Reviewed, UniProtKB/Swiss-Prot O74215 (DAK2_SCHPO)

Names and origin

Protein names

Recommended name:
    Dihydroxyacetone kinase 2
      Short name=DHA kinase 2
    EC=2.7.1.29
Alternative name(s):
    Glycerone kinase 2

Gene names

Name:	dak2
Synonyms:	dak1
ORF Names:	SPAC977.16c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + glycerone = ADP + glycerone phosphate.
Pathway	Polyol metabolism; glycerol fermentation; glycerone phosphate from glycerol (oxidative route): step 2/2.
Miscellaneous	Seems to be missing in strain 972.
Sequence similarities	Belongs to the dihydroxyacetone kinase (DAK) family. Contains 1 DAK1 (dihydroxyacetone kinase subunit 1) domain. Contains 1 DAK2 (dihydroxyacetone kinase subunit 2) domain.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerone kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	Dihydroxyacetone kinase 2		PRO_0000121521
Regions
	Domain	17 – 345	329	DAK1
	Domain	410 – 587	178	DAK2
Experimental info
	Sequence conflict	9	1	D → E in AAC78808. Ref.1
	Sequence conflict	36	1	I → V in AAC83220. Ref.1
	Sequence conflict	48	1	L → P in AAC78808. Ref.1
	Sequence conflict	428	1	T → I in AAC78808. Ref.1
	Sequence conflict	428	1	T → I in AAC83220. Ref.1
	Sequence conflict	476	1	H → R in AAC78808. Ref.1
	Sequence conflict	476	1	H → R in AAC83220. Ref.1
	Sequence conflict	583	1	N → K in AAC78808. Ref.1
	Sequence conflict	583	1	N → K in AAC83220. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O74215-1 [UniParc]. Last modified August 14, 2001. Version 2. Checksum: 7FCCEB2EB5CC1C21	FASTA	591	62,127
	10 20 30 40 50 60 MSVKQFVSDG HIVRPYLLGL ARSNPGLTVI EHDRVIYRTA SAPGSGDLPK VTLVSGGGSG 70 80 90 100 110 120 HEPTHAGFVG DGALDAVACG DIFASPSTKQ IYSALKAVAS PKGTLIIVKN YTGDIIHFGL 130 140 150 160 170 180 AAERAKAAGM NVELVAVGDD VSVGKKRGAL VGRRGLGATV LVHKIAGSAA ALGLDLHQVA 190 200 210 220 230 240 QVAQSVIDNA ATIAASLDHC AVPGRKFETN LGPDEYEIGM GIHNEPGTFK SSPLPSIPEL 250 260 270 280 290 300 VTEMLSILFG EKNPDNSFVE FSSKDDVILL VNNMGGMSNL ELGYATEVVS EQLAKRGIIP 310 320 330 340 350 360 KRTMSGTFVT ALNGPGFGIT LVNASKATPD IFKYFDLPTT ASGWNVSYHN AKDWEVLADG 370 380 390 400 410 420 KVPTAPALEH TRNEKHSGVK ADPKMFTKIL KAAVDAINEF EPKTTWYDTI AGDGDCGTTL 430 440 450 460 470 480 VNGGEAITKA INDKSIRLDD GVNGIDDLAY IVEDSMGGTS GGLYSIYLSA LAKGVHESGD 490 500 510 520 530 540 SELSVHTFAF ASKYALDALF KYTRARKGFR TLIDAIQPFV ETLNEGKGLD AAAKAATEGS 550 560 570 580 590 EQTRKMDAVV GRASYVAKEE LHKLDSEGGL PDPGAFALAA ILNAIVEASE H

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of two genes encoding dihydroxyacetone kinase from Schizosaccharomyces pombe IFO 0354." Kimura T., Takahashi M., Yoshihara K., Furuichi T., Suzuki K., Imai K., Karita S., Sakka K., Ohmiya K. Biochim. Biophys. Acta 1442:361-368(1998) [PubMed: 9804990] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IFO 0354.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed: 10759889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 103-168. Strain: ATCC 38364 / 968.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF059204 Genomic DNA. Translation: AAC78808.1. AF059205 Genomic DNA. Translation: AAC83220.1. CU329670 Genomic DNA. Translation: CAB69638.1. AB027813 Genomic DNA. Translation: BAA87117.1.
PIR	T43702. T43721. T50287.
RefSeq	NP_592787.1.
3D structure databases
HSSP	HSSP built from PDB template 1OI2 based on UniProtKB P76015.
ModBase	Search...
Protein-protein interaction databases
STRING	O74215.
Genome annotation databases
GeneID	2541810.
GenomeReviews	Gene locus dak2 in contig CU329670_GR.
KEGG	spo:SPAC977.16c.
NMPDR	fig|4896.1.peg.2757.
Organism-specific databases
GeneDB_Spombe	SPAC977.16c.
Phylogenomic databases
OMA	LLVNNMG.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000532-MON.
BRENDA	2.7.1.29. 653.
Gene expression databases
ArrayExpress	O74215.
Family and domain databases
InterPro	IPR004006. Dak1. IPR004007. Dak2. IPR012734. DhaK_ATP. [Graphical view]
Pfam	PF02733. Dak1. 1 hit. PF02734. Dak2. 1 hit. [Graphical view]
TIGRFAMs	TIGR02361. dak_ATP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DAK2_SCHPO

Accession

Primary (citable) accession number: O74215
Secondary accession number(s): Q9P7U0, Q9URP4, Q9UU48

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 14, 2001
Last modified:	November 3, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)