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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

ogt

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.UniRule annotation1 Publication

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.UniRule annotation1 Publication

Temperature dependencei

Activity is stable at 90 degrees Celsius for at least 30 minutes.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei141Nucleophile; methyl group acceptorUniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferaseUniRule annotation (EC:2.1.1.63UniRule annotation1 Publication)
Alternative name(s):
6-O-methylguanine-DNA methyltransferaseUniRule annotation
Short name:
MGMT1 PublicationUniRule annotation
O-6-methylguanine-DNA-alkyltransferaseUniRule annotation
Pk-MGMT1 Publication
Gene namesi
Name:ogtUniRule annotation
Synonyms:mgtk1 Publication
Ordered Locus Names:TK1971
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393851 – 174Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST174

Interactioni

Protein-protein interaction databases

STRINGi69014.TK1971.

Structurei

Secondary structure

1174
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 9Combined sources8
Beta strandi12 – 32Combined sources21
Helixi33 – 49Combined sources17
Helixi63 – 71Combined sources9
Helixi77 – 83Combined sources7
Helixi91 – 103Combined sources13
Helixi112 – 118Combined sources7
Helixi123 – 131Combined sources9
Turni136 – 138Combined sources3
Helixi141 – 143Combined sources3
Beta strandi144 – 146Combined sources3
Helixi157 – 166Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MGTX-ray1.80A1-174[»]
ProteinModelPortaliO74023.
SMRiO74023.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO74023.

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.UniRule annotationCurated

Phylogenomic databases

eggNOGiarCOG02724. Archaea.
COG0350. LUCA.
HOGENOMiHOG000244137.
InParanoidiO74023.
KOiK00567.
OMAiKRNPYPI.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00772. OGT. 1 hit.
InterProiIPR015236. DUF1938.
IPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR023546. MGMT.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
PF09153. DUF1938. 1 hit.
[Graphical view]
ProDomiPD068053. DUF1938. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74023-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSVEKFRVG ERVVWIGVIF SGRVQGIAFA FDRGTLMKRI HDLAEHLGKR
60 70 80 90 100
GVSISLDVQP SDYPEKVFKV LIGELDNASF LRELSFEGVT PFEKKVYEWL
110 120 130 140 150
TKNVKRGSVI TYGDLAKALN TSPRAVGGAM KRNPYPIVVP CHRVVAHDGI
160 170
GYYSSGIEEK KFLLEIEGVK EWTS
Length:174
Mass (Da):19,517
Last modified:November 1, 1998 - v1
Checksum:i1AD95478CE2EF72F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86335 Genomic DNA. Translation: BAA29044.1.
AP006878 Genomic DNA. Translation: BAD86160.1.
PIRiT44680.
RefSeqiWP_011250921.1. NC_006624.1.

Genome annotation databases

EnsemblBacteriaiBAD86160; BAD86160; TK1971.
GeneIDi3234736.
KEGGitko:TK1971.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86335 Genomic DNA. Translation: BAA29044.1.
AP006878 Genomic DNA. Translation: BAD86160.1.
PIRiT44680.
RefSeqiWP_011250921.1. NC_006624.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MGTX-ray1.80A1-174[»]
ProteinModelPortaliO74023.
SMRiO74023.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi69014.TK1971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD86160; BAD86160; TK1971.
GeneIDi3234736.
KEGGitko:TK1971.

Phylogenomic databases

eggNOGiarCOG02724. Archaea.
COG0350. LUCA.
HOGENOMiHOG000244137.
InParanoidiO74023.
KOiK00567.
OMAiKRNPYPI.

Miscellaneous databases

EvolutionaryTraceiO74023.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00772. OGT. 1 hit.
InterProiIPR015236. DUF1938.
IPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR023546. MGMT.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
PF09153. DUF1938. 1 hit.
[Graphical view]
ProDomiPD068053. DUF1938. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOGT_THEKO
AccessioniPrimary (citable) accession number: O74023
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.