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Protein

Heterochromatin-associated protein MENT

Gene

SERPINB10

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein that promotes DNA condensation into transcriptionally inactive heterochromatin in terminally differentiated avian blood cells. Promotes tight packing of nucleosomes into spherical clusters by binding to linker DNA and subsequent oligomerization. Acts as a cysteine protease inhibitor towards CTSL (cathepsin L1) and CTSV (cathepsin L2), but does not inhibit serine proteases.5 Publications

GO - Molecular functioni

  • chromatin DNA binding Source: AgBase
  • serine-type endopeptidase inhibitor activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Protease inhibitor

Keywords - Biological processi

DNA condensation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-GGA-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiI04.033.

Names & Taxonomyi

Protein namesi
Recommended name:
Heterochromatin-associated protein MENT
Alternative name(s):
Myeloid and erythroid nuclear termination stage-specific protein
Serpin B10
Gene namesi
Name:SERPINB10
Synonyms:MENT
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • extracellular space Source: GO_Central
  • nuclear chromatin Source: AgBase
  • nucleoplasm Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73 – 79RPSRGRP → KGAKAKG: Reduced nuclear localization. 1 Publication7
Mutagenesisi78R → Q: Reduced interaction with DNA. 1 Publication1
Mutagenesisi99K → Q: Reduced interaction with DNA; when associated with Q-107 and Q-109. 1 Publication1
Mutagenesisi107K → Q: Reduced interaction with DNA; when associated with Q-99 and Q-109. 1 Publication1
Mutagenesisi109R → Q: Reduced interaction with DNA; when associated with Q-99 and Q-107. 1 Publication1
Mutagenesisi138K → Q: Reduced interaction with DNA. 1 Publication1
Mutagenesisi214R → A: Reduced interaction with DNA. 1 Publication1
Mutagenesisi332R → A: Reduced interaction with DNA. 1 Publication1
Mutagenesisi338K → A: Reduced interaction with DNA. 1 Publication1
Mutagenesisi358T → R: Strongly reduced inhibition of cysteine proteases. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003555511 – 410Heterochromatin-associated protein MENTAdd BLAST410

Proteomic databases

PaxDbiO73790.

Expressioni

Tissue specificityi

Detected in all major blood cell types. Highly expressed in granulocytes (at protein level).1 Publication

Gene expression databases

BgeeiENSGALG00000019553.

Interactioni

Subunit structurei

Homodimer and homooligomer. Interaction with DNA promotes oligomerization.2 Publications

Protein-protein interaction databases

STRINGi9031.ENSGALP00000020960.

Structurei

Secondary structure

1410
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 22Combined sources22
Beta strandi24 – 26Combined sources3
Beta strandi28 – 30Combined sources3
Helixi32 – 43Combined sources12
Helixi48 – 57Combined sources10
Helixi92 – 105Combined sources14
Beta strandi110 – 123Combined sources14
Helixi130 – 140Combined sources11
Beta strandi143 – 147Combined sources5
Turni149 – 151Combined sources3
Helixi153 – 167Combined sources15
Turni168 – 170Combined sources3
Beta strandi171 – 173Combined sources3
Turni178 – 180Combined sources3
Beta strandi187 – 201Combined sources15
Helixi205 – 207Combined sources3
Beta strandi209 – 216Combined sources8
Beta strandi219 – 237Combined sources19
Turni238 – 241Combined sources4
Beta strandi242 – 249Combined sources8
Helixi250 – 252Combined sources3
Beta strandi253 – 263Combined sources11
Beta strandi266 – 270Combined sources5
Helixi272 – 276Combined sources5
Helixi280 – 287Combined sources8
Beta strandi293 – 302Combined sources10
Beta strandi304 – 311Combined sources8
Helixi313 – 319Combined sources7
Helixi323 – 325Combined sources3
Turni332 – 334Combined sources3
Beta strandi336 – 338Combined sources3
Beta strandi341 – 353Combined sources13
Beta strandi355 – 369Combined sources15
Beta strandi379 – 382Combined sources4
Beta strandi387 – 393Combined sources7
Turni394 – 397Combined sources4
Beta strandi398 – 405Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DUTX-ray3.00A/B/C/D1-410[»]
2H4PX-ray1.70A1-369[»]
B377-410[»]
2H4QX-ray2.10A1-369[»]
B377-410[»]
2H4RX-ray2.70A1-410[»]
ProteinModelPortaliO73790.
SMRiO73790.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO73790.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni61 – 69M-loop required for interaction with nucleosome linker DNA9
Regioni356 – 372Required for oligomerization and DNA condensationAdd BLAST17

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi80 – 84Nuclear localization signalBy similarity5

Sequence similaritiesi

Belongs to the serpin family. Ov-serpin subfamily.Curated

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
GeneTreeiENSGT00760000118789.
HOGENOMiHOG000238519.
HOVERGENiHBG005957.
InParanoidiO73790.
KOiK13963.
OMAiRMDPEHE.
OrthoDBiEOG091G0ION.
PhylomeDBiO73790.
TreeFamiTF352619.

Family and domain databases

InterProiIPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 2 hits.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 2 hits.

Sequencei

Sequence statusi: Complete.

O73790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQVSASIGN FTVDLFNKLN ETNRDKNIFF SPWSISSALA LTYLAAKGST
60 70 80 90 100
AREMAEVLHF TEAVRAESSS VARPSRGRPK RRRMDPEHEQ AENIHSGFKE
110 120 130 140 150
LLTAFNKPRN NYSLRSANRI YVEKTYALLP TYLQLSKKYY KAEPQKVNFK
160 170 180 190 200
TAPEQSRKEI NTWVEKQTES KIKNLLSSDD VKATTRLILV NAIYFKAEWE
210 220 230 240 250
VKFQAEKTSI QPFRLSKNKS KPVKMMYMRD TFPVLIMEKM NFKMIELPYV
260 270 280 290 300
KRELSMFILL PDDIKDGTTG LEQLERELTY ERLSEWADSK MMTETLVDLH
310 320 330 340 350
LPKFSLEDRI DLRDTLRNMG MTTAFTTNAD FRGMTDKKDL AISKVIHQSF
360 370 380 390 400
VAVDEKGTEA AAATAVIISF TTSVINHVLK FKVDHPFHFF IRHNKSKTIL
410
FFGRFCCPVE
Length:410
Mass (Da):47,384
Last modified:August 1, 1998 - v1
Checksum:i24FC06D9C4F4F0BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053401 mRNA. Translation: AAC15710.1.
RefSeqiNP_990228.1. NM_204897.1.
XP_015137649.1. XM_015282163.1.
XP_015137651.1. XM_015282165.1.
UniGeneiGga.415.

Genome annotation databases

EnsembliENSGALT00000020990; ENSGALP00000020960; ENSGALG00000019553.
GeneIDi395715.
KEGGigga:395715.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053401 mRNA. Translation: AAC15710.1.
RefSeqiNP_990228.1. NM_204897.1.
XP_015137649.1. XM_015282163.1.
XP_015137651.1. XM_015282165.1.
UniGeneiGga.415.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DUTX-ray3.00A/B/C/D1-410[»]
2H4PX-ray1.70A1-369[»]
B377-410[»]
2H4QX-ray2.10A1-369[»]
B377-410[»]
2H4RX-ray2.70A1-410[»]
ProteinModelPortaliO73790.
SMRiO73790.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000020960.

Protein family/group databases

MEROPSiI04.033.

Proteomic databases

PaxDbiO73790.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000020990; ENSGALP00000020960; ENSGALG00000019553.
GeneIDi395715.
KEGGigga:395715.

Organism-specific databases

CTDi5273.

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
GeneTreeiENSGT00760000118789.
HOGENOMiHOG000238519.
HOVERGENiHBG005957.
InParanoidiO73790.
KOiK13963.
OMAiRMDPEHE.
OrthoDBiEOG091G0ION.
PhylomeDBiO73790.
TreeFamiTF352619.

Enzyme and pathway databases

ReactomeiR-GGA-6798695. Neutrophil degranulation.

Miscellaneous databases

EvolutionaryTraceiO73790.
PROiO73790.

Gene expression databases

BgeeiENSGALG00000019553.

Family and domain databases

InterProiIPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 2 hits.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiSPB10_CHICK
AccessioniPrimary (citable) accession number: O73790
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.