Reviewed, UniProtKB/Swiss-Prot O73955 (KATG_HALSA)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase
      Short name=CP
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase
    Hydroperoxidase

Gene names

Name:	katG
Synonyms:	perA
Ordered Locus Names:	VNG_6294G

Encoded on

Plasmid pNRC200 Ref.2

Organism

Halobacterium salinarium (Halobacterium halobium) [Complete proteome] [HAMAP]

Taxonomic identifier

2242 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	720 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.
Catalytic activity	2 H(2)O(2) = O(2) + 2 H(2)O. Donor + H(2)O(2) = oxidized donor + 2 H(2)O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 720	720	Catalase-peroxidase		PRO_0000055581
Sites
	Active site	83	1	Proton acceptor By similarity
	Metal binding	248	1	Iron (heme axial ligand) By similarity
	Site	79	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	82 ↔ 207		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-233) By similarity
	Cross-link	207 ↔ 233		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-82) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O73955-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: FB88823BCD3CB2F9	FASTA	720	80,477
	10 20 30 40 50 60 MENEDHNFGT SDWWPNQLDL EILDQNSQQV DPYGEDFDYA EAFEDLDLAA VKDDLEEMMT 70 80 90 100 110 120 DSKDWWPADY GHYGPLFIRM AWHSAGTYRT FDGRGGAAGG RQRLPPVDSW PDNVNLDKAR 130 140 150 160 170 180 RLLWPIKQKY GRKLSWGDLI ILAGNVALES MGFETYGFAG GRKDDYTPDE AVDWGPEDEW 190 200 210 220 230 240 ETTSGDRFDA DGSLKWPLGN TVMGLIYVNP EGPNGEPDLE GSAKNIRESF GKMAMNDKET 250 260 270 280 290 300 VALIAGGHTF GKVHGADDPE ENVGAEPAAA PIEKQGLGWE NEFGEGKGPD TITSGIEGPW 310 320 330 340 350 360 NTTPTQWDMS YVDNLLEYEW EPEKGPGGAW QWTTKSGELN ESAPGVQDPT DTEDVMMLTT 370 380 390 400 410 420 DVALKDDPDY REVLETFQEN PREFQQSFSK AWYKLIHRDM GPSERFLGPE VPEETMIWQD 430 440 450 460 470 480 PLPDADYDLV DDEAVAALKS ELLESELSIP QLVKTAWASA STYRDSDKRG GANGARIRLE 490 500 510 520 530 540 PQRSWEVNEP EQLEAALSTY EDIQAEFNDA RSDDMRVSLA DLIVLGGNAA IEQAAADAGY 550 560 570 580 590 600 DVDVPFEPGR TDATPEQTDV ESFEALKPKA DGFRNYLGDD AEREPEELLV DKAELLNLTA 610 620 630 640 650 660 DDMTVLVGGL RALGVTHGDS ELGIFTDQPG TLTNDFFTTL LDMDYEWEAA SEDREVFELR 670 680 690 700 710 720 DRETGDVEWT GSRVDLLFGS NTRLRAIAEV YGSDADEELF VQDFVDTWSE VMKLDRFDLE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, sequencing analysis and expression of the catalase-peroxidase gene from Halobacterium salinarum." Long S., Salin M.L. DNA Seq. 12:39-51(2001) [PubMed: 11702717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF069761 Genomic DNA. Translation: AAC23534.1. AE004438 Genomic DNA. Translation: AAG20931.1.
PIR	T44562.
RefSeq	NP_395796.1.
3D structure databases
HSSP	HSSP built from PDB template 1ITK based on UniProtKB O59651.
ModBase	Search...
Protein family/group databases
PeroxiBase	2441. HAspCP01.
Genome annotation databases
GeneID	1449179.
GenomeReviews	Gene locus VNG_6294G in contig AE004438_GR.
KEGG	hal:VNG6294G.
NMPDR	fig|64091.1.peg.2480.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O73955.
Enzyme and pathway databases
BioCyc	HSP64091:VNG6294G-MON.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_HALSA

Accession

Primary (citable) accession number: O73955
Secondary accession number(s): Q9HHP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1998
Last modified:	November 25, 2008
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)