Reviewed, UniProtKB/Swiss-Prot O74165 (FDFT_PICJA)

Names and origin

Protein names

Recommended name:
    Squalene synthetase
      Short name=SQS
      Short name=SS
    EC=2.5.1.21
Alternative name(s):
    Farnesyl-diphosphate farnesyltransferase
    FPP:FPP farnesyltransferase

Gene names

Name:

ERG9

Organism

Pichia jadinii (Yeast) (Candida utilis)

Taxonomic identifier

4903 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 farnesyl diphosphate = diphosphate + presqualene diphosphate. Presqualene diphosphate + NAD(P)H = squalene + diphosphate + NAD(P)+.
Cofactor	Magnesium.
Pathway	Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the phytoene/squalene synthetase family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Isoprene biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	Magnesium NADP
Molecular function	Oxidoreductase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	farnesyl-diphosphate farnesyltransferase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Squalene synthetase		PRO_0000067450
Regions
	Transmembrane	291 – 311	21	Potential
	Transmembrane	423 – 443	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	O74165-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: E7231500BBF5D82B	FASTA	443	50,954
	10 20 30 40 50 60 MGKLLQLALH PDELASIVQF KLFRKNENAR NPATESAELI RCYELLNLTS RSFAAVIEEL 70 80 90 100 110 120 HPELRNVIMV FYLVLRALDT VEVDMSIENS VKLPVLRQFH EKLDTKDWTF DGNSPNEKDR 130 140 150 160 170 180 CVLVEFDRIL GQYHELKPQY QKVIKEITEK MGNGMADYIE NENFNSNGLL TIEDYDLYCY 190 200 210 220 230 240 YVAGLVGDGL TQLIVLAKFG NSELSVNKQL FKSMGLFLQK TNIIRDYEED QVDGRAFWPK 250 260 270 280 290 300 EIWGKYANEL SDFMKPENQS QGLWCISELV CNALDHVIDV LQYLALVEEQ TSFNFCAIPQ 310 320 330 340 350 360 VMAIATLELV FQNPQVLTQH VKIRKGTTVS LILESRTLEG CARIFRRYLR KIHHKSHPSD 370 380 390 400 410 420 PNYLRLGITI GKIEQFLDGM YPHYVPKGIT PQTTSIRTQV VKRLQLDEPM KRDIDEEILK 430 440 TRILLLSLGV AVFGVVYGVV RII

References

[1]

"Increased carotenoid production by the food yeast Candida utilis through metabolic engineering of the isoprenoid pathway." Shimada H., Kondo K., Fraser P.D., Miura Y., Saito T., Misawa N. Appl. Environ. Microbiol. 64:2676-2680(1998) [PubMed: 9647847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB012604 Genomic DNA. Translation: BAA31938.1.
3D structure databases
SMR	O74165. Positions 39-374.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.21. 288941.
Family and domain databases
InterPro	IPR002060. Squ/phyt_synthse. IPR006449. Squal_synth. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
TIGRFAMs	TIGR01559. squal_synth. 1 hit.
PROSITE	PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDFT_PICJA

Accession

Primary (citable) accession number: O74165

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	November 1, 1998
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)