O74036 (RADA_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA repair and recombination protein RadA

Gene names

Name:	radA
Ordered Locus Names:	PF1926

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus furiosus

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules. HAMAP-Rule MF_00348
Sequence similarities	Belongs to the eukaryotic RecA-like protein family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA-dependent ATPase activity Inferred from electronic annotation. Source: HAMAP damaged DNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 349

349

DNA repair and recombination protein RadA HAMAP-Rule MF_00348

PRO_0000150104

Regions

Nucleotide binding

138 – 145

8

ATP Potential

Secondary structure

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349

Helix Strand Turn

Details...

Helix

45 – 52

8

Turn

53 – 55

3

Helix

59 – 63

5

Helix

67 – 74

8

Helix

78 – 91

14

Helix

100 – 108

9

Helix

118 – 123

6

Beta strand

126 – 129

4

Beta strand

132 – 137

6

Helix

144 – 154

11

Helix

159 – 161

3

Beta strand

167 – 175

9

Helix

179 – 188

10

Helix

193 – 198

6

Beta strand

200 – 204

5

Helix

208 – 225

18

Beta strand

228 – 230

3

Beta strand

232 – 238

7

Helix

242 – 247

6

Helix

251 – 253

3

Helix

254 – 275

22

Beta strand

278 – 283

6

Beta strand

311 – 317

7

Helix

319 – 321

3

Beta strand

323 – 329

7

Beta strand

331 – 333

3

Beta strand

336 – 343

8

Beta strand

346 – 348

3

Sequences

	Sequence		Length	Mass (Da)
	O74036 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 726E9DF1D67AB039	FASTA	349	38,399
	10 20 30 40 50 60 MAGEEVKEID EFEELGFEPA TEETPKKKKK EKIIRSIEDL PGVGPATAEK LREAGYDTLE 70 80 90 100 110 120 AIAVASPIEL KEVAGISEGT ALKIIQAARK AANLGTFMRA DEYLKKRATI GRISTGSKSL 130 140 150 160 170 180 DKLLGGGIET QAITEVFGEF GSGKTQLAHT LAVMVQLPPE EGGLNGSVIW IDTENTFRPE 190 200 210 220 230 240 RIREIAQNRG LDPDEVLKHI YVARAFNSNH QMLLVQQAED KIKELLNTDR PVKLLIVDSL 250 260 270 280 290 300 TSHFRSEYIG RGALAERQQK LAKHLADLHR LANLYDIAVF VTNQVQARPD AFFGDPTRPI 310 320 330 340 GGHILAHSAT LRVYLRKGKG GKRIARLIDA PHLPEGEAVF SITEKGIED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA repair systems in archaea: mementos from the last universal common ancestor?" DiRuggiero J., Brown J.R., Bogert A.P., Robb F.T. J. Mol. Evol. 49:474-484(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[3]	"Both RadA and RadB are involved in homologous recombination in Pyrococcus furiosus." Komori K., Miyata T., DiRuggiero J., Holley-Shanks R., Hayashi I., Cann I.K.O., Mayanagi K., Shinagawa H., Ishino Y. J. Biol. Chem. 275:33782-33790(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF052597 Genomic DNA. Translation: AAC34998.1.
AE009950 Genomic DNA. Translation: AAL82050.1.

RefSeq

NP_579655.1. NC_003413.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PZN	X-ray	2.85	A/B/C/D/E/F/G	1-349	[»]
4A6P	X-ray	1.50	A	108-349	[»]
4A6X	X-ray	1.55	A/B	108-349	[»]
4A74	X-ray	1.48	A/B	108-349	[»]
4A7O	X-ray	1.88	A	108-349	[»]
4B2I	X-ray	1.30	A	108-349	[»]
4B2L	X-ray	1.50	A	108-349	[»]
4B32	X-ray	1.50	A	108-349	[»]
4B33	X-ray	1.50	A	108-349	[»]
4B34	X-ray	1.55	A	108-349	[»]
4B35	X-ray	1.40	A	108-349	[»]
4B3B	X-ray	1.19	A	108-349	[»]
4B3C	X-ray	1.90	A	108-349	[»]
4B3D	X-ray	1.59	A/C	108-349	[»]

ProteinModelPortal

O74036.

SMR

O74036. Positions 35-349.

ModBase

Search...

Protein-protein interaction databases

STRING

186497.PF1926.

Proteomic databases

PRIDE

O74036.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAL82050; AAL82050; PF1926.

GeneID

1469806.

KEGG

pfu:PF1926.

Phylogenomic databases

eggNOG

COG0468.

HOGENOM

HOG000227426.

KO

K04483.

OMA

RAYNSDM.

ProtClustDB

PRK04301.

Family and domain databases

HAMAP

MF_00348. RadA.

InterPro

IPR003593. AAA+_ATPase.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR011938. DNA_recomb/repair_RadA.
IPR016467. DNA_recomb/repair_RecA-like.
IPR020588. DNA_recomb_RecA/RadB_ATP-bd.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]

PANTHER

PTHR22942:SF11. PTHR22942:SF11. 1 hit.

Pfam

PF08423. Rad51. 1 hit.
[Graphical view]

PIRSF

PIRSF005856. Rad51. 1 hit.

SMART

SM00382. AAA. 1 hit.
SM00278. HhH1. 2 hits.
[Graphical view]

SUPFAM

SSF47794. Rad51_N. 1 hit.

TIGRFAMs

TIGR02236. recomb_radA. 1 hit.

PROSITE

PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O74036.

Entry information

Entry name

RADA_PYRFU

Accession

Primary (citable) accession number: O74036

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program