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O74165 (FDFT_CYBJA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Squalene synthase

Short name=SQS
Short name=SS
EC=2.5.1.21
Alternative name(s):
FPP:FPP farnesyltransferase
Farnesyl-diphosphate farnesyltransferase
Gene names
Name:ERG9
OrganismCyberlindnera jadinii (Torula yeast) (Pichia jadinii)
Taxonomic identifier4903 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesPhaffomycetaceaeCyberlindnera

Protein attributes

Sequence length443 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the condensation of 2 two farnesyl pyrophosphate moieties to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. Required for the biosynthesis of ergosterol. Ref.1

Catalytic activity

2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.

Cofactor

Magnesium By similarity.

Pathway

Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the phytoene/squalene synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 443443Squalene synthase
PRO_0000067450

Regions

Transmembrane291 – 31121Helical; Potential
Transmembrane423 – 44321Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
O74165 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: E7231500BBF5D82B

FASTA44350,954
        10         20         30         40         50         60 
MGKLLQLALH PDELASIVQF KLFRKNENAR NPATESAELI RCYELLNLTS RSFAAVIEEL 

        70         80         90        100        110        120 
HPELRNVIMV FYLVLRALDT VEVDMSIENS VKLPVLRQFH EKLDTKDWTF DGNSPNEKDR 

       130        140        150        160        170        180 
CVLVEFDRIL GQYHELKPQY QKVIKEITEK MGNGMADYIE NENFNSNGLL TIEDYDLYCY 

       190        200        210        220        230        240 
YVAGLVGDGL TQLIVLAKFG NSELSVNKQL FKSMGLFLQK TNIIRDYEED QVDGRAFWPK 

       250        260        270        280        290        300 
EIWGKYANEL SDFMKPENQS QGLWCISELV CNALDHVIDV LQYLALVEEQ TSFNFCAIPQ 

       310        320        330        340        350        360 
VMAIATLELV FQNPQVLTQH VKIRKGTTVS LILESRTLEG CARIFRRYLR KIHHKSHPSD 

       370        380        390        400        410        420 
PNYLRLGITI GKIEQFLDGM YPHYVPKGIT PQTTSIRTQV VKRLQLDEPM KRDIDEEILK 

       430        440 
TRILLLSLGV AVFGVVYGVV RII 

« Hide

References

[1]"Increased carotenoid production by the food yeast Candida utilis through metabolic engineering of the isoprenoid pathway."
Shimada H., Kondo K., Fraser P.D., Miura Y., Saito T., Misawa N.
Appl. Environ. Microbiol. 64:2676-2680(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB012604 Genomic DNA. Translation: BAA31938.1.

3D structure databases

ProteinModelPortalO74165.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00767; UER00751.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
InterProIPR002060. Squ/phyt_synthse.
IPR006449. Squal_synth.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMSSF48576. SSF48576. 1 hit.
TIGRFAMsTIGR01559. squal_synth. 1 hit.
PROSITEPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFDFT_CYBJA
AccessionPrimary (citable) accession number: O74165
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: November 1, 1998
Last modified: February 19, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways