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Protein

Guanylyl cyclase-activating protein 1

Gene

GUCA1A

Organism
Lithobates pipiens (Northern leopard frog) (Rana pipiens)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Regulatory protein that inhibits guanylyl cyclase when free calcium ions concentration is elevated. This Ca2+-sensitive regulation of retinal guanylyl cyclase is a key event in recovery of the dark state of rod photoreceptors following light exposure.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi63 – 74121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi99 – 110122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi142 – 153123PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylyl cyclase-activating protein 1
Short name:
GCAP 1
Alternative name(s):
Guanylate cyclase activator 1A
Gene namesi
Name:GUCA1A
Synonyms:GCAP1
OrganismiLithobates pipiens (Northern leopard frog) (Rana pipiens)
Taxonomic identifieri8404 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraNeobatrachiaRanoideaRanidaeRana

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedSequence analysis
Chaini2 – 205204Guanylyl cyclase-activating protein 1PRO_0000073806Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Modified residuei3 – 31Deamidated asparagineSequence analysis

Keywords - PTMi

Lipoprotein, Myristate

Expressioni

Tissue specificityi

Retina.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO73761.
SMRiO73761. Positions 2-185.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 4836EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini50 – 8536EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini86 – 12136EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini129 – 16436EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Domaini

Binds three calcium ions (via EF-hands 2, 3 and 4) when calcium levels are high. Binds Mg2+ when calcium levels are low.By similarity

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG108179.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR033022. GUCA1A.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PTHR23055:SF13. PTHR23055:SF13. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O73761-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNMDGKTVE ELSATEIHRW YKKFMTECPS GQLTQHEFKQ FFGLKNLSPA
60 70 80 90 100
SNQYIEQMFD TFDFNKDGYM DFMEYVAALS LVLKGKVEQK LRWYFKLYDV
110 120 130 140 150
DGNGCIDRGE LLNIIKAIRA INRCNDEMTA EEFTDMVFDK IDINGDGELS
160 170 180 190 200
LEEFIEGVQK DELLLEVLTR SLDLKHIVYM IQNDGKRMEI SERPRQEITT

GNSLP
Length:205
Mass (Da):23,840
Last modified:January 23, 2007 - v3
Checksum:i00C6564F0D3434BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047882 mRNA. Translation: AAC15876.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047882 mRNA. Translation: AAC15876.1.

3D structure databases

ProteinModelPortaliO73761.
SMRiO73761. Positions 2-185.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG108179.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR033022. GUCA1A.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PTHR23055:SF13. PTHR23055:SF13. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Guanylate-cyclase-inhibitory protein is a frog retinal Ca2+-binding protein related to mammalian guanylate-cyclase-activating proteins."
    Li N., Fariss R.N., Zhang K., Otto-Bruc A.E., Haeseleer F., Bronson J.D., Qin N., Yamazaki A., Subbaraya I., Milam A.H., Palczewski K., Baehr W.
    Eur. J. Biochem. 252:591-599(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Retina.

Entry informationi

Entry nameiGUC1A_LITPI
AccessioniPrimary (citable) accession number: O73761
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 80 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.