Reviewed, UniProtKB/Swiss-Prot O73884 (PHOP1_CHICK)

Names and origin

Protein names

Recommended name:
    Phosphoethanolamine/phosphocholine phosphatase
    EC=3.1.3.75
Alternative name(s):
    3X11A

Gene names

Name:

PHOSPHO1

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). May be involved in the generation of inorganic phosphate for bone mineralization. Ref.2
Catalytic activity	O-phosphoethanolamine + H2O = ethanolamine + phosphate. Phosphocholine + H2O = choline + phosphate.
Cofactor	Magnesium By similarity.
Tissue specificity	Expressed at sites of mineralization in bone and cartilage. Highly expressed in hypertrophic chondrocytes compared to non-chondrogenic tissues. Expressed in chondrocytes but not in heart, liver, lung, kidney, spleen, muscle, adipose tissues not duodenum. In diaphyseal cortical bone, it is expressed in the osteoid layer of the periosteum, forming surfaces of growing osteons, and newly formed osteocytes, whereas it is not expressed in the endosteum and closed osteons. In growth plate cartilage, it is limited to the early hypertrophic chondrocytes and the ossification groove of Ranvier. Highly expressed on the mineralization surfaces of the cartilage remnants and trabecular bone within the primary spongiosa. Expressed in 17-day-old embryonic calvaria, the osteoid present on the intramembranous and periosteal bone surfaces but not in soft tissues examined. Ref.2 Ref.1
Induction	Up-regulated 5-fold during chondrocyte terminal differentiation. Ref.1
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoethanolamine/phosphocholine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Phosphoethanolamine/phosphocholine phosphatase		PRO_0000068831
Sites
	Active site	32	1	Nucleophile By similarity
	Active site	34	1	Proton donor By similarity
	Metal binding	32	1	Magnesium By similarity
	Metal binding	34	1	Magnesium By similarity
	Metal binding	203	1	Magnesium By similarity
	Binding site	43	1	Substrate By similarity
	Binding site	123	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O73884-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 68852F21D9EE0024	FASTA	268	30,443
	10 20 30 40 50 60 MKRCCEGVGL PCLFKGVGMA SSRPPKYLLV FDFDGTIINE SSDDSIVRAA PGQALPEHIR 70 80 90 100 110 120 QSFREGFYNE YMQRVLAYMG DQGVKMGDFK AVYENIPLSP GMPDLFQFLS KNHELFEIIL 130 140 150 160 170 180 ISDANMFGIE CKLRAAGFYS LFRKIFSNPS SFDKRGYFTL GPYHSHKCLD CPANTCKRKI 190 200 210 220 230 240 LTEYLAERAQ EEVEFERVFY VGDGANDFCP SVTLTSADVA FPRKGYPMHQ MTQEMEKKQP 250 260 GTFQATVVPW ESATEVARYL QELLKKKC

References

[1]	"Identification and cloning of a novel phosphatase expressed at high levels in differentiating growth plate chondrocytes." Houston B., Seawright E., Jefferies D., Hoogland E., Lester D., Whitehead C., Farquharson C. Biochim. Biophys. Acta 1448:500-506(1999) [PubMed: 9990301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
[2]	"PHOSPHO1 -- a novel phosphatase specifically expressed at sites of mineralisation in bone and cartilage." Houston B., Stewart A.J., Farquharson C. Bone 34:629-637(2004) [PubMed: 15050893] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ006529 mRNA. Translation: CAA07090.1.
IPI	IPI00577667.
RefSeq	NP_990176.1.
UniGene	Gga.2334
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSGALG00000001281. Gallus gallus. [Contig view]
GeneID	395650.
KEGG	gga:395650.
Phylogenomic databases
HOVERGEN	O73884.
Enzyme and pathway databases
BRENDA	3.1.3.75. 4.
Family and domain databases
InterPro	IPR006383. HAD-SF_hydro_IB_PSP-like. IPR006384. PyrdxlP_Pase-rel. IPR016965. PyrdxlP_Pase_PHOSPHO2. [Graphical view]
Pfam	PF06888. Put_Phosphatase. 1 hit. [Graphical view]
PIRSF	PIRSF031051. PyrdxlP_Pase_PHOSPHO2. 1 hit.
TIGRFAMs	TIGR01489. DKMTPPase-SF. 1 hit. TIGR01488. HAD-SF-IB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PHOP1_CHICK

Accession

Primary (citable) accession number: O73884

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	August 1, 1998
Last modified:	March 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)