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O74036 (RADA_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA repair and recombination protein RadA
Gene names
Name:radA
Ordered Locus Names:PF1926
OrganismPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]
Taxonomic identifier186497 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules. HAMAP-Rule MF_00348

Sequence similarities

Belongs to the eukaryotic RecA-like protein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349DNA repair and recombination protein RadA HAMAP-Rule MF_00348
PRO_0000150104

Regions

Nucleotide binding138 – 1458ATP Potential

Secondary structure

................................................... 349
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O74036 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 726E9DF1D67AB039

FASTA34938,399
        10         20         30         40         50         60 
MAGEEVKEID EFEELGFEPA TEETPKKKKK EKIIRSIEDL PGVGPATAEK LREAGYDTLE 

        70         80         90        100        110        120 
AIAVASPIEL KEVAGISEGT ALKIIQAARK AANLGTFMRA DEYLKKRATI GRISTGSKSL 

       130        140        150        160        170        180 
DKLLGGGIET QAITEVFGEF GSGKTQLAHT LAVMVQLPPE EGGLNGSVIW IDTENTFRPE 

       190        200        210        220        230        240 
RIREIAQNRG LDPDEVLKHI YVARAFNSNH QMLLVQQAED KIKELLNTDR PVKLLIVDSL 

       250        260        270        280        290        300 
TSHFRSEYIG RGALAERQQK LAKHLADLHR LANLYDIAVF VTNQVQARPD AFFGDPTRPI 

       310        320        330        340 
GGHILAHSAT LRVYLRKGKG GKRIARLIDA PHLPEGEAVF SITEKGIED 

« Hide

References

« Hide 'large scale' references
[1]"DNA repair systems in archaea: mementos from the last universal common ancestor?"
DiRuggiero J., Brown J.R., Bogert A.P., Robb F.T.
J. Mol. Evol. 49:474-484(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[3]"Both RadA and RadB are involved in homologous recombination in Pyrococcus furiosus."
Komori K., Miyata T., DiRuggiero J., Holley-Shanks R., Hayashi I., Cann I.K.O., Mayanagi K., Shinagawa H., Ishino Y.
J. Biol. Chem. 275:33782-33790(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF052597 Genomic DNA. Translation: AAC34998.1.
AE009950 Genomic DNA. Translation: AAL82050.1.
RefSeqNP_579655.1. NC_003413.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1PZNX-ray2.85A/B/C/D/E/F/G1-349[»]
4A6PX-ray1.50A108-349[»]
4A6XX-ray1.55A/B108-349[»]
4A74X-ray1.48A/B108-349[»]
4A7OX-ray1.88A108-349[»]
4B2IX-ray1.30A108-349[»]
4B2LX-ray1.50A108-349[»]
4B2PX-ray1.60A108-349[»]
4B32X-ray1.50A108-349[»]
4B33X-ray1.50A108-349[»]
4B34X-ray1.55A108-349[»]
4B35X-ray1.40A108-349[»]
4B3BX-ray1.19A108-349[»]
4B3CX-ray1.90A108-349[»]
4B3DX-ray1.59A/C108-349[»]
ProteinModelPortalO74036.
SMRO74036. Positions 35-349.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING186497.PF1926.

Proteomic databases

PRIDEO74036.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL82050; AAL82050; PF1926.
GeneID1469806.
KEGGpfu:PF1926.

Phylogenomic databases

eggNOGCOG0468.
HOGENOMHOG000227426.
KOK04483.
OMASTFRTEY.
ProtClustDBPRK04301.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
HAMAPMF_00348. RadA.
InterProIPR003593. AAA+_ATPase.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR011938. DNA_recomb/repair_RadA.
IPR016467. DNA_recomb/repair_RecA-like.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PfamPF08423. Rad51. 1 hit.
[Graphical view]
PIRSFPIRSF005856. Rad51. 1 hit.
SMARTSM00382. AAA. 1 hit.
SM00278. HhH1. 2 hits.
[Graphical view]
SUPFAMSSF47794. SSF47794. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR02236. recomb_radA. 1 hit.
PROSITEPS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO74036.

Entry information

Entry nameRADA_PYRFU
AccessionPrimary (citable) accession number: O74036
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references