Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Orotidine 5'-phosphate decarboxylase

Gene

pyrF

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).UniRule annotation1 Publication

Catalytic activityi

Orotidine 5'-phosphate = UMP + CO2.UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11SubstrateUniRule annotation1
Binding sitei33SubstrateUniRule annotation1
Active sitei61Proton donorUniRule annotation1
Binding sitei114SubstrateUniRule annotation1
Binding sitei187Substrate; via amide nitrogenUniRule annotation1
Binding sitei188SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processPyrimidine biosynthesis

Enzyme and pathway databases

BioCyciTACI273075:G1GT7-78-MONOMER
UniPathwayiUPA00070; UER00120

Names & Taxonomyi

Protein namesi
Recommended name:
Orotidine 5'-phosphate decarboxylaseUniRule annotation (EC:4.1.1.23UniRule annotation)
Alternative name(s):
OMP decarboxylaseUniRule annotation
Short name:
OMPDCaseUniRule annotation
Short name:
OMPdecaseUniRule annotation
Gene namesi
Name:pyrFUniRule annotation
Ordered Locus Names:Ta0073
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
Proteomesi
  • UP000001024 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001346201 – 214Orotidine 5'-phosphate decarboxylaseAdd BLAST214

Proteomic databases

PRIDEiO74110

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi273075.Ta0073

Structurei

3D structure databases

ProteinModelPortaliO74110
SMRiO74110
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni59 – 68Substrate bindingUniRule annotation10
Regioni164 – 174Substrate bindingUniRule annotationAdd BLAST11

Sequence similaritiesi

Belongs to the OMP decarboxylase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00081 Archaea
COG0284 LUCA
HOGENOMiHOG000226069
KOiK01591
OMAiEMSHPGA
OrthoDBiPOG093Z0B4G

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01200_A OMPdecase_type1_A, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR014732 OMPdecase
IPR001754 OMPdeCOase_dom
IPR011060 RibuloseP-bd_barrel
PfamiView protein in Pfam
PF00215 OMPdecase, 1 hit
SMARTiView protein in SMART
SM00934 OMPdecase, 1 hit
SUPFAMiSSF51366 SSF51366, 1 hit
TIGRFAMsiTIGR01740 pyrF, 1 hit
PROSITEiView protein in PROSITE
PS00156 OMPDECASE, 1 hit

Sequencei

Sequence statusi: Complete.

O74110-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSESRLVVAL DLTDKDRALE IARSIGRQVF AIKINWPLVL AGSGSIIGEI
60 70 80 90 100
ARVSRVVCDF KIADIPNTNS IIAKFARDQG AWGIISHSFT GLESLRSVVE
110 120 130 140 150
ASGDTKVFSV VAMSHPGSDL INDNYRKLMD ISERAGVYGY IAPGNKLSDL
160 170 180 190 200
SEIRKRTKKT IMSPGIGSQG GRASDAIKAG ADLVIVGRSI YESADPVTAA
210
EAINEEIAKA VEQN
Length:214
Mass (Da):22,890
Last modified:December 1, 2000 - v2
Checksum:i1171F2C6AC058489
GO

Sequence cautioni

The sequence CAC11221 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti206E → G in AAC24044 (PubMed:10973825).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038845 Genomic DNA Translation: AAC24044.1
AL445063 Genomic DNA Translation: CAC11221.1 Different initiation.
PIRiT37332
RefSeqiWP_048161418.1, NC_002578.1

Genome annotation databases

EnsemblBacteriaiCAC11221; CAC11221; CAC11221
GeneIDi1455733
KEGGitac:Ta0073

Similar proteinsi

Entry informationi

Entry nameiPYRF_THEAC
AccessioniPrimary (citable) accession number: O74110
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: December 1, 2000
Last modified: March 28, 2018
This is version 100 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health