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Protein

Ephrin-B2a

Gene

efnb2a

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with ephb4 may play a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • angioblast cell migration involved in selective angioblast sprouting Source: ZFIN
  • anterior/posterior pattern specification Source: ZFIN
  • axon guidance Source: GO_Central
  • axon target recognition Source: ZFIN
  • blood vessel development Source: ZFIN
  • cell adhesion Source: UniProtKB
  • cell migration involved in sprouting angiogenesis Source: UniProtKB
  • ephrin receptor signaling pathway Source: UniProtKB
  • optic vesicle morphogenesis Source: ZFIN
  • regulation of chemotaxis Source: UniProtKB
  • rhombomere boundary formation Source: ZFIN
  • somitogenesis Source: ZFIN
  • synaptic target recognition Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Angiogenesis, Differentiation, Neurogenesis

Enzyme and pathway databases

ReactomeiR-DRE-2682334. EPH-Ephrin signaling.
R-DRE-3928662. EPHB-mediated forward signaling.
R-DRE-3928664. Ephrin signaling.
R-DRE-3928665. EPH-ephrin mediated repulsion of cells.

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin-B2a
Gene namesi
Name:efnb2a
Synonyms:efnb2
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 9

Organism-specific databases

ZFINiZDB-GENE-990415-67. efnb2a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 225201ExtracellularSequence analysisAdd
BLAST
Transmembranei226 – 24621HelicalSequence analysisAdd
BLAST
Topological domaini247 – 33286CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 332308Ephrin-B2aPRO_0000008394Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi20 – 201N-linked (GlcNAc...)Sequence analysis
Glycosylationi33 – 331N-linked (GlcNAc...)Sequence analysis
Disulfide bondi59 ↔ 98PROSITE-ProRule annotation
Disulfide bondi86 ↔ 150PROSITE-ProRule annotation
Glycosylationi136 – 1361N-linked (GlcNAc...)Sequence analysis
Glycosylationi211 – 2111N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO73874.

Expressioni

Gene expression databases

BgeeiO73874.

Interactioni

Subunit structurei

Binds to the receptor tyrosine kinase ephb4.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000010432.

Structurei

3D structure databases

ProteinModelPortaliO73874.
SMRiO73874. Positions 22-163.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 161137Ephrin RBDPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi330 – 3323PDZ-bindingSequence analysis

Sequence similaritiesi

Belongs to the ephrin family.PROSITE-ProRule annotation
Contains 1 ephrin RBD (ephrin receptor-binding) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3858. Eukaryota.
ENOG4111FMJ. LUCA.
GeneTreeiENSGT00390000005839.
HOGENOMiHOG000220931.
HOVERGENiHBG051448.
InParanoidiO73874.
KOiK05463.
OMAiGSTRHND.
OrthoDBiEOG7288S5.
PhylomeDBiO73874.

Family and domain databases

Gene3Di2.60.40.420. 1 hit.
InterProiIPR008972. Cupredoxin.
IPR031328. Ephrin.
IPR019765. Ephrin_CS.
IPR001799. Ephrin_RBD.
[Graphical view]
PANTHERiPTHR11304. PTHR11304. 1 hit.
PfamiPF00812. Ephrin. 1 hit.
[Graphical view]
PRINTSiPR01347. EPHRIN.
ProDomiPD002533. Ephrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF49503. SSF49503. 1 hit.
PROSITEiPS01299. EPHRIN_RBD_1. 1 hit.
PS51551. EPHRIN_RBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O73874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDSLWRYYF GVLVIACKVN LSRALILDSI YWNTTNTKFV PGQGLVLYPQ
60 70 80 90 100
IGDKMDIVCP RVEGGSMEGV EYYKLYMVPL EQLKSCQVTK ADTPLLNCVK
110 120 130 140 150
PDQDVKFTLK FQEFSPNLWG LEFFRGKDYY IISTSNGTME GLDNQEGGVC
160 170 180 190 200
KTKSMKIIMK VGQNPSDPIS PKDYPTSYPP KHPDLGGKDS KSNEVLKPDA
210 220 230 240 250
SPHGEDKGDG NKSSSVIGSE VALFACIASA SVIVIIIIIM LVFLLLKYRR
260 270 280 290 300
RHRKHSPQHA TTLSLSTLAT PKRGGSGGNN NGSEPSDIII PLRTADSVFC
310 320 330
PHYEKVSGDY GHPVYIVQEM PPQSPANIYY KV
Length:332
Mass (Da):36,724
Last modified:August 1, 1998 - v1
Checksum:i189ED82372C71C8B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ004863 mRNA. Translation: CAA06168.1.
AF375225 mRNA. Translation: AAK64275.1.
RefSeqiNP_571098.1. NM_131023.1.
UniGeneiDr.75819.

Genome annotation databases

EnsembliENSDART00000026011; ENSDARP00000010432; ENSDARG00000020164.
GeneIDi30219.
KEGGidre:30219.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ004863 mRNA. Translation: CAA06168.1.
AF375225 mRNA. Translation: AAK64275.1.
RefSeqiNP_571098.1. NM_131023.1.
UniGeneiDr.75819.

3D structure databases

ProteinModelPortaliO73874.
SMRiO73874. Positions 22-163.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000010432.

Proteomic databases

PaxDbiO73874.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000026011; ENSDARP00000010432; ENSDARG00000020164.
GeneIDi30219.
KEGGidre:30219.

Organism-specific databases

CTDi30219.
ZFINiZDB-GENE-990415-67. efnb2a.

Phylogenomic databases

eggNOGiKOG3858. Eukaryota.
ENOG4111FMJ. LUCA.
GeneTreeiENSGT00390000005839.
HOGENOMiHOG000220931.
HOVERGENiHBG051448.
InParanoidiO73874.
KOiK05463.
OMAiGSTRHND.
OrthoDBiEOG7288S5.
PhylomeDBiO73874.

Enzyme and pathway databases

ReactomeiR-DRE-2682334. EPH-Ephrin signaling.
R-DRE-3928662. EPHB-mediated forward signaling.
R-DRE-3928664. Ephrin signaling.
R-DRE-3928665. EPH-ephrin mediated repulsion of cells.

Miscellaneous databases

PROiO73874.

Gene expression databases

BgeeiO73874.

Family and domain databases

Gene3Di2.60.40.420. 1 hit.
InterProiIPR008972. Cupredoxin.
IPR031328. Ephrin.
IPR019765. Ephrin_CS.
IPR001799. Ephrin_RBD.
[Graphical view]
PANTHERiPTHR11304. PTHR11304. 1 hit.
PfamiPF00812. Ephrin. 1 hit.
[Graphical view]
PRINTSiPR01347. EPHRIN.
ProDomiPD002533. Ephrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF49503. SSF49503. 1 hit.
PROSITEiPS01299. EPHRIN_RBD_1. 1 hit.
PS51551. EPHRIN_RBD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Eph signaling is required for segmentation and differentiation of the somites."
    Durbin L., Brennan C., Shiomi K., Cooke J., Barrios A., Shanmugalingam S., Guthrie B., Lindberg R., Holder N.
    Genes Dev. 12:3096-3109(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Morphogenesis of prechordal plate and notochord requires intact eph/ephrin b signaling."
    Chan J., Mably J.D., Serluca F.C., Chen J.N., Goldstein N.B., Thomas M.C., Cleary J.A., Brennan C., Fishman M.C., Roberts T.M.
    Dev. Biol. 234:470-482(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiEFNB2_DANRE
AccessioniPrimary (citable) accession number: O73874
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: July 6, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.