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O73984 (SYFB_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phenylalanine--tRNA ligase beta chain
EC=6.1.1.20
Alternative name(s):
Phenylalanyl-tRNA synthetase beta chain
Short name=PheRS
Gene names
Name:pheT
Ordered Locus Names:PH0657
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00284

Subunit structure

Tetramer of two alpha and two beta chains By similarity.

Subcellular location

Cytoplasm HAMAP MF_00284.

Sequence similarities

Belongs to the phenylalanyl-tRNA synthetase beta chain family. Type 2 subfamily.

Contains 1 B5 domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processphenylalanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

phenylalanine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556Phenylalanine--tRNA ligase beta chain HAMAP MF_00284
PRO_0000127009

Regions

Domain278 – 35376B5
Compositional bias135 – 1384Poly-Arg HAMAP MF_00284

Secondary structure

1 ........................................................... 556
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O73984 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: D3DB228E4AB7CBC0

FASTA55664,195
        10         20         30         40         50         60 
MPKFDVSKSD LERLIGRSFS IEEWEDLVLY AKCELDDVWE ENGKVYFKLD SKDTNRPDLW 

        70         80         90        100        110        120 
SAEGVARQIK WALGIEKGLP KYEVKKSNVT VYVDEKLKDI RPYGVYAIVE GLRLDEDSLS 

       130        140        150        160        170        180 
QMIQLQEKIA LTFGRRRREV AIGIFDFDKI KPPIYYKAAE KTEKFAPLGY KEEMTLEEIL 

       190        200        210        220        230        240 
EKHEKGREYG HLIKDKQFYP LLIDSEGNVL SMPPIINSEF TGRVTTDTKN VFIDVTGWKL 

       250        260        270        280        290        300 
EKVMLALNVM VTALAERGGK IRSVRVVYKD FEIETPDLTP KEFEVELDYI RKLSGLELND 

       310        320        330        340        350        360 
GEIKELLEKM MYEVEISRGR AKLKYPAFRD DIMHARDILE DVLIAYGYNN IEPEEPKLAV 

       370        380        390        400        410        420 
QGRGDPFKDF EDAIRDLMVG FGLQEVMTFN LTNKEVQFKK MNIPEEEIVE IANPISQRWS 

       430        440        450        460        470        480 
ALRKWILPSL MEFLSNNTHE EYPQRIFEVG LATLIDESRE TKTVSEPKLA VALAGTGYTF 

       490        500        510        520        530        540 
TNAKEILDAL MRHLGFEYEI EEVEHGSFIP GRAGKIIVNG RDIGIIGEVH PQVLENWNIE 

       550 
VPVVAFEIFL RPLYRH

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000001 Genomic DNA. Translation: BAA29748.1.
PIRB71111.
RefSeqNP_142611.1. NC_000961.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CXIX-ray1.94A/B/C1-348[»]
ProteinModelPortalO73984.
SMRO73984. Positions 2-348.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBPYRT00000000573; EBPYRP00000000573; EBPYRG00000000573.
GeneID1442987.
GenomeReviewsGene locus PH0657 in contig BA000001_GR.
KEGGpho:PH0657.
NMPDRfig|70601.1.peg.640.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000023212.
HOGENOMHBG497667.
OMALLTRMCL.
PhylomeDBO73984.
ProtClustDBPRK09616.

Enzyme and pathway databases

BioCycPHOR70601:PH0657-MONOMER.

Family and domain databases

HAMAPMF_00284. Phe_tRNA_synth_beta2.
[Tree]
InterProIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR004531. Phe-tRNA-synth_IIc_bsu_arc.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR022918. Phe_tRNA_synth_beta2_bac/arc.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
Gene3DG3DSA:3.30.56.20. B5. 1 hit.
KOK01890.
PfamPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
SMARTSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
[Graphical view]
SUPFAMSSF56037. B3_4. 1 hit.
SSF46955. Putativ_DNA_bind. 2 hits.
TIGRFAMsTIGR00471. PheT_arch. 1 hit.
PROSITEPS51483. B5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYFB_PYRHO
AccessionPrimary (citable) accession number: O73984
Entry history
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: November 1, 1998
Last modified: January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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