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Protein

Target of rapamycin complex subunit wat1

Gene

pop3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

component of both TORC1 and TORC2, which regulate multiple cellular processes to control cell growth in response to environmental signals. Nutrient limitation and environmental stress signals cause inactivation of TORC1. Active TORC1 positively controls cell growth and ribosome biogenesis by regulating ribosomal protein gene expression. TORC1 negatively controls G1 cell-cycle arrest, sexual development and amino acid uptake. Represses mating, meiosis and sporulation efficiency by interfering with the functions of the transcription factor ste11 and the meiosis-promoting RNA-binding protein mei2. TORC2 is required for cell survival under various stress conditions. TORC2 positively controls G1 cell-cycle arrest, sexual development and amino acid uptake. Positively regulates amino acid uptake through the control of expression of amino acid permeases (PubMed:17046992, PubMed:18076573, PubMed:17261596). May play a role in mRNA maturation as a coupling protein between splicing and synthesis and/or stabilization.4 Publications

GO - Biological processi

  • meiotic cell cycle Source: UniProtKB-KW
  • microtubule cytoskeleton organization Source: PomBase
  • mitotic sister chromatid segregation Source: PomBase
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Meiosis, Sporulation

Enzyme and pathway databases

ReactomeiR-SPO-3371571. HSF1-dependent transactivation.

Names & Taxonomyi

Protein namesi
Recommended name:
Target of rapamycin complex subunit wat11 Publication
Short name:
TORC subunit wat1
Alternative name(s):
WD repeat-containing protein pop3
Gene namesi
Name:pop31 Publication
Synonyms:wat11 Publication
ORF Names:SPBC21B10.05cImported
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC21B10.05c.
PomBaseiSPBC21B10.05c. pop3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nucleus Source: PomBase
  • TORC1 complex Source: PomBase
  • TORC2 complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314Target of rapamycin complex subunit wat1PRO_0000051141Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei141 – 1411Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

The target of rapamycin complex 1 (TORC1) is composed of at least mip1, pop3/wat1, tco89, toc1 and tor2. The target of rapamycin complex 2 (TORC2) is composed of at least bit61, pop3/wat1, sin1, ste20 and tor1 (PubMed:17046992, PubMed:18076573, PubMed:17261596). Interacts with prp2 (PubMed:11686295).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
prp2P366294EBI-1564139,EBI-1024157

Protein-protein interaction databases

BioGridi277170. 14 interactions.
IntActiO74184. 4 interactions.
MINTiMINT-4678059.

Structurei

3D structure databases

ProteinModelPortaliO74184.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1 – 3535WD 1Add
BLAST
Repeati38 – 7639WD 2Add
BLAST
Repeati81 – 12040WD 3Add
BLAST
Repeati122 – 16140WD 4Add
BLAST
Repeati165 – 20440WD 5Add
BLAST
Repeati213 – 25240WD 6Add
BLAST
Repeati257 – 29640WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat LST8 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000208395.
InParanoidiO74184.
KOiK08266.
OMAiAGHHTVK.
OrthoDBiEOG7C8GSW.
PhylomeDBiO74184.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74184-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVQYPPQHS VLLVSSGYDH TIRFWEALSG ICSRTIQHAD SQVNRLCISP
60 70 80 90 100
DKKFLAAAGN PHVRLYDINT SSQMPLMTFE GHTNNVTAIA FHCDGKWLAT
110 120 130 140 150
SSEDGTVKVW DMRAPSVQRN YDHKSPVNDL LIHPNQGELL SCDQSGRVRA
160 170 180 190 200
WDLGENSCTH ELIPEEDVPM SSITVGSDGS MLIAGNNKGN CYVWRMLNHQ
210 220 230 240 250
GASLLQPVVK FQAHQRYITR CVLSPDVKHL ATCSADATVN IWSTEDMSFM
260 270 280 290 300
LERRLQGHQR WVWDCAFSAD STYLVTASSD HVARLWELSS GETIRQYSGH
310
HKAAVCVALN DYQI
Length:314
Mass (Da):35,133
Last modified:November 1, 1998 - v1
Checksum:iDC6D1275BDF1E086
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016895 Genomic DNA. Translation: BAA32427.1.
CU329671 Genomic DNA. Translation: CAB57925.1.
PIRiT39922.
RefSeqiNP_595682.1. NM_001021577.2.

Genome annotation databases

EnsemblFungiiSPBC21B10.05c.1; SPBC21B10.05c.1:pep; SPBC21B10.05c.
GeneIDi2540645.
KEGGispo:SPBC21B10.05c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016895 Genomic DNA. Translation: BAA32427.1.
CU329671 Genomic DNA. Translation: CAB57925.1.
PIRiT39922.
RefSeqiNP_595682.1. NM_001021577.2.

3D structure databases

ProteinModelPortaliO74184.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277170. 14 interactions.
IntActiO74184. 4 interactions.
MINTiMINT-4678059.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC21B10.05c.1; SPBC21B10.05c.1:pep; SPBC21B10.05c.
GeneIDi2540645.
KEGGispo:SPBC21B10.05c.

Organism-specific databases

EuPathDBiFungiDB:SPBC21B10.05c.
PomBaseiSPBC21B10.05c. pop3.

Phylogenomic databases

HOGENOMiHOG000208395.
InParanoidiO74184.
KOiK08266.
OMAiAGHHTVK.
OrthoDBiEOG7C8GSW.
PhylomeDBiO74184.

Enzyme and pathway databases

ReactomeiR-SPO-3371571. HSF1-dependent transactivation.

Miscellaneous databases

PROiO74184.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A wat1 mutant of fission yeast is defective in cell morphology."
    Kemp J.T., Balasubramanian M.K., Gould K.L.
    Mol. Gen. Genet. 254:127-138(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Conserved Wat1/Pop3 WD-repeat protein of fission yeast secures genome stability through microtubule integrity and may be involved in mRNA maturation."
    Ochotorena I.L., Hirata D., Kominami K., Potashkin J., Sahin F., Wentz-Hunter K., Gould K.L., Sato K., Yoshida Y., Vardy L., Toda T.
    J. Cell Sci. 114:2911-2920(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH PRP2.
    Strain: 972 / ATCC 24843.
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. "Fission yeast Tor2 promotes cell growth and represses cell differentiation."
    Alvarez B., Moreno S.
    J. Cell Sci. 119:4475-4485(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TOR1 AND TOR2.
  5. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. "Rapamycin sensitivity of the Schizosaccharomyces pombe tor2 mutant and organization of two highly phosphorylated TOR complexes by specific and common subunits."
    Hayashi T., Hatanaka M., Nagao K., Nakaseko Y., Kanoh J., Kokubu A., Ebe M., Yanagida M.
    Genes Cells 12:1357-1370(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TORC1 AND THE TORC2 COMPLEXES, PHOSPHORYLATION AT SER-141, IDENTIFICATION BY MASS SPECTROMETRY.
  7. "Loss of the TOR kinase Tor2 mimics nitrogen starvation and activates the sexual development pathway in fission yeast."
    Matsuo T., Otsubo Y., Urano J., Tamanoi F., Yamamoto M.
    Mol. Cell. Biol. 27:3154-3164(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TOR1 AND TOR2.

Entry informationi

Entry nameiWAT1_SCHPO
AccessioniPrimary (citable) accession number: O74184
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: November 1, 1998
Last modified: June 8, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.