Reviewed,
UniProtKB/Swiss-Prot O74164 (HMDH_PICJA)
Last modified
November 25, 2008.
Version 55.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 3-hydroxy-3-methylglutaryl-coenzyme A reductase Short name=HMG-CoA reductase EC=1.1.1.34 | ||
| Gene names |
| ||
| Organism | Pichia jadinii (Yeast) (Candida utilis) | ||
| Taxonomic identifier | 4903 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia |
Protein attributes
| Sequence length | 934 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of the sterol biosynthesis. |
| Catalytic activity | (R)-mevalonate + CoA + 2 NADP(+) = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH. |
| Pathway | |
| Subcellular location | Endoplasmic reticulum membrane; Multi-pass membrane protein. |
| Sequence similarities | Belongs to the HMG-CoA reductase family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis |
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Transmembrane |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| PTM | Glycoprotein |
Gene Ontology (GO) | |
| Biological process | cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW coenzyme A metabolic processInferred from electronic annotation. Source: InterPro isoprenoid biosynthetic processInferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: InterPro |
| Molecular function | NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 934 | 934 | 3-hydroxy-3-methylglutaryl-coenzyme A reductase | PRO_0000114454 | |||||
Regions | |||||||||
| Transmembrane | 112 – 132 | 21 | Potential | ||||||
| Transmembrane | 142 – 162 | 21 | Potential | ||||||
| Transmembrane | 257 – 277 | 21 | Potential | ||||||
| Transmembrane | 335 – 355 | 21 | Potential | ||||||
| Transmembrane | 422 – 442 | 21 | Potential | ||||||
| Region | 443 – 521 | 79 | Linker | ||||||
| Region | 522 – 934 | 413 | Catalytic | ||||||
Sites | |||||||||
| Active site | 618 | 1 | Charge relay system By similarity | ||||||
| Active site | 752 | 1 | Charge relay system By similarity | ||||||
| Active site | 828 | 1 | Charge relay system By similarity | ||||||
| Active site | 924 | 1 | Proton donor By similarity | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 361 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 364 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 382 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 682 | 1 | N-linked (GlcNAc...) Potential | ||||||
Sequences
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References
| [1] | "Increased carotenoid production by the food yeast Candida utilis through metabolic engineering of the isoprenoid pathway." Shimada H., Kondo K., Fraser P.D., Miura Y., Saito T., Misawa N. Appl. Environ. Microbiol. 64:2676-2680(1998) [PubMed: 9647847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
Cross-references
Sequence databases | |
|---|---|
| AB012603 mRNA. Translation: BAA31937.1. | |
3D structure databases | |
| HSSP | HSSP built from PDB template 1DQA based on UniProtKB P04035. |
| ModBase | Search... |
Family and domain databases | |
| InterPro | IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. IPR000731. SSD_5TM. [Graphical view] |
| Gene3D | G3DSA:3.90.770.10. HMG-CoA_red. 1 hit. |
| PANTHER | PTHR10572. HMG-CoA_red. 1 hit. |
| Pfam | PF00368. HMG-CoA_red. 1 hit. [Graphical view] |
| PRINTS | PR00071. HMGCOARDTASE. |
| TIGRFAMs | TIGR00533. HMG_CoA_R_NADP. 1 hit. |
| PROSITE | PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. PS50156. SSD. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | HMDH_PICJA | ||||||||
| Accession | Primary (citable) accession number: O74164 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


