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O74201 (UBC2_CANAL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 2

EC=6.3.2.19
Alternative name(s):
Radiation sensitivity protein 6
Ubiquitin carrier protein UBC2
Ubiquitin-protein ligase UBC2
Gene names
Name:UBC2
Synonyms:RAD6
ORF Names:CaO19.7195
OrganismCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]
Taxonomic identifier237561 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

Protein attributes

Sequence length179 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins. Plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. Also involved in postreplication repair of UV-damaged DNA, in N-end rule-dependent protein degradation and in sporulation By similarity. Ref.2

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Induction

Up-regulated by UV radiation, heat shock, osmotic stress and nitrogen starvation. Ref.2

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Sequence caution

The sequence EAK96213.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
Sporulation
Transcription
Transcription regulation
Ubl conjugation pathway
   Cellular componentCytoplasm
Nucleus
   LigandATP-binding
Nucleotide-binding
   Molecular functionChromatin regulator
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

cellular response to DNA damage stimulus

Inferred from expression pattern Ref.2. Source: CGD

cellular response to UV

Inferred from mutant phenotype Ref.2. Source: CGD

chromatin modification

Inferred from electronic annotation. Source: UniProtKB-KW

filamentous growth

Inferred from mutant phenotype Ref.2. Source: CGD

protein autoubiquitination

Inferred from sequence orthology PubMed 16978390. Source: CGD

protein import into peroxisome matrix, receptor recycling

Inferred from sequence orthology PubMed 16978390. Source: CGD

protein monoubiquitination

Inferred from sequence orthology PubMed 16978390. Source: CGD

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

sporulation resulting in formation of a cellular spore

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

peroxisome

Inferred from sequence orthology PubMed 16978390. Source: CGD

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-protein transferase activity

Inferred from genetic interaction Ref.2. Source: CGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 179179Ubiquitin-conjugating enzyme E2 2
PRO_0000082528

Regions

Region151 – 17929Acidic tail

Sites

Active site881Glycyl thioester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
O74201 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 24A081B5F8E4D316

FASTA17920,576
        10         20         30         40         50         60 
MSTPARRRLM RDFKRMQQDP PSGVSASPLP DNVMKWNAVI IGPSDTPFED GTFRLLLSFD 

        70         80         90        100        110        120 
EQYPNKPPQV KFISEMFHPN VYASGELCLD ILQNRWSPTY DVSSILTSVQ SLLNDPNISS 

       130        140        150        160        170 
PANVEAANLY KDHRSLYVKR VRETVENSWN DDDDEEEEEE DEDEAEDEDD DDDDNIDED 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and characterisation of CaRAD6 gene from Candida albicans."
Leng P., Brown A.J.P.
Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 3153A.
[2]"Rad6p represses yeast-hypha morphogenesis in the human fungal pathogen Candida albicans."
Leng P., Sudbery P.E., Brown A.J.P.
Mol. Microbiol. 35:1264-1275(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
Strain: 3153A.
[3]"The diploid genome sequence of Candida albicans."
Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S.
Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF036707 mRNA. Translation: AAC24765.1.
AF118145 Genomic DNA. Translation: AAD45241.1.
AACQ01000089 Genomic DNA. Translation: EAK96213.1. Sequence problems.
RefSeqXP_715243.1. XM_710150.1.
XP_888992.1. XM_883899.1.

3D structure databases

ProteinModelPortalO74201.
SMRO74201. Positions 2-154.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5476.CAL0003164.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3643143.
3703919.
KEGGcal:CaO19.7195.
cal:CaO19_7195.

Organism-specific databases

CGDCAL0003164. RAD6.

Phylogenomic databases

eggNOGCOG5078.
OrthoDBEOG7SBP18.

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBC2_CANAL
AccessionPrimary (citable) accession number: O74201
Secondary accession number(s): Q5A0A0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: May 14, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Candida albicans

Candida albicans: entries and gene names