Reviewed, UniProtKB/Swiss-Prot O74197 (PUR6_CANGA)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:	ADE2
Ordered Locus Names:	CAGL0K10340g

Organism

Candida glabrata (Yeast) (Torulopsis glabrata) [Complete proteome]

Taxonomic identifier

5478 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Nakaseomyces › mitosporic Nakaseomyces

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Sequence similarities	In the C-terminal section; belongs to the AIR carboxylase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 570	570	Phosphoribosylaminoimidazole carboxylase		PRO_0000075022
Regions
	Domain	110 – 297	188	ATP-grasp
	Nucleotide binding	137 – 192	56	ATP By similarity
Experimental info
	Sequence conflict	477 – 478	2	MT → IA in AAC27548. Ref.1
	Sequence conflict	491	1	T → F in AAC27548. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O74197-1 [UniParc]. Last modified August 16, 2004. Version 2. Checksum: 481BA9042F0C532A	FASTA	570	62,712
	10 20 30 40 50 60 MDSRTVGILG GGQLGRMIVE AANRLNIKTL ILDAPNSPAK QITNASDHVD GSFANKEDIE 70 80 90 100 110 120 ALAAKCDVMT VEIEHVDVNA LKEVQKQFPK LEIYPTPETI GLIQDKYVQK QHLVKNRIPV 130 140 150 160 170 180 VPSITVENSK ESLIKTGSSL GYPFVLKSRT LAYDGRGNFV VKSEEDIEKG LEFLANRPLY 190 200 210 220 230 240 AEKWASFKKE LSVMIIRSLD GRVYSYPIVE TIHKNNVCHL CYVPARVPDS VQHKAKLMAE 250 260 270 280 290 300 NAIKSFPGCG IFGVEMFLLD DDSIVLNEIA PRPHNSGHYT INACVVSQFE AHLRAILDLP 310 320 330 340 350 360 MPKNFTSLST NSTNAIMLNL LGDPETKDKE LQICERALNT PGASVYLYGK ESKPNRKVGH 370 380 390 400 410 420 INIVCSSMKE CDKRLQFIMG LKTDPIKHSV MEILNAEEVR KPLVGVIMGS DSDLPVMSAA 430 440 450 460 470 480 CKMLEQFEVP FEVTIVSAHR TPYRMNKYAS EAVSRGIKVI IAGAGGAAHL PGMVAAMTPV 490 500 510 520 530 540 PVIGVPVKGS TLDGVDSLHS IVQMPRGVPV ATVAINNSTN AGILAVRMLG IHNYDYVKKM 550 560 570 EEFLNKQEEE VLQKAAKLES IGYEKYLESK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A high-copy-number ADE2-bearing plasmid for transformation of Candida glabrata." Hanic-Joyce P.J., Joyce P.B.M. Gene 211:395-400(1998) [PubMed: 9602176] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 2001 / CBS 138 / IFO 0622 / NRRL Y-65.
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 2001 / CBS 138 / IFO 0622 / NRRL Y-65.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF030388 Genomic DNA. Translation: AAC27548.1. CR380957 Genomic DNA. Translation: CAG61632.1.
RefSeq	XP_448669.1.
3D structure databases
HSSP	HSSP built from PDB template 1O4V based on UniProtKB Q9WYS7.
ModBase	Search...
Protein-protein interaction databases
STRING	O74197.
Genome annotation databases
GeneID	2890129.
GenomeReviews	Gene locus CAGL0K10340g in contig CR380957_GR.
KEGG	cgr:CAGL0K10340g.
Phylogenomic databases
HOGENOM	O74197.
OMA	LYAEKWA.
Enzyme and pathway databases
BRENDA	4.1.1.21. 189220.
Family and domain databases
InterPro	IPR016301. AIR_COase. IPR005875. AIR_COase_ATPase-su. IPR000031. AIR_COase_core. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.40.50.7700. AIR_carboxyl. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PANTHER	PTHR23047:SF1. PurK_ATP. 1 hit.
Pfam	PF00731. AIRC. 1 hit. PF02222. ATP-grasp. 1 hit. [Graphical view]
PIRSF	PIRSF001340. AIR_carboxylase. 1 hit.
ProDom	PD002193. AIR_carboxyl. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01162. purE. 1 hit. TIGR01161. purK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR6_CANGA

Accession

Primary (citable) accession number: O74197
Secondary accession number(s): Q6FM75

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 16, 2004
Last modified:	November 3, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)