O73951 (O73951_9EURY) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Endo-beta-1,3-glucanase EMBL AAC25554.2

Gene names

Name:

lamA EMBL AAC25554.2

Organism

Pyrococcus furiosus EMBL AAC25554.2

Taxonomic identifier

2261 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

Ontologies

Keywords
Domain	Signal EMBL AAC25554.2
Ligand	Calcium PDB 2VY0 Metal-binding PDB 2VY0
Technical term	3D-structure PDB 2VY0
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential EMBL AAC25554.2
	Chain	21 – 297	277	endo-beta-1,3-glucanase EMBL AAC25554.2		PRO_5000053327
Sites
	Metal binding	53	1	Calcium; via carbonyl oxygen PDB 2VY0
	Metal binding	55	1	Calcium PDB 2VY0
	Metal binding	97	1	Calcium; via carbonyl oxygen PDB 2VY0
	Metal binding	287	1	Calcium PDB 2VY0

Sequences

	Sequence		Length	Mass (Da)
	O73951 [UniParc]. Last modified May 24, 2005. Version 2. Checksum: BE5E37C051379D46	FASTA	297	33,842
	10 20 30 40 50 60 MKKEALLFLS LIFLVFVSGC IHHSTNQQLS SKQQVPEVIE IDGKQWRLIW HDEFEGSEVN 70 80 90 100 110 120 KEYWTFEKGN GIAYGIPGWG NGELEYYTEN NTYIVNGTLV IEARKEIITD PNEGTFLYTS 130 140 150 160 170 180 SRLKTEGKVE FSPPVVVEAR IKLPKGKGLW PAFWMLGSNI REVGWPNCGE IDIMEFLGHE 190 200 210 220 230 240 PRTIHGTVHG PGYSGSKGIT RAYTLPEGVP DFTEDFHVFG IVWYPDKIKW YVDGTFYHEV 250 260 270 280 290 TKEQVEAMGY EWVFDKPFYI ILNLAVGGYW PGNPDATTPF PAKMVVDYVR VYSFVSG

References

[1]	"Molecular and biochemical characterization of an endo-beta-1,3-glucanase of the hyperthermophilic archaeon Pyrococcus furiosus." Gueguen Y., Voorhorst W.G., van der Oost J., de Vos W.M. J. Biol. Chem. 272:31258-31264(1997) [PubMed: 9395451] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: DSM 3638 EMBL AAC25554.2.
[2]	"Transcriptional regulation in the hyperthermophilic archaeon Pyrococcus furiosus: coordinated expression of divergently oriented genes in response to beta-linked glucose polymers." Voorhorst W.G., Gueguen Y., Geerling A.C.M., Schut G., Dahlke I., Thomm M., van der Oost J., de Vos W.M. J. Bacteriol. 181:3777-3783(1999) [PubMed: 10368153] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: DSM 3638 EMBL AAC25554.2.
[3]	van der Oost J. Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: DSM 3638 EMBL AAC25554.2.
[4]	"Crystal structure of a family 16 endoglucanase from the hyperthermophile Pyrococcus furiosus--structural basis of substrate recognition." Ilari A., Fiorillo A., Angelaccio S., Florio R., Chiaraluce R., van der Oost J., Consalvi V. FEBS J. 276:1048-1058(2009) [PubMed: 19154353] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.16 ANGSTROMS) OF 35-297 IN COMPLEX WITH CALCIUM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF013169 Genomic DNA. Translation: AAC25554.2.

RefSeq

NP_577805.1. NC_003413.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VY0	X-ray	2.16	A/B	35-297	[»]

ProteinModelPortal

O73951.

ModBase

Search...

Protein family/group databases

CAZy

GH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBPYRT00000004093; EBPYRP00000003954; EBPYRG00000004093.

GeneID

1467905.

NMPDR

fig|186497.1.peg.76.

Phylogenomic databases

HOGENOM

HBG696112.

ProtClustDB

CLSK2518674.

Family and domain databases

InterPro

IPR008985. ConA-like_lec_gl.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
[Graphical view]

Gene3D

G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.

Pfam

PF00722. Glyco_hydro_16. 1 hit.
[Graphical view]

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.

ProtoNet

Search...

Entry information

Entry name

O73951_9EURY

Accession

Primary (citable) accession number: O73951
Secondary accession number(s): Q8U4K8

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1998
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)