O73727 (INS_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin

Cleaved into the following 2 chains:

1. Insulin B chain
2. Insulin A chain

Gene names

Name:

ins

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	108 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Subunit structure	Heterodimer of a B chain and an A chain linked by two disulfide bonds.
Subcellular location	Secreted.
Sequence similarities	Belongs to the insulin family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	By similarity
	Peptide	22 – 51	30	Insulin B chain		PRO_0000015767
	Propeptide	54 – 84	31	C peptide		PRO_0000015768
	Peptide	88 – 108	21	Insulin A chain		PRO_0000015769
Amino acid modifications
	Disulfide bond	30 ↔ 94		Interchain (between B and A chains) By similarity
	Disulfide bond	42 ↔ 107		Interchain (between B and A chains) By similarity
	Disulfide bond	93 ↔ 98		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O73727 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 31D03CCF37BD2C22	FASTA	108	11,904
	10 20 30 40 50 60 MAVWLQAGAL LVLLVVSSVS TNPGTPQHLC GSHLVDALYL VCGPTGFFYN PKRDVEPLLG 70 80 90 100 FLPPKSAQET EVADFAFKDH AELIRKRGIV EQCCHKPCSI FELQNYCN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Conservation of PDX-1 structure, function, and expression in zebrafish." Milewski W.M., Duguay S.J., Chan S.J., Steiner D.F. Endocrinology 139:1440-1449(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF036326 mRNA. Translation: AAC41261.1. BC095013 mRNA. Translation: AAH95013.1.
IPI	IPI00507062.
RefSeq	NP_571131.1. NM_131056.1.
UniGene	Dr.75811.
3D structure databases
ProteinModelPortal	O73727.
SMR	O73727. Positions 27-52.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000051222; ENSDARP00000051221; ENSDARG00000035350.
GeneID	30262.
KEGG	dre:30262.
Organism-specific databases
CTD	3630.
ZFIN	ZDB-GENE-980526-110. ins.
Phylogenomic databases
eggNOG	NOG45999.
GeneTree	ENSGT00390000015440.
HOGENOM	HOG000261669.
HOVERGEN	HBG006137.
InParanoid	O73727.
KO	K04526.
OMA	AMAPPQH.
OrthoDB	EOG4RNB9Q.
Gene expression databases
ArrayExpress	O73727.
Bgee	O73727.
Family and domain databases
Gene3D	1.10.100.10. 2 hits.
InterPro	IPR004825. Insulin. IPR016179. Insulin-like. IPR022353. Insulin_CS. IPR022352. Insulin_family. [Graphical view]
Pfam	PF00049. Insulin. 1 hit. [Graphical view]
PRINTS	PR00277. INSULIN. PR00276. INSULINFAMLY.
SMART	SM00078. IlGF. 1 hit. [Graphical view]
SUPFAM	SSF56994. Insulin-like. 1 hit.
PROSITE	PS00262. INSULIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20806709.

Entry information

Entry name

INS_DANRE

Accession

Primary (citable) accession number: O73727
Secondary accession number(s): Q504H9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 1, 1998
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program