O73948 (RADA_METVO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA repair and recombination protein radA

Gene names

Name:

radA

Organism

Methanococcus voltae

Taxonomic identifier

2188 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules By similarity. HAMAP MF_00348
Sequence similarities	Belongs to the eukaryotic recA-like protein family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	3D-structure
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-dependent ATPase activity Inferred from electronic annotation. Source: InterPro damaged DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 322

322

DNA repair and recombination protein radA HAMAP MF_00348

PRO_0000150099

Regions

Nucleotide binding

105 – 112

8

ATP Potential

Secondary structure

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322

Helix Strand Turn

Details...

Helix

13 – 21

9

Helix

27 – 31

5

Helix

35 – 39

5

Helix

46 – 59

14

Helix

68 – 74

7

Helix

85 – 90

6

Turn

91 – 93

3

Beta strand

94 – 96

3

Beta strand

99 – 106

8

Helix

111 – 121

11

Helix

125 – 127

3

Turn

132 – 134

3

Helix

137 – 140

4

Beta strand

141 – 152

12

Helix

156 – 165

10

Helix

170 – 175

6

Beta strand

177 – 181

5

Helix

185 – 193

9

Helix

195 – 200

6

Beta strand

204 – 211

8

Helix

215 – 220

6

Helix

227 – 247

21

Beta strand

251 – 257

7

Beta strand

283 – 290

8

Beta strand

295 – 302

8

Beta strand

304 – 306

3

Beta strand

309 – 316

8

Beta strand

319 – 321

3

Sequences

	Sequence		Length	Mass (Da)
	O73948 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 8A9F5EFB927344B6	FASTA	322	35,189
	10 20 30 40 50 60 MSDNLTDLPG VGPSTAEKLV EAGYIDFMKI ATATVGELTD IEGISEKAAA KMIMGARDLC 70 80 90 100 110 120 DLGFKSGIDL LKQRSTVWKL STSSSELDSV LGGGLESQSV TEFAGVFGSG KTQIMHQSCV 130 140 150 160 170 180 NLQNPEFLFY DEEAVSKGEV AQPKAVYIDT EGTFRPERIM QMAEHAGIDG QTVLDNTFVA 190 200 210 220 230 240 RAYNSDMQML FAEKIEDLIQ EGNNIKLVVI DSLTSTFRNE YTGRGKLAER QQKLGRHMAT 250 260 270 280 290 300 LNKLADLFNC VVLVTNQVSA KPDAFFGMAE QAIGGHIVGH AATFRFFVRK GKGDKRVAKL 310 320 YDSPHLPDAE AIFRITEKGI QD

References

[1]	"The RadA protein from the archaeon Methanococcus voltae: a functional homolog of eukaryotic Rad51." Reich C.I., Buldak G.L., McNeil L.K. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33273 / DSM 1537 / NBRC 100457 / OCM 70 / PS.
[2]	"Diversity of radA genes from cultured and uncultured archaea: comparative analysis of putative RadA proteins and their use as a phylogenetic marker." Sandler S.J., Hugenholtz P., Schleper C., DeLong E.F., Pace N.R., Clark A.J. J. Bacteriol. 181:907-915(1999) [PubMed: 9922255] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 112-254.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF008421 Genomic DNA. Translation: AAC23499.1.
AF090200 Genomic DNA. Translation: AAD16066.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1T4G	X-ray	2.00	A	3-322	[»]
1XU4	X-ray	2.40	A	3-322	[»]
2B21	X-ray	2.40	A	3-322	[»]
2F1H	X-ray	2.70	A	3-322	[»]
2F1I	X-ray	2.90	A	3-322	[»]
2F1J	X-ray	2.30	A	3-322	[»]
2FPK	X-ray	2.10	A	3-322	[»]
2FPL	X-ray	2.30	A	3-322	[»]
2FPM	X-ray	2.00	A	3-322	[»]
2GDJ	X-ray	2.50	A	63-322	[»]
2I1Q	X-ray	1.90	A	3-322	[»]
3FYH	X-ray	1.90	A	3-322	[»]
3NTU	X-ray	1.90	A	5-322	[»]

ProteinModelPortal

O73948.

SMR

O73948. Positions 5-322.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29120N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

HAMAP

MF_00348. RadA.
[Tree]

InterPro

IPR003593. ATPase_AAA+_core.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR011938. DNA_recomb/repair_RadA.
IPR016467. DNA_recomb/repair_RecA-like.
IPR020588. DNA_recomb_RecA/RadB_ATP-bd.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]

PANTHER

PTHR22942:SF11. PTHR22942:SF11. 1 hit.

Pfam

PF08423. Rad51. 1 hit.
[Graphical view]

PIRSF

PIRSF005856. Rad51. 1 hit.

SMART

SM00382. AAA. 1 hit.
SM00278. HhH1. 2 hits.
[Graphical view]

SUPFAM

SSF47794. Rad51_N. 1 hit.

TIGRFAMs

TIGR02236. Recomb_radA. 1 hit.

PROSITE

PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RADA_METVO

Accession

Primary (citable) accession number: O73948

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 1, 1998
Last modified:	October 19, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program