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Protein

Endopolygalacturonase I

Gene

pgaI

Organism
Aspergillus aculeatus
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall.

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei219Proton donor1
Active sitei2411

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Endopolygalacturonase I (EC:3.2.1.15)
Alternative name(s):
Pectinase 1
Polygalacturonase I
Short name:
PG-I
Gene namesi
Name:pgaI
Synonyms:pg1, pga1
OrganismiAspergillus aculeatus
Taxonomic identifieri5053 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000002476221 – 39Sequence analysisAdd BLAST19
ChainiPRO_000002476340 – 378Endopolygalacturonase IAdd BLAST339

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi43 ↔ 61
Glycosylationi44O-linked (Man...)1 Publication1
Glycosylationi46O-linked (Man...)1 Publication1
Glycosylationi48O-linked (Man...)1 Publication1
Glycosylationi52O-linked (Man...)1 Publication1
Glycosylationi53O-linked (Man...)1 Publication1
Glycosylationi55O-linked (Man...)1 Publication1
Glycosylationi57O-linked (Man...)1 Publication1
Glycosylationi62O-linked (Man...)1 Publication1
Glycosylationi63O-linked (Man...)1 Publication1
Glycosylationi73O-linked (Man...)1 Publication1
Disulfide bondi221 ↔ 237
Glycosylationi258N-linked (GlcNAc...)1 Publication1
Disulfide bondi345 ↔ 350
Disulfide bondi369 ↔ 378

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Structurei

Secondary structure

1378
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi42 – 46Combined sources5
Helixi47 – 49Combined sources3
Helixi50 – 57Combined sources8
Helixi58 – 60Combined sources3
Beta strandi62 – 68Combined sources7
Beta strandi77 – 79Combined sources3
Beta strandi87 – 96Combined sources10
Beta strandi105 – 112Combined sources8
Beta strandi114 – 117Combined sources4
Beta strandi122 – 124Combined sources3
Helixi127 – 129Combined sources3
Turni135 – 137Combined sources3
Beta strandi138 – 140Combined sources3
Beta strandi146 – 158Combined sources13
Beta strandi160 – 162Combined sources3
Beta strandi169 – 173Combined sources5
Beta strandi175 – 181Combined sources7
Beta strandi183 – 185Combined sources3
Helixi187 – 189Combined sources3
Turni190 – 193Combined sources4
Beta strandi199 – 204Combined sources6
Beta strandi206 – 212Combined sources7
Beta strandi214 – 216Combined sources3
Beta strandi221 – 234Combined sources14
Beta strandi236 – 241Combined sources6
Beta strandi243 – 248Combined sources6
Beta strandi250 – 252Combined sources3
Beta strandi255 – 268Combined sources14
Beta strandi270 – 278Combined sources9
Beta strandi284 – 309Combined sources26
Beta strandi318 – 320Combined sources3
Beta strandi322 – 334Combined sources13
Beta strandi338 – 344Combined sources7
Beta strandi350 – 364Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IA5X-ray2.00A40-378[»]
1IB4X-ray2.00A/B40-378[»]
ProteinModelPortaliO74213.
SMRiO74213.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO74213.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati174 – 204PbH1 1Add BLAST31
Repeati205 – 226PbH1 2Add BLAST22
Repeati227 – 247PbH1 3Add BLAST21
Repeati256 – 277PbH1 4Add BLAST22
Repeati285 – 307PbH1 5Add BLAST23

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 5 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O74213-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLNTTLLVS LALGAASVLA SPAPPAITAP PTAEEIAKRA TTCTFSGSNG
60 70 80 90 100
ASSASKSKTS CSTIVLSNVA VPSGTTLDLT KLNDGTHVIF SGETTFGYKE
110 120 130 140 150
WSGPLISVSG SDLTITGASG HSINGDGSRW WDGEGGNGGK TKPKFFAAHS
160 170 180 190 200
LTNSVISGLK IVNSPVQVFS VAGSDYLTLK DITIDNSDGD DNGGHNTDAF
210 220 230 240 250
DIGTSTYVTI SGATVYNQDD CVAVNSGENI YFSGGYCSGG HGLSIGSVGG
260 270 280 290 300
RSDNTVKNVT FVDSTIINSD NGVRIKTNID TTGSVSDVTY KDITLTSIAK
310 320 330 340 350
YGIVVQQNYG DTSSTPTTGV PITDFVLDNV HGSVVSSGTN ILISCGSGSC
360 370
SDWTWTDVSV SGGKTSSKCT NVPSGASC
Length:378
Mass (Da):38,528
Last modified:November 1, 1998 - v1
Checksum:iDC5B9B6E1D2059C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF054893 mRNA. Translation: AAC23565.1.
AJ581480 mRNA. Translation: CAE46193.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF054893 mRNA. Translation: AAC23565.1.
AJ581480 mRNA. Translation: CAE46193.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IA5X-ray2.00A40-378[»]
1IB4X-ray2.00A/B40-378[»]
ProteinModelPortaliO74213.
SMRiO74213.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiO74213.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGLR1_ASPAC
AccessioniPrimary (citable) accession number: O74213
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.