Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA repair and recombination protein RadA

Gene

radA

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi138 – 1458ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. damaged DNA binding Source: UniProtKB-HAMAP
  3. DNA-dependent ATPase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.4.B7. 5243.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair and recombination protein RadA
Gene namesi
Name:radA
Ordered Locus Names:PF1926
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000001013 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349DNA repair and recombination protein RadAPRO_0000150104Add
BLAST

Proteomic databases

PRIDEiO74036.

Interactioni

Protein-protein interaction databases

STRINGi186497.PF1926.

Structurei

Secondary structure

1
349
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi45 – 528Combined sources
Turni53 – 553Combined sources
Helixi59 – 635Combined sources
Helixi67 – 748Combined sources
Helixi78 – 9114Combined sources
Helixi100 – 1089Combined sources
Helixi118 – 1236Combined sources
Turni124 – 1263Combined sources
Beta strandi127 – 13913Combined sources
Helixi144 – 15411Combined sources
Helixi159 – 1613Combined sources
Beta strandi167 – 1759Combined sources
Helixi179 – 18810Combined sources
Helixi193 – 1986Combined sources
Beta strandi200 – 2045Combined sources
Helixi208 – 22518Combined sources
Beta strandi228 – 2303Combined sources
Beta strandi232 – 2387Combined sources
Helixi242 – 2476Combined sources
Helixi253 – 27523Combined sources
Beta strandi278 – 2847Combined sources
Beta strandi310 – 3178Combined sources
Helixi319 – 3213Combined sources
Beta strandi322 – 3298Combined sources
Beta strandi331 – 3333Combined sources
Beta strandi338 – 3436Combined sources
Beta strandi346 – 3483Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PZNX-ray2.85A/B/C/D/E/F/G1-349[»]
4A6PX-ray1.48A/B108-349[»]
4A6XX-ray1.55A/B108-349[»]
4B2IX-ray1.30A108-349[»]
4B2LX-ray1.50A108-349[»]
4B2PX-ray1.60A108-349[»]
4B32X-ray1.50A108-349[»]
4B33X-ray1.50A108-349[»]
4B34X-ray1.55A108-349[»]
4B35X-ray1.40A108-349[»]
4B3BX-ray1.19A108-349[»]
4B3CX-ray1.90A108-349[»]
4B3DX-ray1.59A/C108-349[»]
4D6PX-ray1.48A/B108-349[»]
4UQOX-ray1.88A/B108-349[»]
ProteinModelPortaliO74036.
SMRiO74036. Positions 35-349.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO74036.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0468.
HOGENOMiHOG000227426.
KOiK04483.
OMAiRAYNSDM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00348. RadA.
InterProiIPR003593. AAA+_ATPase.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR011938. DNA_recomb/repair_RadA.
IPR016467. DNA_recomb/repair_RecA-like.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
PIRSFiPIRSF005856. Rad51. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00278. HhH1. 2 hits.
[Graphical view]
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02236. recomb_radA. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74036-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGEEVKEID EFEELGFEPA TEETPKKKKK EKIIRSIEDL PGVGPATAEK
60 70 80 90 100
LREAGYDTLE AIAVASPIEL KEVAGISEGT ALKIIQAARK AANLGTFMRA
110 120 130 140 150
DEYLKKRATI GRISTGSKSL DKLLGGGIET QAITEVFGEF GSGKTQLAHT
160 170 180 190 200
LAVMVQLPPE EGGLNGSVIW IDTENTFRPE RIREIAQNRG LDPDEVLKHI
210 220 230 240 250
YVARAFNSNH QMLLVQQAED KIKELLNTDR PVKLLIVDSL TSHFRSEYIG
260 270 280 290 300
RGALAERQQK LAKHLADLHR LANLYDIAVF VTNQVQARPD AFFGDPTRPI
310 320 330 340
GGHILAHSAT LRVYLRKGKG GKRIARLIDA PHLPEGEAVF SITEKGIED
Length:349
Mass (Da):38,399
Last modified:November 1, 1998 - v1
Checksum:i726E9DF1D67AB039
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF052597 Genomic DNA. Translation: AAC34998.1.
AE009950 Genomic DNA. Translation: AAL82050.1.
RefSeqiNP_579655.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL82050; AAL82050; PF1926.
GeneIDi1469806.
KEGGipfu:PF1926.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF052597 Genomic DNA. Translation: AAC34998.1.
AE009950 Genomic DNA. Translation: AAL82050.1.
RefSeqiNP_579655.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PZNX-ray2.85A/B/C/D/E/F/G1-349[»]
4A6PX-ray1.48A/B108-349[»]
4A6XX-ray1.55A/B108-349[»]
4B2IX-ray1.30A108-349[»]
4B2LX-ray1.50A108-349[»]
4B2PX-ray1.60A108-349[»]
4B32X-ray1.50A108-349[»]
4B33X-ray1.50A108-349[»]
4B34X-ray1.55A108-349[»]
4B35X-ray1.40A108-349[»]
4B3BX-ray1.19A108-349[»]
4B3CX-ray1.90A108-349[»]
4B3DX-ray1.59A/C108-349[»]
4D6PX-ray1.48A/B108-349[»]
4UQOX-ray1.88A/B108-349[»]
ProteinModelPortaliO74036.
SMRiO74036. Positions 35-349.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1926.

Chemistry

BindingDBiO74036.

Proteomic databases

PRIDEiO74036.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL82050; AAL82050; PF1926.
GeneIDi1469806.
KEGGipfu:PF1926.

Phylogenomic databases

eggNOGiCOG0468.
HOGENOMiHOG000227426.
KOiK04483.
OMAiRAYNSDM.

Enzyme and pathway databases

BRENDAi3.6.4.B7. 5243.

Miscellaneous databases

EvolutionaryTraceiO74036.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00348. RadA.
InterProiIPR003593. AAA+_ATPase.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR011938. DNA_recomb/repair_RadA.
IPR016467. DNA_recomb/repair_RecA-like.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
PIRSFiPIRSF005856. Rad51. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00278. HhH1. 2 hits.
[Graphical view]
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02236. recomb_radA. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DNA repair systems in archaea: mementos from the last universal common ancestor?"
    DiRuggiero J., Brown J.R., Bogert A.P., Robb F.T.
    J. Mol. Evol. 49:474-484(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  2. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  3. "Both RadA and RadB are involved in homologous recombination in Pyrococcus furiosus."
    Komori K., Miyata T., DiRuggiero J., Holley-Shanks R., Hayashi I., Cann I.K.O., Mayanagi K., Shinagawa H., Ishino Y.
    J. Biol. Chem. 275:33782-33790(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiRADA_PYRFU
AccessioniPrimary (citable) accession number: O74036
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1998
Last modified: April 1, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.