O74165 (FDFT_CYBJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Squalene synthase
Short name=SQS
Short name=SS
EC=2.5.1.21

Alternative name(s):
FPP:FPP farnesyltransferase
Farnesyl-diphosphate farnesyltransferase

Gene names

Name:

ERG9

Organism

Cyberlindnera jadinii (Torula yeast) (Pichia jadinii)

Taxonomic identifier

4903 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Phaffomycetaceae › Cyberlindnera

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of 2 two farnesyl pyrophosphate moieties to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. Required for the biosynthesis of ergosterol. Ref.1
Catalytic activity	2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.
Cofactor	Magnesium By similarity.
Pathway	Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Lipid biosynthesis Lipid metabolism Steroid biosynthesis Steroid metabolism Sterol biosynthesis Sterol metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium NADP
Molecular function	Oxidoreductase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological_process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	farnesyl-diphosphate farnesyltransferase activity Inferred from electronic annotation. Source: EC oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW squalene synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Squalene synthase		PRO_0000067450
Regions
	Transmembrane	291 – 311	21	Helical; Potential
	Transmembrane	423 – 443	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	O74165 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: E7231500BBF5D82B	FASTA	443	50,954
	10 20 30 40 50 60 MGKLLQLALH PDELASIVQF KLFRKNENAR NPATESAELI RCYELLNLTS RSFAAVIEEL 70 80 90 100 110 120 HPELRNVIMV FYLVLRALDT VEVDMSIENS VKLPVLRQFH EKLDTKDWTF DGNSPNEKDR 130 140 150 160 170 180 CVLVEFDRIL GQYHELKPQY QKVIKEITEK MGNGMADYIE NENFNSNGLL TIEDYDLYCY 190 200 210 220 230 240 YVAGLVGDGL TQLIVLAKFG NSELSVNKQL FKSMGLFLQK TNIIRDYEED QVDGRAFWPK 250 260 270 280 290 300 EIWGKYANEL SDFMKPENQS QGLWCISELV CNALDHVIDV LQYLALVEEQ TSFNFCAIPQ 310 320 330 340 350 360 VMAIATLELV FQNPQVLTQH VKIRKGTTVS LILESRTLEG CARIFRRYLR KIHHKSHPSD 370 380 390 400 410 420 PNYLRLGITI GKIEQFLDGM YPHYVPKGIT PQTTSIRTQV VKRLQLDEPM KRDIDEEILK 430 440 TRILLLSLGV AVFGVVYGVV RII

References

[1]

"Increased carotenoid production by the food yeast Candida utilis through metabolic engineering of the isoprenoid pathway." Shimada H., Kondo K., Fraser P.D., Miura Y., Saito T., Misawa N. Appl. Environ. Microbiol. 64:2676-2680(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB012604 Genomic DNA. Translation: BAA31938.1.
3D structure databases
ProteinModelPortal	O74165.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00767; UER00751.
Family and domain databases
Gene3D	1.10.600.10. 1 hit.
InterPro	IPR002060. Squ/phyt_synthse. IPR006449. Squal_synth. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
TIGRFAMs	TIGR01559. squal_synth. 1 hit.
PROSITE	PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDFT_CYBJA

Accession

Primary (citable) accession number: O74165

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	November 1, 1998
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program