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O73853 (CP17A_ICTPU) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase

EC=1.14.99.9
EC=4.1.2.30
Alternative name(s):
17-alpha-hydroxyprogesterone aldolase
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name=Cytochrome P450c17
Gene names
Name:cyp17a1
Synonyms:cyp17
OrganismIctalurus punctatus (Channel catfish) (Silurus punctatus)
Taxonomic identifier7998 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiSiluriformesIctaluridaeIctalurus

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.

Catalytic activity

A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

Cofactor

Heme group By similarity.

Pathway

Lipid metabolism; steroid biosynthesis.

Subcellular location

Membrane Potential.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Biological processSteroidogenesis
   Cellular componentMembrane
   LigandHeme
Iron
Metal-binding
   Molecular functionLyase
Monooxygenase
Oxidoreductase
Gene Ontology (GO)
   Biological_processsteroid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentmembrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function17-alpha-hydroxyprogesterone aldolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

heme binding

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: InterPro

steroid 17-alpha-monooxygenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 514514Steroid 17-alpha-hydroxylase/17,20 lyase
PRO_0000051945

Sites

Metal binding4451Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
O73853 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 7CDF5CC0F6982053

FASTA51458,134
        10         20         30         40         50         60 
MGWLICFCVF AAIIIVALYL RKIHGFLVDD RAPPNLPSLP IIGSLLSINS NSPPHIFFQQ 

        70         80         90        100        110        120 
LQKKYGDIYS LDMGSNRVII VNNHHHAKEV LLRKGKIFAG RPRTVTTDIL TRDGKDIAFG 

       130        140        150        160        170        180 
DYSATWKFHR KIVHGALCMF GEGTASIEKI ICREASSMCE ILTNLQSSAA DLAPELTRAV 

       190        200        210        220        230        240 
TNVVCTLCFS SSYKRGDPEF EAMLKYSQGI VDTVAKDSLV DIFPWLQLFP NEDLRILRRC 

       250        260        270        280        290        300 
VSIRDKLLQK KYEEHKADFS DNIQRDLFDA LLRAKNSSEN NNTSTQDVGL TDDHLLMTVG 

       310        320        330        340        350        360 
DIFGAGVETT TTVLKWSILY LIHHPQVQRK IQEELDAKIG RDRHPQVNDR GNLPYLEATI 

       370        380        390        400        410        420 
REVLRIRPVS PLLIPHVALS DANIGEYTVQ KGTRVIVNLW SLHHDEKEWK NPELFNPERF 

       430        440        450        460        470        480 
LNEEGNSLCC PSLSYLPFGA GVRVCLGEAL AKLELFLFLS WILQRFTLEV PGGQPLPELQ 

       490        500        510 
GKFRVVLQPQ KYKVIARLRT GWEKSLQSQE SESG 

« Hide

References

[1]"Isolation and heterologous expression of the cDNA encoding the cytochrome P450 17-hydroxylase from the channel catfish (Ictalurus punctatus)."
Trant J.M., Berard C., Byrne B.J., Wunder J.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Ovary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF063837 mRNA. Translation: AAC16551.1.
RefSeqNP_001187242.1. NM_001200313.1.
UniGeneIpu.32457.

3D structure databases

ProteinModelPortalO73853.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100313515.

Organism-specific databases

CTD100313515.

Phylogenomic databases

HOVERGENHBG106944.

Enzyme and pathway databases

UniPathwayUPA00062.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP17A_ICTPU
AccessionPrimary (citable) accession number: O73853
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: December 11, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways