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O74212 (FAD5_MORAP) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Delta(5) fatty acid desaturase

Short name=Delta-5 fatty acid desaturase
EC=1.14.19.-
Gene names
Name:DES1
OrganismMortierella alpina (Mortierella renispora)
Taxonomic identifier64518 [NCBI]
Taxonomic lineageEukaryotaFungiFungi incertae sedisBasal fungal lineagesMucoromycotinaMortierellalesMortierellaceaeMortierella

Protein attributes

Sequence length446 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the conversion of di-homo-Delta-linolenic acid to arachidonic acid. Essential in the production of eicosanoids.

Cofactor

Iron By similarity.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Domain

The histidine box domains may contain the active site and/or be involved in metal ion binding.

Sequence similarities

Belongs to the fatty acid desaturase family.

Contains 1 cytochrome b5 heme-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 446446Delta(5) fatty acid desaturase
PRO_0000185409

Regions

Transmembrane125 – 14521Helical; Potential
Transmembrane150 – 17021Helical; Potential
Domain6 – 8277Cytochrome b5 heme-binding
Motif171 – 1755Histidine box-1
Motif207 – 2126Histidine box-2
Motif387 – 3915Histidine box-3

Sites

Metal binding411Iron (heme axial ligand) By similarity
Metal binding641Iron (heme axial ligand) By similarity

Natural variations

Natural variant211G → D in strain: ATCC 32221.
Natural variant881V → I in strain: ATCC 32221.
Natural variant1111D → N in strain: ATCC 32221.
Natural variant2271F → S in strain: ATCC 32221.
Natural variant2481D → H in strain: ATCC 32221.

Sequences

Sequence LengthMass (Da)Tools
O74212 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 8395741C5A3CC9A2

FASTA44651,288
        10         20         30         40         50         60 
MGTDQGKTFT WEELAAHNTK GDLFLAIRGR VYDVTKFLSR HPGGVDTLLL GAGRDVTPVF 

        70         80         90        100        110        120 
EMYHAFGAAD AIMKKYYVGT LVSNELPVFP EPTVFHKTIK TRVEGYFTDR DIDPKNRPEI 

       130        140        150        160        170        180 
WGRYALIFGS LIASYYAQLF VPFVVERTWL QVVFAIIMGF ACAQVGLNPL HDASHFSVTH 

       190        200        210        220        230        240 
NPTVWKILGA THDFFNGASY LVWMYQHMLG HHPYTNIAGA DPDVSTFEPD VRRIKPNQKW 

       250        260        270        280        290        300 
FVNHINQDMF VPFLYGLLAF KVRIQDINIL YFVKTNDAIR VNPISTWHTV MFWGGKAFFV 

       310        320        330        340        350        360 
WYRLIVPLQY LPLGKVLLLF TVADMVSSYW LALTFQANHV VEEVQWPLPD ENGIIQKDWA 

       370        380        390        400        410        420 
AMQVETTQDY AHDSHLWTSI TGSLNYQAVH HLFPNVSQHH YPDILAIIKN TCSEYKVPYL 

       430        440 
VKDTFWQAFA SHLEHLRVLG LRPKEE 

« Hide

References

[1]"Isolation of a delta5-fatty acid desaturase gene from Mortierella alpina."
Michaelson L.V., Lazarus C.M., Griffiths G., Napier J.A., Stobart A.K.
J. Biol. Chem. 273:19055-19059(1998) [PubMed: 9668087] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: CBS 210.32 / CCRC 32738.
[2]"Identification of delta5-desaturase from Mortierella alpina by heterologous expression in baker's yeast and canola."
Knutzon D.S., Thurmond J.M., Huang Y.-S., Chaudhary S., Bobik E.G. Jr., Chan G.M., Kirchner S.J., Mukerji P.
J. Biol. Chem. 273:29360-29366(1998) [PubMed: 9792636] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ATCC 32221 / CBS 527.72 / M135.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF054824 mRNA. Translation: AAC39508.1.
AF067654 mRNA. Translation: AAC72755.1.

3D structure databases

ProteinModelPortalO74212.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001199. Cyt_B5.
IPR018506. Cyt_B5_heme-BS.
IPR012171. Fatty_acid/sphinglp_desaturase.
IPR005804. Fatty_acid_desaturase-1.
[Graphical view]
Gene3DG3DSA:3.10.120.10. Cyt_B5. 1 hit.
PfamPF00173. Cyt-b5. 1 hit.
PF00487. FA_desaturase. 1 hit.
[Graphical view]
PIRSFPIRSF015921. FA_sphinglp_des. 1 hit.
PRINTSPR00363. CYTOCHROMEB5.
SUPFAMSSF55856. Cyt_B5. 1 hit.
PROSITEPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFAD5_MORAP
AccessionPrimary (citable) accession number: O74212
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 1, 1998
Last modified: September 21, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families