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Protein

Heterochromatin-associated protein MENT

Gene

SERPINB10

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein that promotes DNA condensation into transcriptionally inactive heterochromatin in terminally differentiated avian blood cells. Promotes tight packing of nucleosomes into spherical clusters by binding to linker DNA and subsequent oligomerization. Acts as a cysteine protease inhibitor towards CTSL (cathepsin L1) and CTSV (cathepsin L2), but does not inhibit serine proteases.5 Publications

GO - Molecular functioni

  • chromatin DNA binding Source: AgBase
  • serine-type endopeptidase inhibitor activity Source: GO_Central

GO - Biological processi

  • chromatin modification Source: UniProtKB-KW
  • chromosome condensation Source: AgBase
  • nucleate erythrocyte maturation Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Protease inhibitor

Keywords - Biological processi

DNA condensation

Keywords - Ligandi

DNA-binding

Protein family/group databases

MEROPSiI04.033.

Names & Taxonomyi

Protein namesi
Recommended name:
Heterochromatin-associated protein MENT
Alternative name(s):
Myeloid and erythroid nuclear termination stage-specific protein
Serpin B10
Gene namesi
Name:SERPINB10
Synonyms:MENT
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • extracellular space Source: GO_Central
  • nuclear chromatin Source: AgBase
  • nucleoplasm Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi73 – 797RPSRGRP → KGAKAKG: Reduced nuclear localization. 1 Publication
Mutagenesisi78 – 781R → Q: Reduced interaction with DNA. 1 Publication
Mutagenesisi99 – 991K → Q: Reduced interaction with DNA; when associated with Q-107 and Q-109. 1 Publication
Mutagenesisi107 – 1071K → Q: Reduced interaction with DNA; when associated with Q-99 and Q-109. 1 Publication
Mutagenesisi109 – 1091R → Q: Reduced interaction with DNA; when associated with Q-99 and Q-107. 1 Publication
Mutagenesisi138 – 1381K → Q: Reduced interaction with DNA. 1 Publication
Mutagenesisi214 – 2141R → A: Reduced interaction with DNA. 1 Publication
Mutagenesisi332 – 3321R → A: Reduced interaction with DNA. 1 Publication
Mutagenesisi338 – 3381K → A: Reduced interaction with DNA. 1 Publication
Mutagenesisi358 – 3581T → R: Strongly reduced inhibition of cysteine proteases. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Heterochromatin-associated protein MENTPRO_0000355551Add
BLAST

Proteomic databases

PaxDbiO73790.

Expressioni

Tissue specificityi

Detected in all major blood cell types. Highly expressed in granulocytes (at protein level).1 Publication

Gene expression databases

BgeeiENSGALG00000019553.

Interactioni

Subunit structurei

Homodimer and homooligomer. Interaction with DNA promotes oligomerization.2 Publications

Protein-protein interaction databases

STRINGi9031.ENSGALP00000020960.

Structurei

Secondary structure

410
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1 – 2222Combined sources
Beta strandi24 – 263Combined sources
Beta strandi28 – 303Combined sources
Helixi32 – 4312Combined sources
Helixi48 – 5710Combined sources
Helixi92 – 10514Combined sources
Beta strandi110 – 12314Combined sources
Helixi130 – 14011Combined sources
Beta strandi143 – 1475Combined sources
Turni149 – 1513Combined sources
Helixi153 – 16715Combined sources
Turni168 – 1703Combined sources
Beta strandi171 – 1733Combined sources
Turni178 – 1803Combined sources
Beta strandi187 – 20115Combined sources
Helixi205 – 2073Combined sources
Beta strandi209 – 2168Combined sources
Beta strandi219 – 23719Combined sources
Turni238 – 2414Combined sources
Beta strandi242 – 2498Combined sources
Helixi250 – 2523Combined sources
Beta strandi253 – 26311Combined sources
Beta strandi266 – 2705Combined sources
Helixi272 – 2765Combined sources
Helixi280 – 2878Combined sources
Beta strandi293 – 30210Combined sources
Beta strandi304 – 3118Combined sources
Helixi313 – 3197Combined sources
Helixi323 – 3253Combined sources
Turni332 – 3343Combined sources
Beta strandi336 – 3383Combined sources
Beta strandi341 – 35313Combined sources
Beta strandi355 – 36915Combined sources
Beta strandi379 – 3824Combined sources
Beta strandi387 – 3937Combined sources
Turni394 – 3974Combined sources
Beta strandi398 – 4058Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DUTX-ray3.00A/B/C/D1-410[»]
2H4PX-ray1.70A1-369[»]
B377-410[»]
2H4QX-ray2.10A1-369[»]
B377-410[»]
2H4RX-ray2.70A1-410[»]
ProteinModelPortaliO73790.
SMRiO73790. Positions 1-410.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO73790.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni61 – 699M-loop required for interaction with nucleosome linker DNA
Regioni356 – 37217Required for oligomerization and DNA condensationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi80 – 845Nuclear localization signalBy similarity

Sequence similaritiesi

Belongs to the serpin family. Ov-serpin subfamily.Curated

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
GeneTreeiENSGT00760000118789.
HOGENOMiHOG000238519.
HOVERGENiHBG005957.
InParanoidiO73790.
KOiK13963.
OMAiRMDPEHE.
OrthoDBiEOG091G0ION.
PhylomeDBiO73790.
TreeFamiTF352619.

Family and domain databases

InterProiIPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 2 hits.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 2 hits.

Sequencei

Sequence statusi: Complete.

O73790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQVSASIGN FTVDLFNKLN ETNRDKNIFF SPWSISSALA LTYLAAKGST
60 70 80 90 100
AREMAEVLHF TEAVRAESSS VARPSRGRPK RRRMDPEHEQ AENIHSGFKE
110 120 130 140 150
LLTAFNKPRN NYSLRSANRI YVEKTYALLP TYLQLSKKYY KAEPQKVNFK
160 170 180 190 200
TAPEQSRKEI NTWVEKQTES KIKNLLSSDD VKATTRLILV NAIYFKAEWE
210 220 230 240 250
VKFQAEKTSI QPFRLSKNKS KPVKMMYMRD TFPVLIMEKM NFKMIELPYV
260 270 280 290 300
KRELSMFILL PDDIKDGTTG LEQLERELTY ERLSEWADSK MMTETLVDLH
310 320 330 340 350
LPKFSLEDRI DLRDTLRNMG MTTAFTTNAD FRGMTDKKDL AISKVIHQSF
360 370 380 390 400
VAVDEKGTEA AAATAVIISF TTSVINHVLK FKVDHPFHFF IRHNKSKTIL
410
FFGRFCCPVE
Length:410
Mass (Da):47,384
Last modified:August 1, 1998 - v1
Checksum:i24FC06D9C4F4F0BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053401 mRNA. Translation: AAC15710.1.
RefSeqiNP_990228.1. NM_204897.1.
XP_015137649.1. XM_015282163.1.
XP_015137651.1. XM_015282165.1.
UniGeneiGga.415.

Genome annotation databases

EnsembliENSGALT00000020990; ENSGALP00000020960; ENSGALG00000019553.
GeneIDi395715.
KEGGigga:395715.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053401 mRNA. Translation: AAC15710.1.
RefSeqiNP_990228.1. NM_204897.1.
XP_015137649.1. XM_015282163.1.
XP_015137651.1. XM_015282165.1.
UniGeneiGga.415.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DUTX-ray3.00A/B/C/D1-410[»]
2H4PX-ray1.70A1-369[»]
B377-410[»]
2H4QX-ray2.10A1-369[»]
B377-410[»]
2H4RX-ray2.70A1-410[»]
ProteinModelPortaliO73790.
SMRiO73790. Positions 1-410.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000020960.

Protein family/group databases

MEROPSiI04.033.

Proteomic databases

PaxDbiO73790.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000020990; ENSGALP00000020960; ENSGALG00000019553.
GeneIDi395715.
KEGGigga:395715.

Organism-specific databases

CTDi5273.

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
GeneTreeiENSGT00760000118789.
HOGENOMiHOG000238519.
HOVERGENiHBG005957.
InParanoidiO73790.
KOiK13963.
OMAiRMDPEHE.
OrthoDBiEOG091G0ION.
PhylomeDBiO73790.
TreeFamiTF352619.

Miscellaneous databases

EvolutionaryTraceiO73790.
PROiO73790.

Gene expression databases

BgeeiENSGALG00000019553.

Family and domain databases

InterProiIPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 2 hits.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiSPB10_CHICK
AccessioniPrimary (citable) accession number: O73790
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.