Reviewed,
UniProtKB/Swiss-Prot O74212 (FAD5_MORAP)
Last modified
June 16, 2009.
Version 50.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Delta(5) fatty acid desaturase EC=1.14.19.- Alternative name(s): Delta-5 fatty acid desaturase | ||
| Gene names |
| ||
| Organism | Mortierella alpina (Mortierella renispora) | ||
| Taxonomic identifier | 64518 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Fungi › Fungi incertae sedis › Basal fungal lineages › Mucoromycotina › Mortierellales › Mortierellaceae › Mortierella |
Protein attributes
| Sequence length | 446 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Involved in the conversion of di-homo-Delta-linolenic acid to arachidonic acid. Essential in the production of eicosanoids. |
| Cofactor | Iron By similarity. |
| Subcellular location | Membrane; Multi-pass membrane protein Potential. |
| Domain | The histidine box domains may contain the active site and/or be involved in metal ion binding. |
| Sequence similarities | Belongs to the fatty acid desaturase family. Contains 1 cytochrome b5 heme-binding domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Electron transport Fatty acid biosynthesis Lipid synthesis Transport |
| Cellular component | Membrane |
| Domain | Transmembrane |
| Ligand | Heme Iron Metal-binding |
| Molecular function | Oxidoreductase |
| Gene Ontology (GO) | |
| Biological process | electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW fatty acid biosynthetic processInferred from electronic annotation. Source: UniProtKB-KW transportInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | heme binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of waterInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 446 | 446 | Delta(5) fatty acid desaturase | PRO_0000185409 | |||||
Regions | |||||||||
| Transmembrane | 125 – 145 | 21 | Potential | ||||||
| Transmembrane | 150 – 170 | 21 | Potential | ||||||
| Domain | 6 – 82 | 77 | Cytochrome b5 heme-binding | ||||||
| Motif | 171 – 175 | 5 | Histidine box-1 | ||||||
| Motif | 207 – 212 | 6 | Histidine box-2 | ||||||
| Motif | 387 – 391 | 5 | Histidine box-3 | ||||||
Sites | |||||||||
| Metal binding | 41 | 1 | Iron (heme axial ligand) By similarity | ||||||
| Metal binding | 64 | 1 | Iron (heme axial ligand) By similarity | ||||||
Natural variations | |||||||||
| Natural variant | 21 | 1 | G → D in strain: ATCC 32221. | ||||||
| Natural variant | 88 | 1 | V → I in strain: ATCC 32221. | ||||||
| Natural variant | 111 | 1 | D → N in strain: ATCC 32221. | ||||||
| Natural variant | 227 | 1 | F → S in strain: ATCC 32221. | ||||||
| Natural variant | 248 | 1 | D → H in strain: ATCC 32221. | ||||||
Sequences
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References
| [1] | "Isolation of a delta5-fatty acid desaturase gene from Mortierella alpina." Michaelson L.V., Lazarus C.M., Griffiths G., Napier J.A., Stobart A.K. J. Biol. Chem. 273:19055-19059(1998) [PubMed: 9668087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CBS 210.32 / CCRC 32738. |
| [2] | "Identification of delta5-desaturase from Mortierella alpina by heterologous expression in baker's yeast and canola." Knutzon D.S., Thurmond J.M., Huang Y.-S., Chaudhary S., Bobik E.G. Jr., Chan G.M., Kirchner S.J., Mukerji P. J. Biol. Chem. 273:29360-29366(1998) [PubMed: 9792636] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 32221 / CBS 527.72 / M135. |
Cross-references
Sequence databases | |
|---|---|
| AF054824 mRNA. Translation: AAC39508.1. AF067654 mRNA. Translation: AAC72755.1. | |
3D structure databases | |
| HSSP | HSSP built from PDB template 1EUE based on UniProtKB P04166. |
| ModBase | Search... |
Family and domain databases | |
| InterPro | IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. IPR012171. Fatty_acid/sphinglp_desaturase. IPR005804. Fatty_acid_desaturase-1. [Graphical view] |
| Gene3D | G3DSA:3.10.120.10. Cyt_B5. 1 hit. |
| Pfam | PF00173. Cyt-b5. 1 hit. PF00487. FA_desaturase. 1 hit. [Graphical view] |
| PIRSF | PIRSF015921. FA_sphinglp_des. 1 hit. |
| PRINTS | PR00363. CYTOCHROMEB5. |
| ProDom | PD000612. Cyt_B5. 1 hit. PD001081. FA_desat_sub. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| PROSITE | PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | FAD5_MORAP | ||||||||
| Accession | Primary (citable) accession number: O74212 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||

Clusters with


