O74198 (ERG6_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sterol 24-C-methyltransferase
EC=2.1.1.41

Alternative name(s):
Delta(24)-sterol C-methyltransferase

Gene names

Name:	ERG6
ORF Names:	CaO19.1631, CaO19.9199

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol.
Catalytic activity	S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.
Pathway	Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 1/5.
Sequence similarities	Belongs to the class I-like SAM-binding methyltransferase superfamily. ERG6 family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Steroid biosynthesis Steroid metabolism Sterol biosynthesis Sterol metabolism
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to drug Inferred from mutant phenotype PubMed 17604452. Source: CGD ergosterol biosynthetic process Inferred from genetic interaction Ref.1. Source: CGD
Cellular_component	plasma membrane Inferred from direct assay PubMed 19824013. Source: CGD
Molecular_function	sterol 24-C-methyltransferase activity Inferred from direct assay PubMed 20946868. Source: CGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Sterol 24-C-methyltransferase		PRO_0000124789

Sequences

	Sequence		Length	Mass (Da)
	O74198 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 369D094AFCDED0A0	FASTA	376	43,086
	10 20 30 40 50 60 MSPVQLAEKN YERDEQFTKA LHGESYKKTG LSALIAKSKD AASVAAEGYF KHWDGGISKD 70 80 90 100 110 120 DEEKRLNDYS QLTHHYYNLV TDFYEYGWGS SFHFSRYYKG EAFRQATARH EHFLAHKMNL 130 140 150 160 170 180 NENMKVLDVG CGVGGPGREI TRFTDCEIVG LNNNDYQIER ANHYAKKYHL DHKLSYVKGD 190 200 210 220 230 240 FMQMDFEPES FDAVYAIEAT VHAPVLEGVY SEIYKVLKPG GIFGVYEWVM TDKYDETNEE 250 260 270 280 290 300 HRKIAYGIEV GDGIPKMYSR KVAEQALKNV GFEIEYQKDL ADVDDEIPWY YPLSGDLKFC 310 320 330 340 350 360 QTFGDYLTVF RTSRIGRFIT TESVGLMEKI GLAPKGSKQV THALEDAAVN LVEGGRQKLF 370 TPMMLYVVRK PLEKKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing, disruption, and characterization of the Candida albicans sterol methyltransferase (ERG6) gene: drug susceptibility studies in erg6 mutants." Jensen-Pergakes K.L., Kennedy M.A., Lees N.D., Barbuch R., Koegel C., Bard M. Antimicrob. Agents Chemother. 42:1160-1167(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF031941 Genomic DNA. Translation: AAC26626.1. AACQ01000014 Genomic DNA. Translation: EAL02792.1. AACQ01000013 Genomic DNA. Translation: EAL02920.1.
RefSeq	XP_721588.1. XM_716495.1. XP_721708.1. XM_716615.1.
3D structure databases
ProteinModelPortal	O74198.
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0006397.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3636655. 3636783.
KEGG	cal:CaO19.1631. cal:CaO19.9199.
Organism-specific databases
CGD	CAL0006397. ERG6.
Phylogenomic databases
eggNOG	COG0500.
KO	K00559.
Enzyme and pathway databases
UniPathway	UPA00768; UER00760.
Family and domain databases
InterPro	IPR025810. ERG6. IPR013216. Methyltransf_11. IPR013705. Sterol_MeTrfase_C. [Graphical view]
Pfam	PF08241. Methyltransf_11. 1 hit. PF08498. Sterol_MT_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERG6_CANAL

Accession

Primary (citable) accession number: O74198
Secondary accession number(s): Q5AJG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program