O74196 (UBC1_COLGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2-16 kDa
EC=6.3.2.19

Alternative name(s):
Colletotrichum hard-surface-induced protein 1
Ubiquitin carrier protein
Ubiquitin-protein ligase

Gene names

Name:	UBC1
Synonyms:	CHIP1

Organism

Colletotrichum gloeosporioides (Anthracnose fungus) (Glomerella cingulata)

Taxonomic identifier

474922 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Glomerellales › Glomerellaceae › Glomerella

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. May also mediate selective proteolysis pathways.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Developmental stage	Expressed in the conidium during the process of appressorium formation.
Induction	By hard-surface contact. Ethylene and the host surface wax enhance this induction which is essential for appressorium formation.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	Ubiquitin-conjugating enzyme E2-16 kDa		PRO_0000082542
Sites
	Active site	107	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O74196 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 6B65B0BE2F37D528	FASTA	147	16,528
	10 20 30 40 50 60 MAFKRINKEL TDLGRDPPSS CSAGPVGEDL FHWQATIMGP GDSPYSGGVF FLAIHFPTDY 70 80 90 100 110 120 PFKPPKVNFT TRIYHPNINS NGSICLDILR DQWSPALTIS KVLLSICSML TDPNPDEPLV 130 140 PEIAHVYKTD RARYEATARE WTRKYAI

References

[1]	"Identification of a gene product induced by hard-surface contact of Colletotrichum gloeosporioides conidia as a ubiquitin-conjugating enzyme by yeast complementation." Liu Z.-M., Kolattukudy P.E. J. Bacteriol. 180:3592-3597(1998) [PubMed: 9658002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Conidium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF030296 mRNA. Translation: AAC39499.1.
3D structure databases
ProteinModelPortal	O74196.
SMR	O74196. Positions 1-147.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC1_COLGL

Accession

Primary (citable) accession number: O74196

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	November 1, 1998
Last modified:	June 28, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program