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Protein

Dihydroxyacetone kinase 2

Gene

dak2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde.By similarity

Miscellaneous

Seems to be missing in strain 972.

Catalytic activityi

ATP + glycerone = ADP + glycerone phosphate.
ATP + D-glyceraldehyde = ADP + D-glyceraldehyde 3-phosphate.

Pathwayi: glycerol fermentation

This protein is involved in step 2 of the subpathway that synthesizes glycerone phosphate from glycerol (oxidative route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glycerol dehydrogenase 1 (gld1)
  2. Dihydroxyacetone kinase 1 (dak1), Dihydroxyacetone kinase 2 (dak2)
This subpathway is part of the pathway glycerol fermentation, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from glycerol (oxidative route), the pathway glycerol fermentation and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei109SubstrateBy similarity1
Binding sitei114SubstrateBy similarity1
Active sitei223Tele-hemiaminal-histidine intermediatePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi413 – 416ATPBy similarity4
Nucleotide bindingi459 – 460ATPBy similarity2
Nucleotide bindingi511 – 512ATPBy similarity2
Nucleotide bindingi572 – 574ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

  • glycerol metabolic process Source: PomBase

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycerol metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16996
ReactomeiR-SPO-70350 Fructose catabolism
UniPathwayiUPA00617; UER00669

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroxyacetone kinase 2 (EC:2.7.1.28, EC:2.7.1.29)
Short name:
DHA kinase 2
Alternative name(s):
Glycerone kinase 2
Triokinase 2
Triose kinase 2
Gene namesi
Name:dak2
Synonyms:dak1
ORF Names:SPAC977.16c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC977.16c
PomBaseiSPAC977.16c dak2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001215211 – 591Dihydroxyacetone kinase 2Add BLAST591

Proteomic databases

MaxQBiO74215
PaxDbiO74215
PRIDEiO74215

PTM databases

iPTMnetiO74215

Interactioni

Protein-protein interaction databases

BioGridi278301, 1 interactor
STRINGi4896.SPAC977.16c.1

Structurei

3D structure databases

ProteinModelPortaliO74215
SMRiO74215
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 344DhaKPROSITE-ProRule annotationAdd BLAST337
Domaini384 – 587DhaLPROSITE-ProRule annotationAdd BLAST204

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni58 – 61Substrate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000234158
InParanoidiO74215
KOiK00863
OMAiSTKGMEA
OrthoDBiEOG092C1OGC
PhylomeDBiO74215

Family and domain databases

Gene3Di1.25.40.340, 1 hit
InterProiView protein in InterPro
IPR012734 DhaK_ATP
IPR004006 DhaK_dom
IPR004007 DhaL_dom
IPR036117 DhaL_dom_sf
PfamiView protein in Pfam
PF02733 Dak1, 1 hit
PF02734 Dak2, 1 hit
SMARTiView protein in SMART
SM01120 Dak2, 1 hit
SUPFAMiSSF101473 SSF101473, 1 hit
TIGRFAMsiTIGR02361 dak_ATP, 1 hit
PROSITEiView protein in PROSITE
PS51481 DHAK, 1 hit
PS51480 DHAL, 1 hit

Sequencei

Sequence statusi: Complete.

O74215-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVKQFVSDG HIVRPYLLGL ARSNPGLTVI EHDRVIYRTA SAPGSGDLPK
60 70 80 90 100
VTLVSGGGSG HEPTHAGFVG DGALDAVACG DIFASPSTKQ IYSALKAVAS
110 120 130 140 150
PKGTLIIVKN YTGDIIHFGL AAERAKAAGM NVELVAVGDD VSVGKKRGAL
160 170 180 190 200
VGRRGLGATV LVHKIAGSAA ALGLDLHQVA QVAQSVIDNA ATIAASLDHC
210 220 230 240 250
AVPGRKFETN LGPDEYEIGM GIHNEPGTFK SSPLPSIPEL VTEMLSILFG
260 270 280 290 300
EKNPDNSFVE FSSKDDVILL VNNMGGMSNL ELGYATEVVS EQLAKRGIIP
310 320 330 340 350
KRTMSGTFVT ALNGPGFGIT LVNASKATPD IFKYFDLPTT ASGWNVSYHN
360 370 380 390 400
AKDWEVLADG KVPTAPALEH TRNEKHSGVK ADPKMFTKIL KAAVDAINEF
410 420 430 440 450
EPKTTWYDTI AGDGDCGTTL VNGGEAITKA INDKSIRLDD GVNGIDDLAY
460 470 480 490 500
IVEDSMGGTS GGLYSIYLSA LAKGVHESGD SELSVHTFAF ASKYALDALF
510 520 530 540 550
KYTRARKGFR TLIDAIQPFV ETLNEGKGLD AAAKAATEGS EQTRKMDAVV
560 570 580 590
GRASYVAKEE LHKLDSEGGL PDPGAFALAA ILNAIVEASE H
Length:591
Mass (Da):62,127
Last modified:August 14, 2001 - v2
Checksum:i7FCCEB2EB5CC1C21
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9D → E in AAC78808 (PubMed:9804990).Curated1
Sequence conflicti36I → V in AAC83220 (PubMed:9804990).Curated1
Sequence conflicti48L → P in AAC78808 (PubMed:9804990).Curated1
Sequence conflicti428T → I in AAC78808 (PubMed:9804990).Curated1
Sequence conflicti428T → I in AAC83220 (PubMed:9804990).Curated1
Sequence conflicti476H → R in AAC78808 (PubMed:9804990).Curated1
Sequence conflicti476H → R in AAC83220 (PubMed:9804990).Curated1
Sequence conflicti583N → K in AAC78808 (PubMed:9804990).Curated1
Sequence conflicti583N → K in AAC83220 (PubMed:9804990).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059204 Genomic DNA Translation: AAC78808.1
AF059205 Genomic DNA Translation: AAC83220.1
CU329670 Genomic DNA Translation: CAB69638.1
AB027813 Genomic DNA Translation: BAA87117.1
PIRiT43702
T43721
T50287
RefSeqiNP_592787.1, NM_001018187.2

Genome annotation databases

EnsemblFungiiSPAC977.16c.1; SPAC977.16c.1:pep; SPAC977.16c
GeneIDi2541810
KEGGispo:SPAC977.16c

Similar proteinsi

Entry informationi

Entry nameiDAK2_SCHPO
AccessioniPrimary (citable) accession number: O74215
Secondary accession number(s): Q9P7U0, Q9URP4, Q9UU48
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 14, 2001
Last modified: March 28, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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