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Protein

ATP-dependent DNA helicase Hel308

Gene

hel308

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks. Unwinds the lagging strand from forked DNA structures in a 3'-5' direction. PCNA, the DNA polymerase sliding clamp subunit, stimulates the helicase activity, and may alter substrate specificity. Unwinds branched DNA (Holliday junctions) in an ATP-dependent fashion; ss- and dsDNA stimulate ATPase to the greatest extent, although it preferentially binds DNA with a single-stranded region. Processes a RecA-mediated recombination intermediate between gapped circular and homologus linear dsDNA.UniRule annotation2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Cofactori

Mg2+1 Publication, Zn2+1 PublicationNote: Divalent cations, Mg2+ and Zn2+ are best.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei28ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 53ATP8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.4.12. 5243.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase Hel308UniRule annotation (EC:3.6.4.12UniRule annotation)
Alternative name(s):
ATP-dependent Holliday junction unwindase Hjm
Holliday junction migration DNA helicase
Gene namesi
Name:hel308UniRule annotation
Synonyms:hjm
Ordered Locus Names:PF0677
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi52K → A: No ATPase activity. 1 Publication1
Mutagenesisi145D → A: No ATPase activity. 1 Publication1
Mutagenesisi146E → A: No ATPase activity. 1 Publication1
Mutagenesisi701 – 720Missing : No binding to PCNA. 1 PublicationAdd BLAST20

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001021101 – 720ATP-dependent DNA helicase Hel308Add BLAST720

Proteomic databases

PRIDEiO73946.

Expressioni

Inductioni

Constitutively expressed (at protein level).1 Publication

Interactioni

Subunit structurei

Monomer. Interacts with PCNA.UniRule annotation2 Publications

Protein-protein interaction databases

STRINGi186497.PF0677.

Structurei

Secondary structure

1720
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 5Combined sources3
Helixi10 – 18Combined sources9
Helixi26 – 32Combined sources7
Turni33 – 35Combined sources3
Helixi36 – 38Combined sources3
Beta strandi41 – 45Combined sources5
Helixi48 – 50Combined sources3
Helixi52 – 67Combined sources16
Beta strandi69 – 74Combined sources6
Helixi78 – 80Combined sources3
Helixi81 – 87Combined sources7
Helixi89 – 94Combined sources6
Beta strandi98 – 101Combined sources4
Helixi110 – 114Combined sources5
Beta strandi116 – 120Combined sources5
Helixi122 – 131Combined sources10
Helixi136 – 138Combined sources3
Beta strandi139 – 145Combined sources7
Helixi147 – 151Combined sources5
Turni153 – 155Combined sources3
Helixi156 – 166Combined sources11
Beta strandi170 – 176Combined sources7
Helixi182 – 188Combined sources7
Beta strandi191 – 195Combined sources5
Beta strandi200 – 209Combined sources10
Beta strandi212 – 215Combined sources4
Beta strandi220 – 222Combined sources3
Helixi228 – 235Combined sources8
Beta strandi240 – 243Combined sources4
Helixi247 – 261Combined sources15
Helixi262 – 264Combined sources3
Helixi267 – 278Combined sources12
Helixi284 – 293Combined sources10
Turni294 – 296Combined sources3
Beta strandi297 – 300Combined sources4
Helixi306 – 317Combined sources12
Beta strandi323 – 326Combined sources4
Beta strandi328 – 330Combined sources3
Helixi331 – 333Combined sources3
Beta strandi338 – 343Combined sources6
Beta strandi346 – 348Combined sources3
Beta strandi351 – 353Combined sources3
Helixi359 – 366Combined sources8
Turni372 – 374Combined sources3
Beta strandi376 – 383Combined sources8
Beta strandi385 – 387Combined sources3
Helixi389 – 396Combined sources8
Helixi411 – 424Combined sources14
Helixi430 – 438Combined sources9
Helixi441 – 445Combined sources5
Helixi450 – 465Combined sources16
Beta strandi468 – 471Combined sources4
Beta strandi477 – 479Combined sources3
Helixi481 – 489Combined sources9
Helixi493 – 508Combined sources16
Helixi512 – 520Combined sources9
Helixi532 – 534Combined sources3
Helixi535 – 545Combined sources11
Helixi546 – 548Combined sources3
Turni554 – 557Combined sources4
Helixi561 – 581Combined sources21
Helixi586 – 593Combined sources8
Helixi597 – 621Combined sources25
Helixi624 – 626Combined sources3
Helixi627 – 639Combined sources13
Helixi643 – 648Combined sources6
Beta strandi651 – 653Combined sources3
Helixi656 – 663Combined sources8
Turni664 – 666Combined sources3
Helixi670 – 674Combined sources5
Helixi678 – 682Combined sources5
Helixi689 – 699Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZJ2X-ray2.40A1-720[»]
2ZJ5X-ray2.40A1-720[»]
2ZJ8X-ray2.00A1-720[»]
2ZJAX-ray2.70A1-720[»]
ProteinModelPortaliO73946.
SMRiO73946.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO73946.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 197Helicase ATP-bindingUniRule annotationAdd BLAST165
Domaini229 – 422Helicase C-terminalUniRule annotationAdd BLAST194

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi145 – 148DEAH box4

Sequence similaritiesi

Belongs to the helicase family. Hel308 subfamily.UniRule annotation
Contains 1 helicase ATP-binding domain.UniRule annotation
Contains 1 helicase C-terminal domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00553. Archaea.
COG1204. LUCA.
HOGENOMiHOG000227028.
KOiK03726.
OMAiDEIHYLG.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
HAMAPiMF_00442. Helicase_Hel308. 1 hit.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR022965. Helicase_Hel308.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00278. HhH1. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O73946-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVDELRVDE RIKSTLKERG IESFYPPQAE ALKSGILEGK NALISIPTAS
60 70 80 90 100
GKTLIAEIAM VHRILTQGGK AVYIVPLKAL AEEKFQEFQD WEKIGLRVAM
110 120 130 140 150
ATGDYDSKDE WLGKYDIIIA TAEKFDSLLR HGSSWIKDVK ILVADEIHLI
160 170 180 190 200
GSRDRGATLE VILAHMLGKA QIIGLSATIG NPEELAEWLN AELIVSDWRP
210 220 230 240 250
VKLRRGVFYQ GFVTWEDGSI DRFSSWEELV YDAIRKKKGA LIFVNMRRKA
260 270 280 290 300
ERVALELSKK VKSLLTKPEI RALNELADSL EENPTNEKLA KAIRGGVAFH
310 320 330 340 350
HAGLGRDERV LVEENFRKGI IKAVVATPTL SAGINTPAFR VIIRDIWRYS
360 370 380 390 400
DFGMERIPII EVHQMLGRAG RPKYDEVGEG IIVSTSDDPR EVMNHYIFGK
410 420 430 440 450
PEKLFSQLSN ESNLRSQVLA LIATFGYSTV EEILKFISNT FYAYQRKDTY
460 470 480 490 500
SLEEKIRNIL YFLLENEFIE ISLEDKIRPL SLGIRTAKLY IDPYTAKMFK
510 520 530 540 550
DKMEEVVKDP NPIGIFHLIS LTPDITPFNY SKREFERLEE EYYEFKDRLY
560 570 580 590 600
FDDPYISGYD PYLERKFFRA FKTALVLLAW INEVPEGEIV EKYSVEPGDI
610 620 630 640 650
YRIVETAEWL VYSLKEIAKV LGAYEIVDYL ETLRVRVKYG IREELIPLMQ
660 670 680 690 700
LPLVGRRRAR ALYNSGFRSI EDISQARPEE LLKIEGIGVK TVEAIFKFLG
710 720
KNVKISEKPR KSTLDYFLKS
Length:720
Mass (Da):82,631
Last modified:November 1, 1998 - v1
Checksum:iBA17FCE53F615688
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016521 Genomic DNA. Translation: BAA32016.1.
AE009950 Genomic DNA. Translation: AAL80801.1.
PIRiT43854.
RefSeqiWP_011011799.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80801; AAL80801; PF0677.
GeneIDi1468523.
KEGGipfu:PF0677.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016521 Genomic DNA. Translation: BAA32016.1.
AE009950 Genomic DNA. Translation: AAL80801.1.
PIRiT43854.
RefSeqiWP_011011799.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZJ2X-ray2.40A1-720[»]
2ZJ5X-ray2.40A1-720[»]
2ZJ8X-ray2.00A1-720[»]
2ZJAX-ray2.70A1-720[»]
ProteinModelPortaliO73946.
SMRiO73946.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0677.

Proteomic databases

PRIDEiO73946.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80801; AAL80801; PF0677.
GeneIDi1468523.
KEGGipfu:PF0677.

Phylogenomic databases

eggNOGiarCOG00553. Archaea.
COG1204. LUCA.
HOGENOMiHOG000227028.
KOiK03726.
OMAiDEIHYLG.

Enzyme and pathway databases

BRENDAi3.6.4.12. 5243.

Miscellaneous databases

EvolutionaryTraceiO73946.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
HAMAPiMF_00442. Helicase_Hel308. 1 hit.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR022965. Helicase_Hel308.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00278. HhH1. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHELS_PYRFU
AccessioniPrimary (citable) accession number: O73946
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.