Reviewed, UniProtKB/Swiss-Prot O74212 (FAD5_MORAP)

Names and origin

Protein names

Recommended name:
    Delta(5) fatty acid desaturase
    EC=1.14.19.-
Alternative name(s):
    Delta-5 fatty acid desaturase

Gene names

Name:

DES1

Organism

Mortierella alpina (Mortierella renispora)

Taxonomic identifier

64518 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Fungi incertae sedis › Basal fungal lineages › Mucoromycotina › Mortierellales › Mortierellaceae › Mortierella

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the conversion of di-homo-Delta-linolenic acid to arachidonic acid. Essential in the production of eicosanoids.
Cofactor	Iron By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Domain	The histidine box domains may contain the active site and/or be involved in metal ion binding.
Sequence similarities	Belongs to the fatty acid desaturase family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Fatty acid biosynthesis Lipid synthesis Transport
Cellular component	Membrane
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Delta(5) fatty acid desaturase		PRO_0000185409
Regions
	Transmembrane	125 – 145	21	Potential
	Transmembrane	150 – 170	21	Potential
	Domain	6 – 82	77	Cytochrome b5 heme-binding
	Motif	171 – 175	5	Histidine box-1
	Motif	207 – 212	6	Histidine box-2
	Motif	387 – 391	5	Histidine box-3
Sites
	Metal binding	41	1	Iron (heme axial ligand) By similarity
	Metal binding	64	1	Iron (heme axial ligand) By similarity
Natural variations
	Natural variant	21	1	G → D in strain: ATCC 32221.
	Natural variant	88	1	V → I in strain: ATCC 32221.
	Natural variant	111	1	D → N in strain: ATCC 32221.
	Natural variant	227	1	F → S in strain: ATCC 32221.
	Natural variant	248	1	D → H in strain: ATCC 32221.

Sequences

	Sequence		Length	Mass (Da)
	O74212-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 8395741C5A3CC9A2	FASTA	446	51,288
	10 20 30 40 50 60 MGTDQGKTFT WEELAAHNTK GDLFLAIRGR VYDVTKFLSR HPGGVDTLLL GAGRDVTPVF 70 80 90 100 110 120 EMYHAFGAAD AIMKKYYVGT LVSNELPVFP EPTVFHKTIK TRVEGYFTDR DIDPKNRPEI 130 140 150 160 170 180 WGRYALIFGS LIASYYAQLF VPFVVERTWL QVVFAIIMGF ACAQVGLNPL HDASHFSVTH 190 200 210 220 230 240 NPTVWKILGA THDFFNGASY LVWMYQHMLG HHPYTNIAGA DPDVSTFEPD VRRIKPNQKW 250 260 270 280 290 300 FVNHINQDMF VPFLYGLLAF KVRIQDINIL YFVKTNDAIR VNPISTWHTV MFWGGKAFFV 310 320 330 340 350 360 WYRLIVPLQY LPLGKVLLLF TVADMVSSYW LALTFQANHV VEEVQWPLPD ENGIIQKDWA 370 380 390 400 410 420 AMQVETTQDY AHDSHLWTSI TGSLNYQAVH HLFPNVSQHH YPDILAIIKN TCSEYKVPYL 430 440 VKDTFWQAFA SHLEHLRVLG LRPKEE

References

[1]	"Isolation of a delta5-fatty acid desaturase gene from Mortierella alpina." Michaelson L.V., Lazarus C.M., Griffiths G., Napier J.A., Stobart A.K. J. Biol. Chem. 273:19055-19059(1998) [PubMed: 9668087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CBS 210.32 / CCRC 32738.
[2]	"Identification of delta5-desaturase from Mortierella alpina by heterologous expression in baker's yeast and canola." Knutzon D.S., Thurmond J.M., Huang Y.-S., Chaudhary S., Bobik E.G. Jr., Chan G.M., Kirchner S.J., Mukerji P. J. Biol. Chem. 273:29360-29366(1998) [PubMed: 9792636] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 32221 / CBS 527.72 / M135.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF054824 mRNA. Translation: AAC39508.1. AF067654 mRNA. Translation: AAC72755.1.
3D structure databases
HSSP	HSSP built from PDB template 1EUE based on UniProtKB P04166.
ModBase	Search...
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. IPR012171. Fatty_acid/sphinglp_desaturase. IPR005804. Fatty_acid_desaturase-1. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. PF00487. FA_desaturase. 1 hit. [Graphical view]
PIRSF	PIRSF015921. FA_sphinglp_des. 1 hit.
PRINTS	PR00363. CYTOCHROMEB5.
ProDom	PD000612. Cyt_B5. 1 hit. PD001081. FA_desat_sub. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FAD5_MORAP

Accession

Primary (citable) accession number: O74212

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)