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Protein

Steroid 17-alpha-hydroxylase/17,20 lyase

Gene

cyp17a1

Organism
Ictalurus punctatus (Channel catfish) (Silurus punctatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.

Catalytic activityi

A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.
17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

Cofactori

hemeBy similarity

Pathway:isteroid biosynthesis

This protein is involved in the pathway steroid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway steroid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi445 – 4451Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase, Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00062.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase (EC:1.14.99.9, EC:4.1.2.30)
Alternative name(s):
17-alpha-hydroxyprogesterone aldolase
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name:
Cytochrome P450c17
Gene namesi
Name:cyp17a1
Synonyms:cyp17
OrganismiIctalurus punctatus (Channel catfish) (Silurus punctatus)
Taxonomic identifieri7998 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiSiluriformesIctaluridaeIctalurus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 514514Steroid 17-alpha-hydroxylase/17,20 lyasePRO_0000051945Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO73853.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

HOVERGENiHBG106944.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O73853-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGWLICFCVF AAIIIVALYL RKIHGFLVDD RAPPNLPSLP IIGSLLSINS
60 70 80 90 100
NSPPHIFFQQ LQKKYGDIYS LDMGSNRVII VNNHHHAKEV LLRKGKIFAG
110 120 130 140 150
RPRTVTTDIL TRDGKDIAFG DYSATWKFHR KIVHGALCMF GEGTASIEKI
160 170 180 190 200
ICREASSMCE ILTNLQSSAA DLAPELTRAV TNVVCTLCFS SSYKRGDPEF
210 220 230 240 250
EAMLKYSQGI VDTVAKDSLV DIFPWLQLFP NEDLRILRRC VSIRDKLLQK
260 270 280 290 300
KYEEHKADFS DNIQRDLFDA LLRAKNSSEN NNTSTQDVGL TDDHLLMTVG
310 320 330 340 350
DIFGAGVETT TTVLKWSILY LIHHPQVQRK IQEELDAKIG RDRHPQVNDR
360 370 380 390 400
GNLPYLEATI REVLRIRPVS PLLIPHVALS DANIGEYTVQ KGTRVIVNLW
410 420 430 440 450
SLHHDEKEWK NPELFNPERF LNEEGNSLCC PSLSYLPFGA GVRVCLGEAL
460 470 480 490 500
AKLELFLFLS WILQRFTLEV PGGQPLPELQ GKFRVVLQPQ KYKVIARLRT
510
GWEKSLQSQE SESG
Length:514
Mass (Da):58,134
Last modified:August 1, 1998 - v1
Checksum:i7CDF5CC0F6982053
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063837 mRNA. Translation: AAC16551.1.
RefSeqiNP_001187242.1. NM_001200313.1.
UniGeneiIpu.32457.

Genome annotation databases

GeneIDi100313515.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063837 mRNA. Translation: AAC16551.1.
RefSeqiNP_001187242.1. NM_001200313.1.
UniGeneiIpu.32457.

3D structure databases

ProteinModelPortaliO73853.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100313515.

Organism-specific databases

CTDi100313515.

Phylogenomic databases

HOVERGENiHBG106944.

Enzyme and pathway databases

UniPathwayiUPA00062.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and heterologous expression of the cDNA encoding the cytochrome P450 17-hydroxylase from the channel catfish (Ictalurus punctatus)."
    Trant J.M., Berard C., Byrne B.J., Wunder J.
    Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiCP17A_ICTPU
AccessioniPrimary (citable) accession number: O73853
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: January 7, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.