O74168 (MANBA_ASPAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable beta-mannosidase A
EC=3.2.1.25

Alternative name(s):
Mannanase A
Short name=Mannase A

Gene names

Name:	mndA
Synonyms:	manB

Organism

Aspergillus aculeatus

Taxonomic identifier

5053 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	937 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides. Involved in the degradation of mannan and galactomannan By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.
Pathway	Glycan metabolism; N-glycan degradation.
Subunit structure	Homodimer By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 937	914	Probable beta-mannosidase A		PRO_0000394642
Sites
	Active site	482	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	42	1	N-linked (GlcNAc...) Potential
	Glycosylation	82	1	N-linked (GlcNAc...) Potential
	Glycosylation	250	1	N-linked (GlcNAc...) Potential
	Glycosylation	285	1	N-linked (GlcNAc...) Potential
	Glycosylation	319	1	N-linked (GlcNAc...) Potential
	Glycosylation	329	1	N-linked (GlcNAc...) Potential
	Glycosylation	350	1	N-linked (GlcNAc...) Potential
	Glycosylation	553	1	N-linked (GlcNAc...) Potential
	Glycosylation	612	1	N-linked (GlcNAc...) Potential
	Glycosylation	743	1	N-linked (GlcNAc...) Potential
	Glycosylation	796	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O74168 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 0F6200BEB03D704B	FASTA	937	104,216
	10 20 30 40 50 60 MRALPTTATT LLGVLFFPSA SRSQYVRDLG TEQWTLSSAT LNRTVPAQFP SQVHMDLLRE 70 80 90 100 110 120 GIIDEPYNDL NDFNLRWIAD ANWTYTSGKI EGLGEDYEST WLVFDGLDTF ASISFCGQFV 130 140 150 160 170 180 GATDNQFRQY MFDVSSILKA CPEEPTLGIQ FGSAPNIVDA IAQDPSSPTW PEGVQITYEY 190 200 210 220 230 240 PNRWFMRKEQ SDFGWDWGPA FAPAGPWKPG YVVQLKQAAP VYVRNTDLDI YRLGQINYLP 250 260 270 280 290 300 PDQTQPWVVN ASLDYLGSLP ENPSMAIEVK DLQSGEILAS RPLTNITVTE GSVTGVTVLE 310 320 330 340 350 360 GVDPKLWWPQ GLGDQNLYNV TISVTDGGNQ SVAEVTKRTG FRTIFLNQRN ITDAQLAQGI 370 380 390 400 410 420 APGANWHFEV NGHEFYAKGS NLIPPDCFWT RVTEDTMTRL FDAVVAGNQN MLRVWSSGAY 430 440 450 460 470 480 LHDYIYDLAD EKGILLCSEF QFSDALYPTD DAFLENVAAE VVYNVRRVNH HPSLALWAGG 490 500 510 520 530 540 NEIESLMLLL VEAADPESYP FYVGEYEKMY ISLFLPLVYE NTRSISYSPS STTEGYLDID 550 560 570 580 590 600 LSAPVPMAER YSNTTEGEYY GDTDHYNYDA SIAFDYGTYP VGRFANEFGF HSMPSLQTWQ 610 620 630 640 650 660 QALTDPADLT FNSSVVMLRN HHYPAGGLMT DNYHNTVARH GRNDPGRAGL LPDAQHSVRP 670 680 690 700 710 720 RGQLQRLVPR DPALPGGPLQ VTNPVLPAGQ RAARTPARVP VLAARGHLAG ALVGGDRVRR 730 740 750 760 770 780 PLEGPHYVAR DIYKPVIVSP FWNYTTGALD IYVTSDLWTA AAGSVTLTWR DLSGKPIASN 790 800 810 820 830 840 GGLPTKPLPF HVGALNSTRL YRMNMKQQPL PRHEDAILAL ELTATGSLPN TDEEVTFTHE 850 860 870 880 890 900 QWFTPAFPKD LDLVNLRVRV EYDAPLGKFA VEATAGVALY TWLEHPEGVV GYFEENSFVV 910 920 930 VPGQKKVVGF VVQADETDGE WVHDVTVRSL WDLNEGE

References

[1]

"Development of an efficient production method for beta-mannosidase by the creation of an overexpression system in Aspergillus aculeatus." Kanamasa S., Kawaguchi T., Takada G., Kajiwara S., Sumitani J., Arai M. Lett. Appl. Microbiol. 45:142-147(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], GLYCOSYLATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB015509 Genomic DNA. Translation: BAA29029.1.
3D structure databases
ProteinModelPortal	O74168.
ModBase	Search...
Protein family/group databases
CAZy	GH2. Glycoside Hydrolase Family 2.
mycoCLAP	MND2B_ASPAC.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00280.
Family and domain databases
Gene3D	2.60.40.320. 1 hit. 3.20.20.80. 1 hit.
InterPro	IPR008979. Galactose-bd-like. IPR013812. Glyco_hydro_2/20_Ig-like. IPR006102. Glyco_hydro_2_Ig-like. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00703. Glyco_hydro_2. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF49303. Glyco_hydro_2Ig. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. False negative. PS00608. GLYCOSYL_HYDROL_F2_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MANBA_ASPAC

Accession

Primary (citable) accession number: O74168

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program