Catalase-peroxidase - Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Hydroperoxidase
Peroxidase/catalase

Gene names

Name:	katG
Synonyms:	perA
Ordered Locus Names:	VNG_6294G

Encoded on

Plasmid pNRC200 Ref.2

Organism

Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)

Taxonomic identifier

64091 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	720 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. Ref.1
Catalytic activity	Donor + H₂O₂ = oxidized donor + 2 H₂O. HAMAP MF_01961 2 H₂O₂ = O₂ + 2 H₂O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Plasmid Reference proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 720

720

Catalase-peroxidase HAMAP MF_01961

PRO_0000055581

Sites

Active site

83

1

Proton acceptor By similarity

Metal binding

248

1

Iron (heme axial ligand) By similarity

Site

79

1

Transition state stabilizer By similarity

Amino acid modifications

Cross-link

82 ↔ 207

Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-233) By similarity

Cross-link

207 ↔ 233

Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-82) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

O73955 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: FB88823BCD3CB2F9
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FASTA

720

80,477

        10         20         30         40         50         60 
MENEDHNFGT SDWWPNQLDL EILDQNSQQV DPYGEDFDYA EAFEDLDLAA VKDDLEEMMT 

        70         80         90        100        110        120 
DSKDWWPADY GHYGPLFIRM AWHSAGTYRT FDGRGGAAGG RQRLPPVDSW PDNVNLDKAR 

       130        140        150        160        170        180 
RLLWPIKQKY GRKLSWGDLI ILAGNVALES MGFETYGFAG GRKDDYTPDE AVDWGPEDEW 

       190        200        210        220        230        240 
ETTSGDRFDA DGSLKWPLGN TVMGLIYVNP EGPNGEPDLE GSAKNIRESF GKMAMNDKET 

       250        260        270        280        290        300 
VALIAGGHTF GKVHGADDPE ENVGAEPAAA PIEKQGLGWE NEFGEGKGPD TITSGIEGPW 

       310        320        330        340        350        360 
NTTPTQWDMS YVDNLLEYEW EPEKGPGGAW QWTTKSGELN ESAPGVQDPT DTEDVMMLTT 

       370        380        390        400        410        420 
DVALKDDPDY REVLETFQEN PREFQQSFSK AWYKLIHRDM GPSERFLGPE VPEETMIWQD 

       430        440        450        460        470        480 
PLPDADYDLV DDEAVAALKS ELLESELSIP QLVKTAWASA STYRDSDKRG GANGARIRLE 

       490        500        510        520        530        540 
PQRSWEVNEP EQLEAALSTY EDIQAEFNDA RSDDMRVSLA DLIVLGGNAA IEQAAADAGY 

       550        560        570        580        590        600 
DVDVPFEPGR TDATPEQTDV ESFEALKPKA DGFRNYLGDD AEREPEELLV DKAELLNLTA 

       610        620        630        640        650        660 
DDMTVLVGGL RALGVTHGDS ELGIFTDQPG TLTNDFFTTL LDMDYEWEAA SEDREVFELR 

       670        680        690        700        710        720 
DRETGDVEWT GSRVDLLFGS NTRLRAIAEV YGSDADEELF VQDFVDTWSE VMKLDRFDLE

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, sequencing analysis and expression of the catalase-peroxidase gene from Halobacterium salinarum." Long S., Salin M.L. DNA Seq. 12:39-51(2001) [PubMed: 11702717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF069761 Genomic DNA. Translation: AAC23534.1. AE004438 Genomic DNA. Translation: AAG20931.1.
PIR	T44562.
RefSeq	NP_395796.1. NC_002608.1.
3D structure databases
ProteinModelPortal	O73955.
SMR	O73955. Positions 12-720.
ModBase	Search...
Protein family/group databases
PeroxiBase	2441. HAspCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1449179.
GenomeReviews	Gene locus VNG_6294G in contig AE004438_GR.
KEGG	hal:VNG6294G.
NMPDR	fig\|64091.1.peg.2480.
Phylogenomic databases
HOGENOM	HBG285610.
OMA	KRHAPSM.
PhylomeDB	O73955.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	HSP64091:VNG6294G-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_HALSA

Accession

Primary (citable) accession number: O73955
Secondary accession number(s): Q9HHP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1998
Last modified:	December 14, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents