Reviewed, UniProtKB/Swiss-Prot O73930 (PHS_CHICK)

Names and origin

Protein names

Recommended name:
    Pterin-4-alpha-carbinolamine dehydratase
      Short name=PHS
    EC=4.2.1.96
Alternative name(s):
    4-alpha-hydroxy-tetrahydropterin dehydratase
    Phenylalanine hydroxylase-stimulating protein
    Pterin carbinolamine dehydratase
      Short name=PCD
    Dimerization cofactor of hepatocyte nuclear factor 1-alpha
      Short name=Dimerization cofactor of HNF1
      Short name=DCoH
      Short name=cDcoH

Gene names

Name:	PCBD1
Synonyms:	DCOH, PCBD

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	104 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2 By similarity. Regulates the dimerization of homeodomain protein HNF-1-alpha and enhances its transcriptional activity.
Catalytic activity	(6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin = (6R)-6-(L-erythro-1,2-dihydroxypropyl)-7,8-dihydro-6H-pterin + H2O.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Cytoplasmic and/or nuclear By similarity.
Tissue specificity	The major tissues expressing cDcoH are hypothalamus, kidney and liver. Ref.2
Sequence similarities	Belongs to the pterin-4-alpha-carbinolamine dehydratase family.

Ontologies

Keywords
Biological process	Tetrahydrobiopterin biosynthesis
Cellular component	Cytoplasm Nucleus
Molecular function	Lyase
PTM	Acetylation
Gene Ontology (GO)
Biological process	tetrahydrobiopterin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-hydroxytetrahydrobiopterin dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 104	103	Pterin-4-alpha-carbinolamine dehydratase		PRO_0000063055
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O73930-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 0786C7DF71A4EA99	FASTA	104	11,998
	10 20 30 40 50 60 MAGKAHRLNA EEREQLLPNL RAVGWNEVEG RDAIFKEFHF KDFNRAFGFM TRVALQAEKL 70 80 90 100 DHHPEWFNVY NKVHITLSTH ECTGLSERDI NLASFIEQVA ASLS

References

[1]	"Characterization of the chicken and rat DCoH gene domains using an improved ligation-mediated PCR method." Bossow S., Riepl S., Igo-Kemenes T. Biol. Chem. 379:1359-1365(1998) [PubMed: 9865610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"Differential expression of chicken dimerization cofactor of hepatocyte nuclear factor-1 (DcoH) and its novel counterpart, DcoHalpha." Kim H., You S., Foster L.K., Farris J., Choi Y.-J., Foster D.N. Biochem. J. 354:645-653(2001) [PubMed: 11237869] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ005158 Genomic DNA. Translation: CAA06395.1. AJ005157 mRNA. Translation: CAA06394.1.
IPI	IPI00583419.
RefSeq	NP_990236.1.
UniGene	Gga.427
3D structure databases
HSSP	HSSP built from PDB template 1F93 based on UniProtKB P80095.
SMR	O73930. Positions 2-104.
ModBase	Search...
Genome annotation databases
GeneID	395729.
KEGG	gga:395729.
Organism-specific databases
CTD	395729.
Phylogenomic databases
HOVERGEN	O73930.
Enzyme and pathway databases
BRENDA	4.2.1.96. 4.
Family and domain databases
InterPro	IPR001533. Trans/pterin_deHydtase. [Graphical view]
Gene3D	G3DSA:3.30.1360.20. Trans_pterinDh. 1 hit.
PANTHER	PTHR12599. Trans_pterinDh. 1 hit.
Pfam	PF01329. Pterin_4a. 1 hit. [Graphical view]
ProDom	PD007262. Trans_pterinDh. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

PHS_CHICK

Accession

Primary (citable) accession number: O73930

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	January 23, 2007
Last modified:	September 1, 2009
This is version 60 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)