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Protein

Dihydroxyacetone kinase 2

Gene

dak2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde.By similarity

Catalytic activityi

ATP + glycerone = ADP + glycerone phosphate.
ATP + D-glyceraldehyde = ADP + D-glyceraldehyde 3-phosphate.

Pathwayi: glycerol fermentation

This protein is involved in step 2 of the subpathway that synthesizes glycerone phosphate from glycerol (oxidative route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glycerol dehydrogenase 1 (gld1)
  2. Dihydroxyacetone kinase 1 (dak1), Dihydroxyacetone kinase 2 (dak2)
This subpathway is part of the pathway glycerol fermentation, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from glycerol (oxidative route), the pathway glycerol fermentation and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei109SubstrateBy similarity1
Binding sitei114SubstrateBy similarity1
Active sitei223Tele-hemiaminal-histidine intermediatePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi413 – 416ATPBy similarity4
Nucleotide bindingi459 – 460ATPBy similarity2
Nucleotide bindingi511 – 512ATPBy similarity2
Nucleotide bindingi572 – 574ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

  • glycerol metabolic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16996.
ReactomeiR-SPO-70350. Fructose catabolism.
UniPathwayiUPA00617; UER00669.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroxyacetone kinase 2 (EC:2.7.1.28, EC:2.7.1.29)
Short name:
DHA kinase 2
Alternative name(s):
Glycerone kinase 2
Triokinase 2
Triose kinase 2
Gene namesi
Name:dak2
Synonyms:dak1
ORF Names:SPAC977.16c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC977.16c.
PomBaseiSPAC977.16c. dak2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001215211 – 591Dihydroxyacetone kinase 2Add BLAST591

Proteomic databases

MaxQBiO74215.
PRIDEiO74215.

Interactioni

Protein-protein interaction databases

BioGridi278301. 1 interactor.
MINTiMINT-4678088.

Structurei

3D structure databases

ProteinModelPortaliO74215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 344DhaKPROSITE-ProRule annotationAdd BLAST337
Domaini384 – 587DhaLPROSITE-ProRule annotationAdd BLAST204

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni58 – 61Substrate bindingBy similarity4

Sequence similaritiesi

Contains 1 DhaK domain.PROSITE-ProRule annotation
Contains 1 DhaL domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000234158.
InParanoidiO74215.
KOiK00863.
OrthoDBiEOG092C1OGC.
PhylomeDBiO74215.

Family and domain databases

InterProiIPR012734. DhaK_ATP.
IPR004006. DhaK_dom.
IPR004007. DhaL_dom.
[Graphical view]
PfamiPF02733. Dak1. 1 hit.
PF02734. Dak2. 1 hit.
[Graphical view]
SMARTiSM01120. Dak2. 1 hit.
[Graphical view]
SUPFAMiSSF101473. SSF101473. 1 hit.
TIGRFAMsiTIGR02361. dak_ATP. 1 hit.
PROSITEiPS51481. DHAK. 1 hit.
PS51480. DHAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74215-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVKQFVSDG HIVRPYLLGL ARSNPGLTVI EHDRVIYRTA SAPGSGDLPK
60 70 80 90 100
VTLVSGGGSG HEPTHAGFVG DGALDAVACG DIFASPSTKQ IYSALKAVAS
110 120 130 140 150
PKGTLIIVKN YTGDIIHFGL AAERAKAAGM NVELVAVGDD VSVGKKRGAL
160 170 180 190 200
VGRRGLGATV LVHKIAGSAA ALGLDLHQVA QVAQSVIDNA ATIAASLDHC
210 220 230 240 250
AVPGRKFETN LGPDEYEIGM GIHNEPGTFK SSPLPSIPEL VTEMLSILFG
260 270 280 290 300
EKNPDNSFVE FSSKDDVILL VNNMGGMSNL ELGYATEVVS EQLAKRGIIP
310 320 330 340 350
KRTMSGTFVT ALNGPGFGIT LVNASKATPD IFKYFDLPTT ASGWNVSYHN
360 370 380 390 400
AKDWEVLADG KVPTAPALEH TRNEKHSGVK ADPKMFTKIL KAAVDAINEF
410 420 430 440 450
EPKTTWYDTI AGDGDCGTTL VNGGEAITKA INDKSIRLDD GVNGIDDLAY
460 470 480 490 500
IVEDSMGGTS GGLYSIYLSA LAKGVHESGD SELSVHTFAF ASKYALDALF
510 520 530 540 550
KYTRARKGFR TLIDAIQPFV ETLNEGKGLD AAAKAATEGS EQTRKMDAVV
560 570 580 590
GRASYVAKEE LHKLDSEGGL PDPGAFALAA ILNAIVEASE H
Length:591
Mass (Da):62,127
Last modified:August 14, 2001 - v2
Checksum:i7FCCEB2EB5CC1C21
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9D → E in AAC78808 (PubMed:9804990).Curated1
Sequence conflicti36I → V in AAC83220 (PubMed:9804990).Curated1
Sequence conflicti48L → P in AAC78808 (PubMed:9804990).Curated1
Sequence conflicti428T → I in AAC78808 (PubMed:9804990).Curated1
Sequence conflicti428T → I in AAC83220 (PubMed:9804990).Curated1
Sequence conflicti476H → R in AAC78808 (PubMed:9804990).Curated1
Sequence conflicti476H → R in AAC83220 (PubMed:9804990).Curated1
Sequence conflicti583N → K in AAC78808 (PubMed:9804990).Curated1
Sequence conflicti583N → K in AAC83220 (PubMed:9804990).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059204 Genomic DNA. Translation: AAC78808.1.
AF059205 Genomic DNA. Translation: AAC83220.1.
CU329670 Genomic DNA. Translation: CAB69638.1.
AB027813 Genomic DNA. Translation: BAA87117.1.
PIRiT43702.
T43721.
T50287.
RefSeqiNP_592787.1. NM_001018187.2.

Genome annotation databases

EnsemblFungiiSPAC977.16c.1; SPAC977.16c.1:pep; SPAC977.16c.
GeneIDi2541810.
KEGGispo:SPAC977.16c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059204 Genomic DNA. Translation: AAC78808.1.
AF059205 Genomic DNA. Translation: AAC83220.1.
CU329670 Genomic DNA. Translation: CAB69638.1.
AB027813 Genomic DNA. Translation: BAA87117.1.
PIRiT43702.
T43721.
T50287.
RefSeqiNP_592787.1. NM_001018187.2.

3D structure databases

ProteinModelPortaliO74215.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278301. 1 interactor.
MINTiMINT-4678088.

Proteomic databases

MaxQBiO74215.
PRIDEiO74215.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC977.16c.1; SPAC977.16c.1:pep; SPAC977.16c.
GeneIDi2541810.
KEGGispo:SPAC977.16c.

Organism-specific databases

EuPathDBiFungiDB:SPAC977.16c.
PomBaseiSPAC977.16c. dak2.

Phylogenomic databases

HOGENOMiHOG000234158.
InParanoidiO74215.
KOiK00863.
OrthoDBiEOG092C1OGC.
PhylomeDBiO74215.

Enzyme and pathway databases

UniPathwayiUPA00617; UER00669.
BioCyciMetaCyc:MONOMER-16996.
ReactomeiR-SPO-70350. Fructose catabolism.

Miscellaneous databases

PROiO74215.

Family and domain databases

InterProiIPR012734. DhaK_ATP.
IPR004006. DhaK_dom.
IPR004007. DhaL_dom.
[Graphical view]
PfamiPF02733. Dak1. 1 hit.
PF02734. Dak2. 1 hit.
[Graphical view]
SMARTiSM01120. Dak2. 1 hit.
[Graphical view]
SUPFAMiSSF101473. SSF101473. 1 hit.
TIGRFAMsiTIGR02361. dak_ATP. 1 hit.
PROSITEiPS51481. DHAK. 1 hit.
PS51480. DHAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDAK2_SCHPO
AccessioniPrimary (citable) accession number: O74215
Secondary accession number(s): Q9P7U0, Q9URP4, Q9UU48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 14, 2001
Last modified: November 30, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Seems to be missing in strain 972.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.