Reviewed, UniProtKB/Swiss-Prot O74201 (UBC2_CANAL)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 2
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase UBC2
    Ubiquitin carrier protein UBC2
    Radiation sensitivity protein 6

Gene names

Name:	UBC2
Synonyms:	RAD6
ORF Names:	CaO19.7195

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. Also involved in postreplication repair of UV-damaged DNA, in N-end rule-dependent protein degradation and in sporulation By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	CytoplasmBy similarity. NucleusBy similarity.
Induction	Up-regulated by UV radiation, heat shock, osmotic stress and nitrogen starvation.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.
Sequence caution	The sequence EAK96213.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	DNA damage DNA repair Sporulation Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Molecular function	Chromatin regulator Ligase
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	Ubiquitin-conjugating enzyme E2 2		PRO_0000082528
Regions
	Region	151 – 179	29	Acidic tail
Sites
	Active site	88	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O74201-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 24A081B5F8E4D316	FASTA	179	20,576
	10 20 30 40 50 60 MSTPARRRLM RDFKRMQQDP PSGVSASPLP DNVMKWNAVI IGPSDTPFED GTFRLLLSFD 70 80 90 100 110 120 EQYPNKPPQV KFISEMFHPN VYASGELCLD ILQNRWSPTY DVSSILTSVQ SLLNDPNISS 130 140 150 160 170 PANVEAANLY KDHRSLYVKR VRETVENSWN DDDDEEEEEE DEDEAEDEDD DDDDNIDED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterisation of CaRAD6 gene from Candida albicans." Leng P., Brown A.J.P. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 3153A.
[2]	"Rad6p represses yeast-hypha morphogenesis in the human fungal pathogen Candida albicans." Leng P., Sudbery P.E., Brown A.J.P. Mol. Microbiol. 35:1264-1275(2000) [PubMed: 10712706] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION. Strain: 3153A.
[3]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF036707 mRNA. Translation: AAC24765.1. AF118145 Genomic DNA. Translation: AAD45241.1. AACQ01000089 Genomic DNA. Translation: EAK96213.1. Sequence problems.
RefSeq	XP_715243.1.
3D structure databases
HSSP	HSSP built from PDB template 1AYZ based on UniProtKB P06104.
SMR	O74201. Positions 2-154.
ModBase	Search...
Genome annotation databases
GeneID	3643143.
KEGG	cal:CaO19.7195.
Organism-specific databases
CGD	CAL0003164. RAD6.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
PANTHER	PTHR11621. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC2_CANAL

Accession

Primary (citable) accession number: O74201
Secondary accession number(s): Q5A0A0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1998
Last modified:	November 25, 2008
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)