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Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-650-MONOMER.
BRENDAi6.1.1.20. 5244.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase beta subunit (EC:6.1.1.20)
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name:
PheRS
Gene namesi
Name:pheT
Ordered Locus Names:PH0657
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Phenylalanine--tRNA ligase beta subunitPRO_0000127009Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

STRINGi70601.PH0657.

Structurei

Secondary structure

1
556
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 75Combined sources
Helixi8 – 158Combined sources
Helixi21 – 3010Combined sources
Beta strandi33 – 419Combined sources
Beta strandi44 – 518Combined sources
Helixi57 – 593Combined sources
Helixi62 – 7211Combined sources
Beta strandi84 – 9310Combined sources
Helixi95 – 973Combined sources
Turni98 – 1003Combined sources
Beta strandi103 – 1119Combined sources
Helixi116 – 13116Combined sources
Turni132 – 1387Combined sources
Beta strandi139 – 1468Combined sources
Helixi147 – 1493Combined sources
Beta strandi152 – 1598Combined sources
Helixi176 – 1827Combined sources
Helixi184 – 1896Combined sources
Helixi190 – 1923Combined sources
Turni193 – 1953Combined sources
Beta strandi196 – 2049Combined sources
Beta strandi209 – 2124Combined sources
Turni213 – 2153Combined sources
Beta strandi216 – 2227Combined sources
Beta strandi230 – 2389Combined sources
Helixi240 – 25516Combined sources
Turni256 – 2583Combined sources
Beta strandi260 – 2678Combined sources
Beta strandi272 – 2754Combined sources
Beta strandi281 – 2866Combined sources
Helixi287 – 2948Combined sources
Helixi300 – 30910Combined sources
Beta strandi313 – 3175Combined sources
Beta strandi320 – 3256Combined sources
Helixi335 – 34612Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CXIX-ray1.94A/B/C1-348[»]
ProteinModelPortaliO73984.
SMRiO73984. Positions 2-348.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO73984.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini278 – 35376B5Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi135 – 1384Poly-Arg

Sequence similaritiesi

Contains 1 B5 domain.Curated

Phylogenomic databases

eggNOGiarCOG00412. Archaea.
COG0072. LUCA.
HOGENOMiHOG000105094.
KOiK01890.
OMAiERPYVTG.

Family and domain databases

Gene3Di3.30.56.20. 1 hit.
HAMAPiMF_00284. Phe_tRNA_synth_beta2. 1 hit.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR004531. Phe-tRNA-synth_IIc_bsu_arc.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR022918. Phe_tRNA_ligase_beta2_bac/arc.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 2 hits.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00471. pheT_arch. 1 hit.
PROSITEiPS51483. B5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O73984-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKFDVSKSD LERLIGRSFS IEEWEDLVLY AKCELDDVWE ENGKVYFKLD
60 70 80 90 100
SKDTNRPDLW SAEGVARQIK WALGIEKGLP KYEVKKSNVT VYVDEKLKDI
110 120 130 140 150
RPYGVYAIVE GLRLDEDSLS QMIQLQEKIA LTFGRRRREV AIGIFDFDKI
160 170 180 190 200
KPPIYYKAAE KTEKFAPLGY KEEMTLEEIL EKHEKGREYG HLIKDKQFYP
210 220 230 240 250
LLIDSEGNVL SMPPIINSEF TGRVTTDTKN VFIDVTGWKL EKVMLALNVM
260 270 280 290 300
VTALAERGGK IRSVRVVYKD FEIETPDLTP KEFEVELDYI RKLSGLELND
310 320 330 340 350
GEIKELLEKM MYEVEISRGR AKLKYPAFRD DIMHARDILE DVLIAYGYNN
360 370 380 390 400
IEPEEPKLAV QGRGDPFKDF EDAIRDLMVG FGLQEVMTFN LTNKEVQFKK
410 420 430 440 450
MNIPEEEIVE IANPISQRWS ALRKWILPSL MEFLSNNTHE EYPQRIFEVG
460 470 480 490 500
LATLIDESRE TKTVSEPKLA VALAGTGYTF TNAKEILDAL MRHLGFEYEI
510 520 530 540 550
EEVEHGSFIP GRAGKIIVNG RDIGIIGEVH PQVLENWNIE VPVVAFEIFL

RPLYRH
Length:556
Mass (Da):64,195
Last modified:November 1, 1998 - v1
Checksum:iD3DB228E4AB7CBC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29748.1.
PIRiB71111.
RefSeqiWP_010884752.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29748; BAA29748; BAA29748.
GeneIDi1442987.
KEGGipho:PH0657.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29748.1.
PIRiB71111.
RefSeqiWP_010884752.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CXIX-ray1.94A/B/C1-348[»]
ProteinModelPortaliO73984.
SMRiO73984. Positions 2-348.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0657.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA29748; BAA29748; BAA29748.
GeneIDi1442987.
KEGGipho:PH0657.

Phylogenomic databases

eggNOGiarCOG00412. Archaea.
COG0072. LUCA.
HOGENOMiHOG000105094.
KOiK01890.
OMAiERPYVTG.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-650-MONOMER.
BRENDAi6.1.1.20. 5244.

Miscellaneous databases

EvolutionaryTraceiO73984.

Family and domain databases

Gene3Di3.30.56.20. 1 hit.
HAMAPiMF_00284. Phe_tRNA_synth_beta2. 1 hit.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR004531. Phe-tRNA-synth_IIc_bsu_arc.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR022918. Phe_tRNA_ligase_beta2_bac/arc.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 2 hits.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00471. pheT_arch. 1 hit.
PROSITEiPS51483. B5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYFB_PYRHO
AccessioniPrimary (citable) accession number: O73984
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: November 1, 1998
Last modified: November 11, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.