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Protein

50S ribosomal protein L10

Gene

rpl10

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Forms the large subunit's ribosomal stalk, playing a central role in the interaction of the ribosome with elongation factors; the stalk complex of P.horikoshii binds to E.coli large subunits and confers on them the ability to interact with eukaryotic elongation factors. Each succesive L12 dimer bound along the P0 spine increases the GTPase activity of elongation factors and increases translation by reconsituted ribosomes, although the first site is the most stimulatory.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-2001-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L10UniRule annotation
Alternative name(s):
Acidic ribosomal protein P0 homologUniRule annotation
Anchor protein P0
Gene namesi
Name:rpl10UniRule annotation
Synonyms:rplP0UniRule annotation
Ordered Locus Names:PH1999
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi217 – 2248LQKAYMHA → QQKAYMHQ: Binds less L12, about 50% GTPase activity, about 35% GTPase activity; when associated with 243-Q--Q250 or 272-Q--Q-279. 1 Publication
Mutagenesisi243 – 2508IQKAFLNA → QQKAFLNQ: About 65% GTPase activity; when associated with 272-Q--Q-279. About 35% GTPase activity; when associated with 217-Q--Q-224. 1 Publication
Mutagenesisi272 – 2798AFRAMLLL → QFRAMLLQ: About 65% GTPase activity; when associated with 243-Q--Q-250. About 35% GTPase activity; when associated with 217-Q--Q-224. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 34234250S ribosomal protein L10PRO_0000154804Add
BLAST

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit, binds large rRNA. Forms the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.2 Publications

Protein-protein interaction databases

STRINGi70601.PH1999.

Structurei

Secondary structure

1
342
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni8 – 103Combined sources
Helixi11 – 199Combined sources
Beta strandi23 – 297Combined sources
Helixi35 – 4713Combined sources
Beta strandi50 – 545Combined sources
Beta strandi56 – 594Combined sources
Helixi62 – 687Combined sources
Beta strandi78 – 803Combined sources
Beta strandi87 – 948Combined sources
Helixi96 – 10510Combined sources
Beta strandi190 – 1967Combined sources
Beta strandi199 – 2013Combined sources
Helixi203 – 2064Combined sources
Helixi210 – 23122Combined sources
Turni236 – 2383Combined sources
Helixi239 – 25618Combined sources
Turni262 – 2643Combined sources
Helixi265 – 28016Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A1YX-ray2.13G1-284[»]
ProteinModelPortaliO74109.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO74109.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni212 – 342131Required for interaction with ribosomal protein L12 dimersAdd
BLAST

Sequence similaritiesi

Belongs to the ribosomal protein L10P family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04288. Archaea.
COG0244. LUCA.
HOGENOMiHOG000229909.
KOiK02864.
OMAiIGTNDNP.

Family and domain databases

Gene3Di1.10.10.380. 2 hits.
HAMAPiMF_00280. Ribosomal_L10_arch.
InterProiIPR022909. 50S_L10_arch.
IPR013522. Ribosomal_L10_N_arc.
IPR001790. Ribosomal_L10P.
[Graphical view]
PfamiPF00466. Ribosomal_L10. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74109-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHVAEWKKK EVEELAKLIK SYPVIALVDV SSMPAYPLSQ MRRLIRENGG
60 70 80 90 100
LLRVSRNTLI ELAIKKAAKE LGKPELEKLV EYIDRGAGIL VTNMNPFKLY
110 120 130 140 150
KFLQQNRQPA PAKPGAVVPK DVVVPAGPTP LAPGPIVGQM QALGIPARIE
160 170 180 190 200
KGKVTIQKDT TVLKAGEVIT PELANILNAL GIQPLEVGLD VLAVYEDGIV
210 220 230 240 250
YTPDVLAIDE QEYIDMLQKA YMHAFNLAVN IAYPTPETIE AIIQKAFLNA
260 270 280 290 300
KTVAIEAGYI TKETIQDIIG RAFRAMLLLA QQLPEDVLDE KTKELLSAQA
310 320 330 340
QVAVATQPSE EEKKEEEKTE EEEKEEEASE EEALAGLSAL FG
Length:342
Mass (Da):37,514
Last modified:November 1, 1998 - v1
Checksum:iA469330FC19E6195
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA31126.1.
PIRiG71216.
RefSeqiWP_010886060.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA31126; BAA31126; BAA31126.
GeneIDi1442842.
KEGGipho:PH1999.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA31126.1.
PIRiG71216.
RefSeqiWP_010886060.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A1YX-ray2.13G1-284[»]
ProteinModelPortaliO74109.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1999.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA31126; BAA31126; BAA31126.
GeneIDi1442842.
KEGGipho:PH1999.

Phylogenomic databases

eggNOGiarCOG04288. Archaea.
COG0244. LUCA.
HOGENOMiHOG000229909.
KOiK02864.
OMAiIGTNDNP.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-2001-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO74109.

Family and domain databases

Gene3Di1.10.10.380. 2 hits.
HAMAPiMF_00280. Ribosomal_L10_arch.
InterProiIPR022909. 50S_L10_arch.
IPR013522. Ribosomal_L10_N_arc.
IPR001790. Ribosomal_L10P.
[Graphical view]
PfamiPF00466. Ribosomal_L10. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  2. "Three binding sites for stalk protein dimers are generally present in ribosomes from archaeal organism."
    Maki Y., Hashimoto T., Zhou M., Naganuma T., Ohta J., Nomura T., Robinson C.V., Uchiumi T.
    J. Biol. Chem. 282:32827-32833(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, RNA-BINDING, SUBUNIT.
  3. "Structural basis for translation factor recruitment to the eukaryotic/archaeal ribosomes."
    Naganuma T., Nomura N., Yao M., Mochizuki M., Uchiumi T., Tanaka I.
    J. Biol. Chem. 285:4747-4756(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.13 ANGSTROMS) OF 1-284, SUBUNIT, MUTAGENESIS OF 217-LEU--ALA-224; 243-ILE--ALA-250 AND 272-ALA--LEU-279.

Entry informationi

Entry nameiRL10_PYRHO
AccessioniPrimary (citable) accession number: O74109
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: December 9, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.