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Protein

Guanine nucleotide-binding protein subunit alpha

Gene

CGA1

Organism
Cochliobolus heterostrophus (strain C4 / ATCC 48331 / race T) (Southern corn leaf blight fungus) (Bipolaris maydis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Involved in the mating pathway.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471MagnesiumBy similarity
Metal bindingi181 – 1811MagnesiumBy similarity
Binding sitei325 – 3251GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi40 – 478GTPBy similarity
Nucleotide bindingi175 – 1817GTPBy similarity
Nucleotide bindingi200 – 2045GTPBy similarity
Nucleotide bindingi269 – 2724GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit alpha
Gene namesi
Name:CGA1
ORF Names:COCC4DRAFT_143328
OrganismiCochliobolus heterostrophus (strain C4 / ATCC 48331 / race T) (Southern corn leaf blight fungus) (Bipolaris maydis)
Taxonomic identifieri665024 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaePleosporaceaeBipolaris
Proteomesi
  • UP000012338 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 353352Guanine nucleotide-binding protein subunit alphaPRO_0000203596Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Lipidationi3 – 31S-palmitoyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Proteomic databases

PRIDEiO74227.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Structurei

3D structure databases

ProteinModelPortaliO74227.
SMRiO74227. Positions 9-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(q) subfamily.Curated

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR002975. Fungi_Gprotein_alpha.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR01241. GPROTEINAFNG.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O74227-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCGMSTEEK EGKQRNEEIE NQLKRDKLMQ RNEIKMLLLG AGESGKSTIL
60 70 80 90 100
KQMKLIHEGG YSRDERESFK EIIFSNTVQS MRVILEAMES LELPLDDQRA
110 120 130 140 150
EYHVQTIFMQ PAQIEGDSLP PEVGNAISVL WKDAGVQQCF QRSREYQLND
160 170 180 190 200
SAKYYFDSID RIAAPDYIPN DQDVLRSRVK TTGITETTFI IGDLTYRMFD
210 220 230 240 250
VGGQRSERKK WIHCFENVTT ILFLVAISEY DQLLFEDETV NRMQEALTLF
260 270 280 290 300
DSICNSRWFV KTSIILFLNK IDRFKEKLPV SPMKNYFPDY EGGPDYAAAC
310 320 330 340 350
DYILNRFVSL NQHETKQIYT HFTCATDTMQ IRFVMAAVND IIIQENLRLC

GLI
Length:353
Mass (Da):40,873
Last modified:January 23, 2007 - v3
Checksum:i6F151E33C0712A1D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF070446 Genomic DNA. Translation: AAC23576.1.
KB733460 Genomic DNA. Translation: ENI03377.1.
RefSeqiXP_014077286.1. XM_014221811.1.

Genome annotation databases

EnsemblFungiiENI03377; ENI03377; COCC4DRAFT_143328.
GeneIDi25838107.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF070446 Genomic DNA. Translation: AAC23576.1.
KB733460 Genomic DNA. Translation: ENI03377.1.
RefSeqiXP_014077286.1. XM_014221811.1.

3D structure databases

ProteinModelPortaliO74227.
SMRiO74227. Positions 9-353.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO74227.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiENI03377; ENI03377; COCC4DRAFT_143328.
GeneIDi25838107.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR002975. Fungi_Gprotein_alpha.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR01241. GPROTEINAFNG.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A G protein alpha subunit from Cochliobolus heterostrophus involved in mating and appressorium formation."
    Horwitz B.A., Sharon A., Lu S.W., Ritter V., Sandrock T.M., Yoder O.C., Turgeon B.G.
    Fungal Genet. Biol. 26:19-32(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C4 / ATCC 48331 / race T.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C4 / ATCC 48331 / race T.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C4 / ATCC 48331 / race T.

Entry informationi

Entry nameiGPA1_COCH4
AccessioniPrimary (citable) accession number: O74227
Secondary accession number(s): N4XDY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 83 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.