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O73888

- HPGDS_CHICK

UniProt

O73888 - HPGDS_CHICK

Protein

Hematopoietic prostaglandin D synthase

Gene

HPGDS

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Bifunctional enzyme which catalyzes both the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation, and the conjugation of glutathione with a wide range of aryl halides, organic isothiocyanates and alpha,beta-unsaturated carbonyls. Also exhibits low glutathione-peroxidase activity towards cumene hydroperoxide and t-butyl hydroperoxide.1 Publication

    Catalytic activityi

    (5Z,13E,15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E,15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate.1 Publication
    RX + glutathione = HX + R-S-glutathione.1 Publication

    Cofactori

    Glutathione. Required for the prostaglandin D synthase activity.1 Publication

    Kineticsi

      Vmax=128 µmol/min/mg enzyme with 1-bromo-2,4-dinitrobenzene as substrate1 Publication

      Vmax=97 µmol/min/mg enzyme with 1-chloro-2,4-dinitrobenzene as substrate1 Publication

      Vmax=410 µmol/min/mg enzyme with 1-fluoro-2,4-dinitrobenzene as substrate1 Publication

      Vmax=116 µmol/min/mg enzyme with 1-iodo-2,4-dinitrobenzene as substrate1 Publication

      Vmax=67 µmol/min/mg enzyme with 7-chloro-4-nitrobenz-2-oxa-1,3-diazole as substrate1 Publication

      Vmax=1.4 µmol/min/mg enzyme with 4-nitrobenzyl chloride as substrate1 Publication

      Vmax=0.05 µmol/min/mg enzyme with 1,2-dichloro-4-nitrobenzene as substrate1 Publication

      Vmax=0.04 µmol/min/mg enzyme with ethacrynic acid as substrate1 Publication

      Vmax=2.8 µmol/min/mg enzyme with 4-hydroxynon-2-enal as substrate1 Publication

      Vmax=0.06 µmol/min/mg enzyme with trans,trans-deca-2,4-dienal as substrate1 Publication

      Vmax=0.02 µmol/min/mg enzyme with trans-non-2-enal as substrate1 Publication

      Vmax=0.5 µmol/min/mg enzyme with cumene hydroperoxide as substrate1 Publication

      Vmax=0.06 µmol/min/mg enzyme with t-butyl hydroperoxide as substrate1 Publication

      Vmax=12.6 µmol/min/mg enzyme with allyl isothiocyanate as substrate1 Publication

      Vmax=17.6 µmol/min/mg enzyme with benzyl isothiocyanate as substrate1 Publication

      Vmax=0.02 µmol/min/mg enzyme with Delta5-androstene-3,17-dione as substrate1 Publication

      Vmax=0.11 µmol/min/mg enzyme with 4-nitrophenyl acetate as substrate1 Publication

      Sites

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Binding sitei8 – 81GlutathioneBy similarity
      Binding sitei14 – 141GlutathioneBy similarity
      Binding sitei39 – 391GlutathioneBy similarity

      GO - Molecular functioni

      1. calcium ion binding Source: UniProtKB
      2. glutathione transferase activity Source: UniProtKB-EC
      3. magnesium ion binding Source: UniProtKB
      4. prostaglandin-D synthase activity Source: UniProtKB

      GO - Biological processi

      1. prostaglandin biosynthetic process Source: UniProtKB-KW
      2. prostaglandin metabolic process Source: UniProtKB

      Keywords - Molecular functioni

      Isomerase, Transferase

      Keywords - Biological processi

      Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism

      Enzyme and pathway databases

      BRENDAi5.3.99.2. 1306.

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Hematopoietic prostaglandin D synthase (EC:5.3.99.2)
      Short name:
      H-PGDS
      Alternative name(s):
      GST class-sigma
      Glutathione S-transferase (EC:2.5.1.18)
      Glutathione-dependent PGD synthase
      Glutathione-requiring prostaglandin D synthase
      Prostaglandin-H2 D-isomerase
      Gene namesi
      Name:HPGDS
      Synonyms:GSTS, PGDS, PTGDS2
      OrganismiGallus gallus (Chicken)
      Taxonomic identifieri9031 [NCBI]
      Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
      ProteomesiUP000000539: Unplaced

      Subcellular locationi

      GO - Cellular componenti

      1. cytoplasm Source: UniProtKB-SubCell
      2. nucleus Source: Ensembl

      Keywords - Cellular componenti

      Cytoplasm

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Initiator methioninei1 – 11RemovedBy similarity
      Chaini2 – 199198Hematopoietic prostaglandin D synthasePRO_0000185937Add
      BLAST

      Expressioni

      Tissue specificityi

      Highly expressed in liver, kidney, small intestine and colon, moderately in pancreas, bone marrow, lung and ovary, and expressed at low levels in spleen, thymus, heart and brain. Not detected in oviduct or skin (at protein level).1 Publication

      Interactioni

      Protein-protein interaction databases

      STRINGi9031.ENSGALP00000016919.

      Structurei

      3D structure databases

      ProteinModelPortaliO73888.
      SMRiO73888. Positions 1-199.
      ModBaseiSearch...
      MobiDBiSearch...

      Family & Domainsi

      Domains and Repeats

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Domaini2 – 7978GST N-terminalAdd
      BLAST
      Domaini81 – 199119GST C-terminalAdd
      BLAST

      Region

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Regioni63 – 642Glutathione bindingBy similarity

      Sequence similaritiesi

      Belongs to the GST superfamily. Sigma family.Curated
      Contains 1 GST C-terminal domain.Curated
      Contains 1 GST N-terminal domain.Curated

      Phylogenomic databases

      HOGENOMiHOG000115733.
      HOVERGENiHBG105321.
      InParanoidiO73888.
      KOiK01830.
      OMAiWDVCSTT.
      OrthoDBiEOG78WKT1.
      PhylomeDBiO73888.
      TreeFamiTF105321.

      Family and domain databases

      Gene3Di1.20.1050.10. 1 hit.
      3.40.30.10. 1 hit.
      InterProiIPR010987. Glutathione-S-Trfase_C-like.
      IPR004045. Glutathione_S-Trfase_N.
      IPR004046. GST_C.
      IPR012336. Thioredoxin-like_fold.
      [Graphical view]
      PfamiPF00043. GST_C. 1 hit.
      PF02798. GST_N. 1 hit.
      [Graphical view]
      SUPFAMiSSF47616. SSF47616. 1 hit.
      SSF52833. SSF52833. 1 hit.
      PROSITEiPS50405. GST_CTER. 1 hit.
      PS50404. GST_NTER. 1 hit.
      [Graphical view]

      Sequencei

      Sequence statusi: Complete.

      Sequence processingi: The displayed sequence is further processed into a mature form.

      O73888-1 [UniParc]FASTAAdd to Basket

      « Hide

      MPNYKLTYFN LRGRAEICRY LFAYAGIKYE DHRLEGADWP KIKPTIPFGK    50
      VPILEVDGVI IHQSLAIARY LARESGLAGQ TPVEQALADA IVDTIDDFMM 100
      LFPWAEKNQD VKEKAFNDIL TNKAPELLKD LDTFLGDKKW FVGKSVTWAD 150
      FYWDVCSTTL LSYKADLADK YPRLLALRDR VEALPAIAAW IQKRPKTAI 199
      Length:199
      Mass (Da):22,730
      Last modified:January 23, 2007 - v3
      Checksum:i335A884D93009069
      GO

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      AJ006405 mRNA. Translation: CAA07005.1.
      RefSeqiNP_990342.1. NM_205011.1.
      UniGeneiGga.3137.

      Genome annotation databases

      GeneIDi395863.
      KEGGigga:395863.

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      AJ006405 mRNA. Translation: CAA07005.1 .
      RefSeqi NP_990342.1. NM_205011.1.
      UniGenei Gga.3137.

      3D structure databases

      ProteinModelPortali O73888.
      SMRi O73888. Positions 1-199.
      ModBasei Search...
      MobiDBi Search...

      Protein-protein interaction databases

      STRINGi 9031.ENSGALP00000016919.

      Protocols and materials databases

      Structural Biology Knowledgebase Search...

      Genome annotation databases

      GeneIDi 395863.
      KEGGi gga:395863.

      Organism-specific databases

      CTDi 27306.

      Phylogenomic databases

      HOGENOMi HOG000115733.
      HOVERGENi HBG105321.
      InParanoidi O73888.
      KOi K01830.
      OMAi WDVCSTT.
      OrthoDBi EOG78WKT1.
      PhylomeDBi O73888.
      TreeFami TF105321.

      Enzyme and pathway databases

      BRENDAi 5.3.99.2. 1306.

      Miscellaneous databases

      NextBioi 20815930.
      PROi O73888.

      Family and domain databases

      Gene3Di 1.20.1050.10. 1 hit.
      3.40.30.10. 1 hit.
      InterProi IPR010987. Glutathione-S-Trfase_C-like.
      IPR004045. Glutathione_S-Trfase_N.
      IPR004046. GST_C.
      IPR012336. Thioredoxin-like_fold.
      [Graphical view ]
      Pfami PF00043. GST_C. 1 hit.
      PF02798. GST_N. 1 hit.
      [Graphical view ]
      SUPFAMi SSF47616. SSF47616. 1 hit.
      SSF52833. SSF52833. 1 hit.
      PROSITEi PS50405. GST_CTER. 1 hit.
      PS50404. GST_NTER. 1 hit.
      [Graphical view ]
      ProtoNeti Search...

      Publicationsi

      1. "Sequence, catalytic properties and expression of chicken glutathione-dependent prostaglandin D2 synthase, a novel class Sigma glutathione S-transferase."
        Thomson A.M., Meyer D.J., Hayes J.D.
        Biochem. J. 333:317-325(1998) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
        Tissue: Spleen.

      Entry informationi

      Entry nameiHPGDS_CHICK
      AccessioniPrimary (citable) accession number: O73888
      Entry historyi
      Integrated into UniProtKB/Swiss-Prot: May 30, 2000
      Last sequence update: January 23, 2007
      Last modified: October 1, 2014
      This is version 98 of the entry and version 3 of the sequence. [Complete history]
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programChordata Protein Annotation Program

      Miscellaneousi

      Keywords - Technical termi

      Complete proteome, Reference proteome

      Documents

      1. SIMILARITY comments
        Index of protein domains and families

      External Data

      Dasty 3