Glutamate dehydrogenase - Thermococcus profundus

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Reviewed, UniProtKB/Swiss-Prot O74024 (DHE3_THEPR)

Last modified November 25, 2008. Version 47. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    Glutamate dehydrogenase
      Short name=GDH
    EC=1.4.1.3

Gene names

Name:	gdhA
Synonyms:	gdh

Organism

Thermococcus profundus

Taxonomic identifier

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-glutamate + H(2)O + NAD(P)(+) = 2-oxoglutarate + NH(3) + NAD(P)H.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

419

Glutamate dehydrogenase

PRO_0000182763

Regions

Nucleotide binding

7

NAD Potential

Sites

Active site

1

By similarity

Secondary structure

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419

Helix Strand Turn

Sequences

Sequence

Length

Mass (Da)

Tools

O74024-1 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 1DF3C1122E562706
Show »

419

46,700

        10         20         30         40         50         60 
MVEIDPFEMA VKQLERAAQY MDISEEALEW LKKPMRIVEV SVPIEMDDGS VKVFTGFRVQ 

        70         80         90        100        110        120 
HNWARGPTKG GIRWHPAETL STVKALATWM TWKVAVVDLP YGGGKGGIIV NPKELSEREQ 

       130        140        150        160        170        180 
ERLARAYIRA VYDVIGPWTD IPAPDVYTNP KIMGWMMDEY ETIMRRKGPA FGVITGKPLS 

       190        200        210        220        230        240 
IGGSLGRGTA TAQGAIFTIR EAAKALGIDL KGKKIAVQGY GNAGYYTAKL AKEQLGMTVV 

       250        260        270        280        290        300 
AVSDSRGGIY NPDGLDPDEV LKWKREHGSV KDFPGATNIT NEELLELEVD VLAPAAIEEV 

       310        320        330        340        350        360 
ITEKNADNIK AKIVAEVANG PVTPEADDIL REKGILQIPD FLCNAGGVTV SYFEWVQNIN 

       370        380        390        400        410 
GYYWTEEEVR EKLDKKMTKA FWEVYNTHKD KNIHMRDAAY VVAVSRVYQA MKDRGWVKK

References

[1]	"Molecular cloning, nucleotide sequence and expression in Escherichia coli of hyperthermophilic glutamate dehydrogenase gene from Thermococcus profundus." Higuchi S., Kobayashi T., Kimura K., Horikoshi K., Kudo T. J. Ferment. Bioeng. 83:405-411(1997) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

D87814 Genomic DNA. Translation: BAA28943.1.

PIR

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EUZ	X-ray	2.25	A/B/C/D/E/F	1-419	[»]

ModBase

Family and domain databases

InterPro

IPR006095. Glu/Leu/Phe/Val_DHase.
IPR006096. Glu/Leu/Phe/Val_DHase_C.
IPR006097. Glu/Leu/Phe/Val_DHase_dimer.
IPR014362. Glu_DHase.
IPR016040. NAD(P)-bd.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

PANTHER

PTHR11606:SF2. GLFV_DH. 1 hit.

Pfam

PF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]

PIRSF

PIRSF000185. Glu_DH. 1 hit.

PRINTS

PR00082. GLFDHDRGNASE.

PROSITE

PS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

ProtoNet

Entry information

Entry name

DHE3_THEPR

Accession

Primary (citable) accession number: O74024

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	November 25, 2008
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents