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O74196

- UBC1_COLGL

UniProt

O74196 - UBC1_COLGL

Protein

Ubiquitin-conjugating enzyme E2-16 kDa

Gene

UBC1

Organism
Colletotrichum gloeosporioides (Anthracnose fungus) (Glomerella cingulata)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the covalent attachment of ubiquitin to other proteins. May also mediate selective proteolysis pathways.

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei107 – 1071Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein ubiquitination Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2-16 kDa (EC:6.3.2.19)
    Alternative name(s):
    Colletotrichum hard-surface-induced protein 1
    Ubiquitin carrier protein
    Ubiquitin-protein ligase
    Gene namesi
    Name:UBC1
    Synonyms:CHIP1
    OrganismiColletotrichum gloeosporioides (Anthracnose fungus) (Glomerella cingulata)
    Taxonomic identifieri474922 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeGlomerellalesGlomerellaceaemitosporic GlomerellaceaeColletotrichum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 147147Ubiquitin-conjugating enzyme E2-16 kDaPRO_0000082542Add
    BLAST

    Expressioni

    Developmental stagei

    Expressed in the conidium during the process of appressorium formation.

    Inductioni

    By hard-surface contact. Ethylene and the host surface wax enhance this induction which is essential for appressorium formation.

    Structurei

    3D structure databases

    ProteinModelPortaliO74196.
    SMRiO74196. Positions 1-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O74196-1 [UniParc]FASTAAdd to Basket

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    MAFKRINKEL TDLGRDPPSS CSAGPVGEDL FHWQATIMGP GDSPYSGGVF    50
    FLAIHFPTDY PFKPPKVNFT TRIYHPNINS NGSICLDILR DQWSPALTIS 100
    KVLLSICSML TDPNPDEPLV PEIAHVYKTD RARYEATARE WTRKYAI 147
    Length:147
    Mass (Da):16,528
    Last modified:November 1, 1998 - v1
    Checksum:i6B65B0BE2F37D528
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF030296 mRNA. Translation: AAC39499.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF030296 mRNA. Translation: AAC39499.1 .

    3D structure databases

    ProteinModelPortali O74196.
    SMRi O74196. Positions 1-147.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a gene product induced by hard-surface contact of Colletotrichum gloeosporioides conidia as a ubiquitin-conjugating enzyme by yeast complementation."
      Liu Z.-M., Kolattukudy P.E.
      J. Bacteriol. 180:3592-3597(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Conidium.

    Entry informationi

    Entry nameiUBC1_COLGL
    AccessioniPrimary (citable) accession number: O74196
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 17, 2003
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3