Natural variant	113	1	E → D in allele CaO19.12365.
	Natural variant	144	1	N → T in allele CaO19.12365.
	Natural variant	242	1	D → N in allele CaO19.12365.
	Natural variant	248	1	G → A in allele CaO19.12365.
	Natural variant	397	1	D → N in allele CaO19.12365.
	Natural variant	477	1	G → D in allele CaO19.12365.
	Natural variant	544	1	A → G in allele CaO19.12365.
	Natural variant	561	1	G → D in allele CaO19.12365.
	Natural variant	745	1	S → N in allele CaO19.12365.
	Natural variant	752	1	D → E in allele CaO19.12365.
	Natural variant	777	1	I → V in allele CaO19.12365.
	Natural variant	783	1	A → V in allele CaO19.12365.
	Natural variant	826	1	P → A in allele CaO19.12365.
	Natural variant	832	1	E → G in allele CaO19.12365.
Experimental info
	Sequence conflict	529	1	T → A in AAC31968. Ref.1
	Sequence conflict	556	1	N → D in AAC31968. Ref.1
	Sequence conflict	701	1	G → A in AAC31968. Ref.1
	Sequence conflict	704	1	N → K in AAC31968. Ref.1
	Sequence conflict	918	1	N → K in AAC31968. Ref.1
	Sequence conflict	943 – 944	2	SI → TL in AAC31968. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O74254 [UniParc]. Last modified October 19, 2011. Version 2. Checksum: A347712E0F974452	FASTA	946	105,675
	10 20 30 40 50 60 MKLLSKVFVT ALGLTSIVNA APTSSSSAEE AQKTVPVELS IGVKQLPNIH NDSAVDANAV 70 80 90 100 110 120 AKGYSLVNVS LTARGLTGIL KLKEATNIYG YDFEYLNLSV EYQSDTRLNV HIEPTDLTDV 130 140 150 160 170 180 FVLPEELVVK PKLEGDAKTF NFENSDLVFE YDEEDFGFEV LRSSTREVLF STKGNPLVFS 190 200 210 220 230 240 NQFIQFNTTL PKGHSITGLG ESIHGSLNEP GVVKTLYAND IADPIDGNIY GVHPVYYDQR 250 260 270 280 290 300 YDTNTTHGVY WRTSAIQEVV VGETSLTWRA LSGVIDLYFF SGPDPKDVIQ QYVSEIGLPA 310 320 330 340 350 360 MQPYWALGYH QCRWGYDTVE SLETVVENFK KFDIPLETIW SDIDYMDGYK DFTNDPYRFP 370 380 390 400 410 420 TDKFRKFLDD LHNNSQHYVP IFDAAIYVPN PNNATDDDYE PFHLGNESDV FLKNPDGSLY 430 440 450 460 470 480 IGAVWPGYTV FPDFLANNTQ EYWNKMFKDW YERIPFDGIW TDMNEVSSFC VGSCGTGRYF 490 500 510 520 530 540 DNPVHPPFEV GYSGSDYPLG FDKSNASEWK SISEAAAATK TTTTTSSSTS TSIDGKNTLA 550 560 570 580 590 600 PGKANINYPP YAINNNQGDH GLATHAISPN ATHADGTVEY DIHNIYGLIQ ERAIYEALLE 610 620 630 640 650 660 IHPNKRPFII GRSSFAGSGK YMGHWGGDNY ADYYMMYFSI PQALSMGLSG IPFFGVDACG 670 680 690 700 710 720 FNGNTDMELC SRWMQLASFF PFYRNHNVLG AIPQEPYVWE GVMNATKTSI NVRYSLLPYY 730 740 750 760 770 780 YTLLHESHVT GIPIMRAFNW QFPYSKELAG VDTQFFVGDA LLVTPVLEPG VNHTKGIFPG 790 800 810 820 830 840 ENAVYYDFYT HKKQKFTAGK NETLAAPLGH IPLHIKGGNI IPTQEPGYTT TESRKNPFGL 850 860 870 880 890 900 LVALDAEGTA SGKLYLDDGE SVDVEEALYV DFVASKNKLV ASVFGEYEVR QPLANVTILG 910 920 930 940 VDSEPKKVLF NNETVSHNYE NGAVYLTDLE KFTKEGAFAE EFSIQW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and cloning of GCA1, a gene that encodes a cell surface glucoamylase from Candida albicans." Sturtevant J., Dixon F., Wadsworth E., Latge J.-P., Zhao X.-J., Calderone R. Med. Mycol. 37:357-366(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 193-213; 776-792 AND 817-835. Strain: SC5314 / ATCC MYA-2876.
[2]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF082188 Genomic DNA. Translation: AAC31968.1. AACQ01000001 Genomic DNA. Translation: EAL04887.1. AACQ01000002 Genomic DNA. Translation: EAL04694.1.
RefSeq	XP_723393.1. XM_718300.1. XP_723581.1. XM_718488.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0005531.
Protein family/group databases
CAZy	GH31. Glycoside Hydrolase Family 31.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3634903. 3635124.
KEGG	cal:CaO19.12365. cal:CaO19.4899.
Organism-specific databases
CGD	CAL0066397. GCA1.
Phylogenomic databases
eggNOG	COG1501.
KO	K01187.
Family and domain databases
InterPro	IPR011013. Gal_mutarotase_SF_dom. IPR000322. Glyco_hydro_31. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR22762. PTHR22762. 1 hit.
Pfam	PF01055. Glyco_hydro_31. 1 hit. [Graphical view]
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit. PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMYG_CANAL

Accession

Primary (citable) accession number: O74254
Secondary accession number(s): Q5AP64, Q5APQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	October 19, 2011
Last modified:	April 3, 2013
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program