Reviewed, UniProtKB/Swiss-Prot O74031 (FWDC_METWO)

Names and origin

Protein names

Recommended name:
    Tungsten-containing formylmethanofuran dehydrogenase 2 subunit C
    EC=1.2.99.5
Alternative name(s):
    Tungsten-containing formylmethanofuran dehydrogenase II subunit C

Gene names

Name:

fwdC

Organism

Methanobacterium wolfei

Taxonomic identifier

145261 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of CO(2) and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR). This enzyme is oxygen-labile By similarity.
Catalytic activity	Formylmethanofuran + H(2)O + acceptor = CO(2) + methanofuran + reduced acceptor.
Cofactor	Tungsten.
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; 5,10-methenyl-H(4)MPT from CO(2): step 1/3.
Subunit structure	This enzyme is composed of six subunits fwdA, fwdB, fwdC, fwdD, fwdF and fwdG By similarity.
Induction	By growth on tungsten or molybdenum under anaerobic conditions.
Sequence similarities	Belongs to the fwdC/fmdC family.

Ontologies

Keywords
Biological process	Methanogenesis
Domain	Repeat
Ligand	Tungsten
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	formylmethanofuran dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	Tungsten-containing formylmethanofuran dehydrogenase 2 subunit C		PRO_0000144198
Regions
	Repeat	80 – 92	13	1
	Repeat	99 – 111	13	2
	Repeat	118 – 130	13	3
	Repeat	144 – 156	13	4
	Repeat	163 – 175	13	5
	Repeat	182 – 194	13	6
	Repeat	201 – 213	13	7
	Region	80 – 213	134	7 X 13 AA repeats of [GW]-X-X-M-X-X-G-X-[IL]-X-[IV]-X-G

Sequences

	Sequence		Length	Mass (Da)
	O74031-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: BB1061CBDF53061B	FASTA	270	28,660
	10 20 30 40 50 60 MSEIILTPKE QPEVPLEAPN IKPDVFAGKS IDEIKNIQIM HGNEVVKLGD FFEVSGEPAD 70 80 90 100 110 120 APEDIKIIID GDVYNTKRIG QEMTAGEIIV RGNVNMYVGA GMKGGKITVE GNAGSWAGQD 130 140 150 160 170 180 MRGGEIEILG DADDYVGSSY RGDWRGMSGG TITVHGNADN EIGEYMNGGK IIIKGDVNIM 190 200 210 220 230 240 PGIHMNNGLI IIEGNVVARA GGEMAGGTIV VKGMMQEFLA GFKYLGVEKD IELMVKNSPG 250 260 270 AFYKFEGDHA IKGAKGIVYA AVGCNGHIAP

References

[1]

"The formylmethanofuran dehydrogenase isoenzymes in Methanobacterium wolfei and Methanobacterium thermoautotrophicum: induction of the molybdenum isoenzyme by molybdate and constitutive synthesis of the tungsten isoenzyme." Hochheimer A., Hedderich R., Thauer R.K. Arch. Microbiol. 170:389-393(1998) [PubMed: 9818358] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ009688 Genomic DNA. Translation: CAA08784.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR017550. Formylmethanofuran_DH_C. IPR002489. Glu_synthase_C. [Graphical view]
Gene3D	G3DSA:2.160.20.60. Glu_synthase_C. 1 hit.
Pfam	PF01493. GXGXG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FWDC_METWO

Accession

Primary (citable) accession number: O74031

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	November 4, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)