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Protein

DNA polymerase sliding clamp

Gene

pcn

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication. Unlike its eukaryotic paralog, loads on circular DNA without the replication factor C (RFC) clamp loader, although RFC greatly increases loading efficiency. Stimulates the ATPase activity of replication factor C (RFC) in the presence of ssDNA. Stimulates the helicase activity of Hel308 and may alter its substrate specificity.3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase sliding clampUniRule annotation
Alternative name(s):
Proliferating cell nuclear antigen homologUniRule annotation
Short name:
PCNAUniRule annotation
Gene namesi
Name:pcnUniRule annotation
Ordered Locus Names:PF0983
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73M → L: No observable effect. 1 Publication1
Mutagenesisi143 – 147DAVKD → AAVKA: No homotrimer formation. Stimulates ATPase activity of RFC, no stimulation of DNA synthesis by Pol I in presence and absence of RFC. Crystallizes as tail-to-tail homodimers. 1 Publication5
Mutagenesisi143D → A: Small amount of homotrimer in solution. Stimulates ATPase activity of RFC and DNA synthesis by Pol I in presence and absence of RFC. Crystallizes as tail-to-tail homodimers. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492061 – 249DNA polymerase sliding clampAdd BLAST249

Proteomic databases

PRIDEiO73947

Interactioni

Subunit structurei

Homotrimer which circularizes head-to-tail (head is a N-terminus, tail is at C-terminus) to form a toroid. RFC opens the toroid so it can load on DNA. Interacts with both Pol I (pol) and Pol II (polB-polC), with Hel308 (hjm) and with Hjc. Interaction with the C-terminal PIP-box of RfcL may stabilize the toroidal structure.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
polBP814122EBI-15762053,EBI-15906191

Protein-protein interaction databases

DIPiDIP-48777N
IntActiO73947, 2 interactors
STRINGi186497.PF0983

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi10 – 20Combined sources11
Beta strandi25 – 31Combined sources7
Beta strandi33 – 41Combined sources9
Beta strandi45 – 54Combined sources10
Helixi55 – 57Combined sources3
Beta strandi58 – 65Combined sources8
Beta strandi67 – 72Combined sources6
Helixi73 – 80Combined sources8
Beta strandi88 – 93Combined sources6
Beta strandi95 – 114Combined sources20
Beta strandi132 – 137Combined sources6
Helixi138 – 148Combined sources11
Turni149 – 151Combined sources3
Beta strandi153 – 160Combined sources8
Beta strandi163 – 169Combined sources7
Beta strandi170 – 172Combined sources3
Beta strandi174 – 180Combined sources7
Turni181 – 184Combined sources4
Beta strandi185 – 193Combined sources9
Beta strandi195 – 200Combined sources6
Helixi201 – 208Combined sources8
Beta strandi216 – 221Combined sources6
Beta strandi227 – 233Combined sources7
Turni234 – 236Combined sources3
Beta strandi237 – 243Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GE8X-ray2.10A1-249[»]
1ISQX-ray2.30A1-249[»]
1IZ4X-ray2.00A1-249[»]
1IZ5X-ray1.80A/B1-249[»]
3A2FX-ray2.67B2-249[»]
5AUJX-ray2.50A1-249[»]
ProteinModelPortaliO73947
SMRiO73947
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO73947

Family & Domainsi

Sequence similaritiesi

Belongs to the PCNA family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00488 Archaea
COG0592 LUCA
HOGENOMiHOG000222371
KOiK04802
OMAiKCANSDD
OrthoDBiPOG093Z0814

Family and domain databases

HAMAPiMF_00317 DNApol_clamp_arch, 1 hit
InterProiView protein in InterPro
IPR000730 Pr_cel_nuc_antig
IPR022649 Pr_cel_nuc_antig_C
IPR022659 Pr_cel_nuc_antig_CS
IPR022648 Pr_cel_nuc_antig_N
PANTHERiPTHR11352:SF0 PTHR11352:SF0, 1 hit
PfamiView protein in Pfam
PF02747 PCNA_C, 1 hit
PF00705 PCNA_N, 1 hit
PRINTSiPR00339 PCNACYCLIN
TIGRFAMsiTIGR00590 pcna, 1 hit
PROSITEiView protein in PROSITE
PS01251 PCNA_1, 1 hit

Sequencei

Sequence statusi: Complete.

O73947-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFEIVFEGA KEFAQLIDTA SKLIDEAAFK VTEDGISMRA MDPSRVVLID
60 70 80 90 100
LNLPSSIFSK YEVVEPETIG VNMDHLKKIL KRGKAKDTLI LKKGEENFLE
110 120 130 140 150
ITIQGTATRT FRVPLIDVEE MEVDLPELPF TAKVVVLGEV LKDAVKDASL
160 170 180 190 200
VSDSIKFIAR ENEFIMKAEG ETQEVEIKLT LEDEGLLDIE VQEETKSAYG
210 220 230 240
VSYLSDMVKG LGKADEVTIK FGNEMPMQME YYIRDEGRLT FLLAPRVEE
Length:249
Mass (Da):28,005
Last modified:February 21, 2001 - v2
Checksum:iB6932127B001FA74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017486 Genomic DNA Translation: BAA33020.2
AE009950 Genomic DNA Translation: AAL81107.1
RefSeqiWP_011012120.1, NC_003413.1

Genome annotation databases

EnsemblBacteriaiAAL81107; AAL81107; PF0983
GeneIDi1468848
KEGGipfu:PF0983
PATRICifig|186497.12.peg.1042

Similar proteinsi

Entry informationi

Entry nameiPCNA_PYRFU
AccessioniPrimary (citable) accession number: O73947
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 21, 2001
Last modified: March 28, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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