Reviewed, UniProtKB/Swiss-Prot O74196 (UBC1_COLGL)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2-16 kDa
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase
    Ubiquitin carrier protein
    Colletotrichum hard-surface-induced protein 1

Gene names

Name:	UBC1
Synonyms:	CHIP1

Organism

Colletotrichum gloeosporioides (Anthracnose fungus) (Glomerella cingulata)

Taxonomic identifier

474922 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreomycetidae incertae sedis › Glomerellaceae › Glomerella

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. May also mediate selective proteolysis pathways.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Developmental stage	Expressed in the conidium during the process of appressorium formation.
Induction	By hard-surface contact. Ethylene and the host surface wax enhance this induction which is essential for appressorium formation.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	Ubiquitin-conjugating enzyme E2-16 kDa		PRO_0000082542
Sites
	Active site	107	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O74196-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 6B65B0BE2F37D528	FASTA	147	16,528
	10 20 30 40 50 60 MAFKRINKEL TDLGRDPPSS CSAGPVGEDL FHWQATIMGP GDSPYSGGVF FLAIHFPTDY 70 80 90 100 110 120 PFKPPKVNFT TRIYHPNINS NGSICLDILR DQWSPALTIS KVLLSICSML TDPNPDEPLV 130 140 PEIAHVYKTD RARYEATARE WTRKYAI

References

[1]

"Identification of a gene product induced by hard-surface contact of Colletotrichum gloeosporioides conidia as a ubiquitin-conjugating enzyme by yeast complementation." Liu Z.-M., Kolattukudy P.E. J. Bacteriol. 180:3592-3597(1998) [PubMed: 9658002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Conidium.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF030296 mRNA. Translation: AAC39499.1.
3D structure databases
HSSP	HSSP built from PDB template 1QCQ based on UniProtKB P15731.
SMR	O74196. Positions 1-147.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.3.2.19. 39832.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC1_COLGL

Accession

Primary (citable) accession number: O74196

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	November 1, 1998
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)