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O73888

- HPGDS_CHICK

UniProt

O73888 - HPGDS_CHICK

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Protein

Hematopoietic prostaglandin D synthase

Gene

HPGDS

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Bifunctional enzyme which catalyzes both the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation, and the conjugation of glutathione with a wide range of aryl halides, organic isothiocyanates and alpha,beta-unsaturated carbonyls. Also exhibits low glutathione-peroxidase activity towards cumene hydroperoxide and t-butyl hydroperoxide.1 Publication

Catalytic activityi

(5Z,13E,15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E,15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate.1 Publication
RX + glutathione = HX + R-S-glutathione.1 Publication

Cofactori

glutathione1 PublicationNote: Glutathione is required for the prostaglandin D synthase activity.1 Publication

Kineticsi

    Vmax=128 µmol/min/mg enzyme with 1-bromo-2,4-dinitrobenzene as substrate1 Publication

    Vmax=97 µmol/min/mg enzyme with 1-chloro-2,4-dinitrobenzene as substrate1 Publication

    Vmax=410 µmol/min/mg enzyme with 1-fluoro-2,4-dinitrobenzene as substrate1 Publication

    Vmax=116 µmol/min/mg enzyme with 1-iodo-2,4-dinitrobenzene as substrate1 Publication

    Vmax=67 µmol/min/mg enzyme with 7-chloro-4-nitrobenz-2-oxa-1,3-diazole as substrate1 Publication

    Vmax=1.4 µmol/min/mg enzyme with 4-nitrobenzyl chloride as substrate1 Publication

    Vmax=0.05 µmol/min/mg enzyme with 1,2-dichloro-4-nitrobenzene as substrate1 Publication

    Vmax=0.04 µmol/min/mg enzyme with ethacrynic acid as substrate1 Publication

    Vmax=2.8 µmol/min/mg enzyme with 4-hydroxynon-2-enal as substrate1 Publication

    Vmax=0.06 µmol/min/mg enzyme with trans,trans-deca-2,4-dienal as substrate1 Publication

    Vmax=0.02 µmol/min/mg enzyme with trans-non-2-enal as substrate1 Publication

    Vmax=0.5 µmol/min/mg enzyme with cumene hydroperoxide as substrate1 Publication

    Vmax=0.06 µmol/min/mg enzyme with t-butyl hydroperoxide as substrate1 Publication

    Vmax=12.6 µmol/min/mg enzyme with allyl isothiocyanate as substrate1 Publication

    Vmax=17.6 µmol/min/mg enzyme with benzyl isothiocyanate as substrate1 Publication

    Vmax=0.02 µmol/min/mg enzyme with Delta5-androstene-3,17-dione as substrate1 Publication

    Vmax=0.11 µmol/min/mg enzyme with 4-nitrophenyl acetate as substrate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei8 – 81GlutathioneBy similarity
    Binding sitei14 – 141GlutathioneBy similarity
    Binding sitei39 – 391GlutathioneBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: UniProtKB
    2. glutathione transferase activity Source: UniProtKB-EC
    3. magnesium ion binding Source: UniProtKB
    4. prostaglandin-D synthase activity Source: UniProtKB

    GO - Biological processi

    1. prostaglandin biosynthetic process Source: UniProtKB-KW
    2. prostaglandin metabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Isomerase, Transferase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism

    Enzyme and pathway databases

    BRENDAi5.3.99.2. 1306.
    ReactomeiREACT_194363. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
    REACT_222911. Glutathione conjugation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hematopoietic prostaglandin D synthase (EC:5.3.99.2)
    Short name:
    H-PGDS
    Alternative name(s):
    GST class-sigma
    Glutathione S-transferase (EC:2.5.1.18)
    Glutathione-dependent PGD synthase
    Glutathione-requiring prostaglandin D synthase
    Prostaglandin-H2 D-isomerase
    Gene namesi
    Name:HPGDS
    Synonyms:GSTS, PGDS, PTGDS2
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 199198Hematopoietic prostaglandin D synthasePRO_0000185937Add
    BLAST

    Expressioni

    Tissue specificityi

    Highly expressed in liver, kidney, small intestine and colon, moderately in pancreas, bone marrow, lung and ovary, and expressed at low levels in spleen, thymus, heart and brain. Not detected in oviduct or skin (at protein level).1 Publication

    Gene expression databases

    ExpressionAtlasiO73888. baseline.

    Interactioni

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000016919.

    Structurei

    3D structure databases

    ProteinModelPortaliO73888.
    SMRiO73888. Positions 1-199.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 7978GST N-terminalAdd
    BLAST
    Domaini81 – 199119GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni63 – 642Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Sigma family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    HOGENOMiHOG000115733.
    HOVERGENiHBG105321.
    InParanoidiO73888.
    KOiK01830.
    OMAiWDVCSTT.
    OrthoDBiEOG78WKT1.
    PhylomeDBiO73888.
    TreeFamiTF105321.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O73888-1 [UniParc]FASTAAdd to Basket

    « Hide

            10         20         30         40         50
    MPNYKLTYFN LRGRAEICRY LFAYAGIKYE DHRLEGADWP KIKPTIPFGK
    60 70 80 90 100
    VPILEVDGVI IHQSLAIARY LARESGLAGQ TPVEQALADA IVDTIDDFMM
    110 120 130 140 150
    LFPWAEKNQD VKEKAFNDIL TNKAPELLKD LDTFLGDKKW FVGKSVTWAD
    160 170 180 190
    FYWDVCSTTL LSYKADLADK YPRLLALRDR VEALPAIAAW IQKRPKTAI
    Length:199
    Mass (Da):22,730
    Last modified:January 23, 2007 - v3
    Checksum:i335A884D93009069
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ006405 mRNA. Translation: CAA07005.1.
    RefSeqiNP_990342.1. NM_205011.1.
    UniGeneiGga.3137.

    Genome annotation databases

    GeneIDi395863.
    KEGGigga:395863.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ006405 mRNA. Translation: CAA07005.1 .
    RefSeqi NP_990342.1. NM_205011.1.
    UniGenei Gga.3137.

    3D structure databases

    ProteinModelPortali O73888.
    SMRi O73888. Positions 1-199.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000016919.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 395863.
    KEGGi gga:395863.

    Organism-specific databases

    CTDi 27306.

    Phylogenomic databases

    HOGENOMi HOG000115733.
    HOVERGENi HBG105321.
    InParanoidi O73888.
    KOi K01830.
    OMAi WDVCSTT.
    OrthoDBi EOG78WKT1.
    PhylomeDBi O73888.
    TreeFami TF105321.

    Enzyme and pathway databases

    BRENDAi 5.3.99.2. 1306.
    Reactomei REACT_194363. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
    REACT_222911. Glutathione conjugation.

    Miscellaneous databases

    NextBioi 20815930.
    PROi O73888.

    Gene expression databases

    ExpressionAtlasi O73888. baseline.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence, catalytic properties and expression of chicken glutathione-dependent prostaglandin D2 synthase, a novel class Sigma glutathione S-transferase."
      Thomson A.M., Meyer D.J., Hayes J.D.
      Biochem. J. 333:317-325(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
      Tissue: Spleen.

    Entry informationi

    Entry nameiHPGDS_CHICK
    AccessioniPrimary (citable) accession number: O73888
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 23, 2007
    Last modified: November 26, 2014
    This is version 100 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3