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Protein

Pyrrolidone-carboxylate peptidase

Gene

pcp

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Removes 5-oxoproline from various penultimate amino acid residues except L-proline.

Catalytic activityi

Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei79 – 791
Active sitei142 – 1421
Active sitei166 – 1661

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.19.3. 5243.

Protein family/group databases

MEROPSiC15.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrrolidone-carboxylate peptidase (EC:3.4.19.3)
Alternative name(s):
5-oxoprolyl-peptidase
Pyroglutamyl-peptidase I
Short name:
PGP-I
Short name:
Pyrase
Gene namesi
Name:pcp
Ordered Locus Names:PF1299
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 208208Pyrrolidone-carboxylate peptidasePRO_0000184755Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi188 – 188Interchain

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiO73944.

Interactioni

Subunit structurei

Homotetramer made of two disulfide-linked dimers.

Protein-protein interaction databases

STRINGi186497.PF1299.

Structurei

Secondary structure

1
208
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 87Combined sources
Helixi18 – 269Combined sources
Beta strandi34 – 418Combined sources
Helixi47 – 5913Combined sources
Beta strandi62 – 698Combined sources
Beta strandi75 – 795Combined sources
Beta strandi81 – 844Combined sources
Beta strandi99 – 1013Combined sources
Beta strandi109 – 1124Combined sources
Helixi117 – 12610Combined sources
Beta strandi131 – 1355Combined sources
Helixi141 – 15616Combined sources
Beta strandi160 – 1678Combined sources
Helixi171 – 1733Combined sources
Helixi175 – 1806Combined sources
Helixi189 – 20618Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IOFX-ray2.20A/B/C/D1-208[»]
1IOIX-ray2.70A/B/C/D1-208[»]
1X10X-ray2.00A/B/C/D1-208[»]
1X12X-ray2.00A/B/C/D1-208[»]
1Z8TX-ray2.50A/B/C/D1-208[»]
1Z8WX-ray2.00A/B/C/D1-208[»]
1Z8XX-ray2.00A/B/C/D1-208[»]
2DF5X-ray2.30A/B/C/D1-204[»]
2EO8X-ray2.30A/B/C/D1-208[»]
ProteinModelPortaliO73944.
SMRiO73944. Positions 1-208.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO73944.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C15 family.Curated

Phylogenomic databases

eggNOGiarCOG05850. Archaea.
COG2039. LUCA.
HOGENOMiHOG000242641.
KOiK01304.
OMAiVCNHIMY.

Family and domain databases

CDDicd00501. Peptidase_C15. 1 hit.
Gene3Di3.40.630.20. 1 hit.
HAMAPiMF_00417. Pyrrolid_peptidase. 1 hit.
InterProiIPR000816. Peptidase_C15.
IPR016125. Peptidase_C15-like.
IPR029762. PGP-I_bact-type.
IPR033694. PGPEP1_Cys_AS.
IPR033693. PGPEP1_Glu_AS.
[Graphical view]
PANTHERiPTHR23402. PTHR23402. 2 hits.
PfamiPF01470. Peptidase_C15. 1 hit.
[Graphical view]
PIRSFiPIRSF015592. Prld-crbxl_pptds. 1 hit.
PRINTSiPR00706. PYROGLUPTASE.
TIGRFAMsiTIGR00504. pyro_pdase. 1 hit.
PROSITEiPS01334. PYRASE_CYS. 1 hit.
PS01333. PYRASE_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O73944-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLVTGFEP FGGEKINPTE RIAKDLDGIK IGDAQVFGRV LPVVFGKAKE
60 70 80 90 100
VLEKTLEEIK PDIAIHVGLA PGRSAISIER IAVNAIDARI PDNEGKKIED
110 120 130 140 150
EPIVPGAPTA YFSTLPIKKI MKKLHERGIP AYISNSAGLY LCNYVMYLSL
160 170 180 190 200
HHSATKGYPK MSGFIHVPYI PEQIIDKIGK GQVPPSMCYE MELEAVKVAI

EVALEELL
Length:208
Mass (Da):22,822
Last modified:November 1, 1998 - v1
Checksum:i8977C19E55183BD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015291 Genomic DNA. Translation: BAA32989.1.
AE009950 Genomic DNA. Translation: AAL81423.1.
PIRiJE0314.
RefSeqiWP_011012443.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81423; AAL81423; PF1299.
GeneIDi1469172.
KEGGipfu:PF1299.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015291 Genomic DNA. Translation: BAA32989.1.
AE009950 Genomic DNA. Translation: AAL81423.1.
PIRiJE0314.
RefSeqiWP_011012443.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IOFX-ray2.20A/B/C/D1-208[»]
1IOIX-ray2.70A/B/C/D1-208[»]
1X10X-ray2.00A/B/C/D1-208[»]
1X12X-ray2.00A/B/C/D1-208[»]
1Z8TX-ray2.50A/B/C/D1-208[»]
1Z8WX-ray2.00A/B/C/D1-208[»]
1Z8XX-ray2.00A/B/C/D1-208[»]
2DF5X-ray2.30A/B/C/D1-204[»]
2EO8X-ray2.30A/B/C/D1-208[»]
ProteinModelPortaliO73944.
SMRiO73944. Positions 1-208.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1299.

Protein family/group databases

MEROPSiC15.001.

Proteomic databases

PRIDEiO73944.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81423; AAL81423; PF1299.
GeneIDi1469172.
KEGGipfu:PF1299.

Phylogenomic databases

eggNOGiarCOG05850. Archaea.
COG2039. LUCA.
HOGENOMiHOG000242641.
KOiK01304.
OMAiVCNHIMY.

Enzyme and pathway databases

BRENDAi3.4.19.3. 5243.

Miscellaneous databases

EvolutionaryTraceiO73944.

Family and domain databases

CDDicd00501. Peptidase_C15. 1 hit.
Gene3Di3.40.630.20. 1 hit.
HAMAPiMF_00417. Pyrrolid_peptidase. 1 hit.
InterProiIPR000816. Peptidase_C15.
IPR016125. Peptidase_C15-like.
IPR029762. PGP-I_bact-type.
IPR033694. PGPEP1_Cys_AS.
IPR033693. PGPEP1_Glu_AS.
[Graphical view]
PANTHERiPTHR23402. PTHR23402. 2 hits.
PfamiPF01470. Peptidase_C15. 1 hit.
[Graphical view]
PIRSFiPIRSF015592. Prld-crbxl_pptds. 1 hit.
PRINTSiPR00706. PYROGLUPTASE.
TIGRFAMsiTIGR00504. pyro_pdase. 1 hit.
PROSITEiPS01334. PYRASE_CYS. 1 hit.
PS01333. PYRASE_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCP_PYRFU
AccessioniPrimary (citable) accession number: O73944
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.