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O74036

- RADA_PYRFU

UniProt

O74036 - RADA_PYRFU

Protein

DNA repair and recombination protein RadA

Gene

radA

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi138 – 1458ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. damaged DNA binding Source: UniProtKB-HAMAP
    3. DNA-dependent ATPase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-HAMAP
    2. DNA repair Source: UniProtKB-HAMAP

    Keywords - Biological processi

    DNA damage, DNA recombination

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA repair and recombination protein RadA
    Gene namesi
    Name:radA
    Ordered Locus Names:PF1926
    OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
    Taxonomic identifieri186497 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000001013: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 349349DNA repair and recombination protein RadAPRO_0000150104Add
    BLAST

    Proteomic databases

    PRIDEiO74036.

    Interactioni

    Protein-protein interaction databases

    STRINGi186497.PF1926.

    Structurei

    Secondary structure

    1
    349
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi45 – 528
    Turni53 – 553
    Helixi59 – 635
    Helixi67 – 748
    Helixi78 – 9114
    Helixi100 – 1089
    Helixi118 – 1236
    Turni124 – 1263
    Beta strandi127 – 13913
    Helixi144 – 15411
    Helixi159 – 1613
    Beta strandi167 – 1759
    Helixi179 – 18810
    Helixi193 – 1986
    Beta strandi200 – 2045
    Helixi208 – 22518
    Beta strandi228 – 2303
    Beta strandi232 – 2387
    Helixi242 – 2476
    Helixi253 – 27523
    Beta strandi278 – 2847
    Beta strandi310 – 3178
    Helixi319 – 3213
    Beta strandi322 – 3298
    Beta strandi331 – 3333
    Beta strandi338 – 3436
    Beta strandi346 – 3483

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1PZNX-ray2.85A/B/C/D/E/F/G1-349[»]
    4A6PX-ray1.50A108-349[»]
    4A6XX-ray1.55A/B108-349[»]
    4A74X-ray1.48A/B108-349[»]
    4A7OX-ray1.88A108-349[»]
    4B2IX-ray1.30A108-349[»]
    4B2LX-ray1.50A108-349[»]
    4B2PX-ray1.60A108-349[»]
    4B32X-ray1.50A108-349[»]
    4B33X-ray1.50A108-349[»]
    4B34X-ray1.55A108-349[»]
    4B35X-ray1.40A108-349[»]
    4B3BX-ray1.19A108-349[»]
    4B3CX-ray1.90A108-349[»]
    4B3DX-ray1.59A/C108-349[»]
    ProteinModelPortaliO74036.
    SMRiO74036. Positions 35-349.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO74036.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0468.
    HOGENOMiHOG000227426.
    KOiK04483.
    OMAiRAYNSDM.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_00348. RadA.
    InterProiIPR003593. AAA+_ATPase.
    IPR013632. DNA_recomb/repair_Rad51_C.
    IPR011938. DNA_recomb/repair_RadA.
    IPR016467. DNA_recomb/repair_RecA-like.
    IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR027417. P-loop_NTPase.
    IPR020588. RecA_ATP-bd.
    IPR020587. RecA_monomer-monomer_interface.
    [Graphical view]
    PfamiPF08423. Rad51. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005856. Rad51. 1 hit.
    SMARTiSM00382. AAA. 1 hit.
    SM00278. HhH1. 2 hits.
    [Graphical view]
    SUPFAMiSSF47794. SSF47794. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR02236. recomb_radA. 1 hit.
    PROSITEiPS50162. RECA_2. 1 hit.
    PS50163. RECA_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O74036-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGEEVKEID EFEELGFEPA TEETPKKKKK EKIIRSIEDL PGVGPATAEK    50
    LREAGYDTLE AIAVASPIEL KEVAGISEGT ALKIIQAARK AANLGTFMRA 100
    DEYLKKRATI GRISTGSKSL DKLLGGGIET QAITEVFGEF GSGKTQLAHT 150
    LAVMVQLPPE EGGLNGSVIW IDTENTFRPE RIREIAQNRG LDPDEVLKHI 200
    YVARAFNSNH QMLLVQQAED KIKELLNTDR PVKLLIVDSL TSHFRSEYIG 250
    RGALAERQQK LAKHLADLHR LANLYDIAVF VTNQVQARPD AFFGDPTRPI 300
    GGHILAHSAT LRVYLRKGKG GKRIARLIDA PHLPEGEAVF SITEKGIED 349
    Length:349
    Mass (Da):38,399
    Last modified:November 1, 1998 - v1
    Checksum:i726E9DF1D67AB039
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF052597 Genomic DNA. Translation: AAC34998.1.
    AE009950 Genomic DNA. Translation: AAL82050.1.
    RefSeqiNP_579655.1. NC_003413.1.
    WP_011013066.1. NC_003413.1.

    Genome annotation databases

    EnsemblBacteriaiAAL82050; AAL82050; PF1926.
    GeneIDi1469806.
    KEGGipfu:PF1926.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF052597 Genomic DNA. Translation: AAC34998.1 .
    AE009950 Genomic DNA. Translation: AAL82050.1 .
    RefSeqi NP_579655.1. NC_003413.1.
    WP_011013066.1. NC_003413.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1PZN X-ray 2.85 A/B/C/D/E/F/G 1-349 [» ]
    4A6P X-ray 1.50 A 108-349 [» ]
    4A6X X-ray 1.55 A/B 108-349 [» ]
    4A74 X-ray 1.48 A/B 108-349 [» ]
    4A7O X-ray 1.88 A 108-349 [» ]
    4B2I X-ray 1.30 A 108-349 [» ]
    4B2L X-ray 1.50 A 108-349 [» ]
    4B2P X-ray 1.60 A 108-349 [» ]
    4B32 X-ray 1.50 A 108-349 [» ]
    4B33 X-ray 1.50 A 108-349 [» ]
    4B34 X-ray 1.55 A 108-349 [» ]
    4B35 X-ray 1.40 A 108-349 [» ]
    4B3B X-ray 1.19 A 108-349 [» ]
    4B3C X-ray 1.90 A 108-349 [» ]
    4B3D X-ray 1.59 A/C 108-349 [» ]
    ProteinModelPortali O74036.
    SMRi O74036. Positions 35-349.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 186497.PF1926.

    Proteomic databases

    PRIDEi O74036.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL82050 ; AAL82050 ; PF1926 .
    GeneIDi 1469806.
    KEGGi pfu:PF1926.

    Phylogenomic databases

    eggNOGi COG0468.
    HOGENOMi HOG000227426.
    KOi K04483.
    OMAi RAYNSDM.

    Miscellaneous databases

    EvolutionaryTracei O74036.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    HAMAPi MF_00348. RadA.
    InterProi IPR003593. AAA+_ATPase.
    IPR013632. DNA_recomb/repair_Rad51_C.
    IPR011938. DNA_recomb/repair_RadA.
    IPR016467. DNA_recomb/repair_RecA-like.
    IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR027417. P-loop_NTPase.
    IPR020588. RecA_ATP-bd.
    IPR020587. RecA_monomer-monomer_interface.
    [Graphical view ]
    Pfami PF08423. Rad51. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005856. Rad51. 1 hit.
    SMARTi SM00382. AAA. 1 hit.
    SM00278. HhH1. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47794. SSF47794. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR02236. recomb_radA. 1 hit.
    PROSITEi PS50162. RECA_2. 1 hit.
    PS50163. RECA_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA repair systems in archaea: mementos from the last universal common ancestor?"
      DiRuggiero J., Brown J.R., Bogert A.P., Robb F.T.
      J. Mol. Evol. 49:474-484(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
    2. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
      Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
      Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
    3. "Both RadA and RadB are involved in homologous recombination in Pyrococcus furiosus."
      Komori K., Miyata T., DiRuggiero J., Holley-Shanks R., Hayashi I., Cann I.K.O., Mayanagi K., Shinagawa H., Ishino Y.
      J. Biol. Chem. 275:33782-33790(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiRADA_PYRFU
    AccessioniPrimary (citable) accession number: O74036
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3