O74192 (DAK_PICPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxyacetone kinase
Short name=DHA kinase
EC=2.7.1.29

Alternative name(s):
Glycerone kinase

Gene names

Name:

DAK

Organism

Pichia pastoris (Yeast)

Taxonomic identifier

4922 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Komagataella

Protein attributes

Sequence length	608 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycerone = ADP + glycerone phosphate.
Pathway	Polyol metabolism; glycerol fermentation; glycerone phosphate from glycerol (oxidative route): step 2/2.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the dihydroxyacetone kinase (DAK) family. Contains 1 DhaK domain. Contains 1 DhaL domain.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerone kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 608	608	Dihydroxyacetone kinase		PRO_0000121519
Regions
	Domain	8 – 357	350	DhaK
	Domain	392 – 599	208	DhaL
	Nucleotide binding	421 – 424	4	ATP By similarity
	Nucleotide binding	467 – 468	2	ATP By similarity
	Nucleotide binding	523 – 524	2	ATP By similarity
	Nucleotide binding	584 – 586	3	ATP By similarity
	Region	53 – 56	4	Substrate binding By similarity
Sites
	Active site	234	1	Tele-hemiaminal-histidine intermediate By similarity
	Binding site	105	1	Substrate By similarity
	Binding site	110	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O74192 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 412F00BA4D965119	FASTA	608	65,312
	10 20 30 40 50 60 MSSKHWDYKK DLVLSHLAGL CQSNPHVRLI ESERVVISAE NQEDKITLIS GGGSGHEPLH 70 80 90 100 110 120 AGFVTKDGLL DAAVAGFIFA SPSTKQIFSA IKAKPSKKGT LIIVKNYTGD ILHFGLAAEK 130 140 150 160 170 180 AKAEGLNAEL LIVQDDVSVG KAKNGLVGRR GLAGTSLVHK ILGAKAYLQK DNLELHQLVT 190 200 210 220 230 240 FGEKVVANLV TIGASLDHVT IPARANKQEE DDSDDEHGYE VLKHDEFEIG MGIHNEPGIK 250 260 270 280 290 300 KSSPIPTVDE LVAELLEYLL STTDKDRNYV QFDKNDEVVL LINNLGGTSV LELYAIQNIV 310 320 330 340 350 360 VDQLASKYSI KPVRIFTGTF TTSLDGPGFS ITLLNATKTG DKDILKFLDH KTSAPGWNSN 370 380 390 400 410 420 ISDWSGRVDN FIVAAPEIDE GDSSSKVSVD AKLYADLLES GVKKVISKEP KITLYDTVAG 430 440 450 460 470 480 DGDCGETLAN GSNAILKALA EGKLDLKDGV KSLVQITDIV ETAMGGTSGG LYSIFISALA 490 500 510 520 530 540 KSLKEKELSE GAYTLTLETI SGSLQAALQS LFKYTRARTG DRTLIDALEP FVKEFAKSKD 550 560 570 580 590 600 LKLANKAAHD GAEATRKLEA KFGRASYVAE EEFKQFESEG GLPDPGAIGL AALISGITDA YFKSETKL

References

[1]	"The Pichia pastoris dihydroxyacetone kinase is a PTS1-containing, but cytosolic, protein that is essential for growth on methanol." Luers G.H., Advani R., Wenzel T., Subramani S. Yeast 14:759-771(1998) [PubMed: 9675820] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF019198 Genomic DNA. Translation: AAC39490.1.
3D structure databases
ProteinModelPortal	O74192.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
PhylomeDB	O74192.
Enzyme and pathway databases
BRENDA	2.7.1.29. 4827.
Family and domain databases
InterPro	IPR004006. Dak1. IPR004007. Dak2. IPR012734. DhaK_ATP. [Graphical view]
Pfam	PF02733. Dak1. 1 hit. PF02734. Dak2. 1 hit. [Graphical view]
SUPFAM	SSF101473. Dak2. 1 hit.
TIGRFAMs	TIGR02361. Dak_ATP. 1 hit.
PROSITE	PS51481. DHAK. 1 hit. PS51480. DHAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAK_PICPA

Accession

Primary (citable) accession number: O74192

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program