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Protein

Insulin

Gene

ins

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

GO - Biological processi

  • glucose metabolic process Source: UniProtKB-KW
  • negative regulation of feeding behavior Source: ZFIN
  • response to glucose Source: ZFIN
  • response to nutrient levels Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Hormone

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Enzyme and pathway databases

ReactomeiR-DRE-264876. Insulin processing.
R-DRE-6807878. COPI-mediated anterograde transport.
R-DRE-74713. IRS activation.
R-DRE-74749. Signal attenuation.
R-DRE-74751. Insulin receptor signalling cascade.
R-DRE-74752. Signaling by Insulin receptor.
R-DRE-77387. Insulin receptor recycling.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin
Cleaved into the following 2 chains:
Gene namesi
Name:ins
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 5

Organism-specific databases

ZFINiZDB-GENE-980526-110. ins.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121By similarityAdd
BLAST
Peptidei22 – 5130Insulin B chainPRO_0000015767Add
BLAST
Propeptidei54 – 8431C peptidePRO_0000015768Add
BLAST
Peptidei88 – 10821Insulin A chainPRO_0000015769Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 94Interchain (between B and A chains)By similarity
Disulfide bondi42 ↔ 107Interchain (between B and A chains)By similarity
Disulfide bondi93 ↔ 98By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbiO73727.

Expressioni

Gene expression databases

BgeeiO73727.
ExpressionAtlasiO73727. differential.

Interactioni

Subunit structurei

Heterodimer of a B chain and an A chain linked by two disulfide bonds.

Protein-protein interaction databases

STRINGi7955.ENSDARP00000051221.

Structurei

3D structure databases

ProteinModelPortaliO73727.
SMRiO73727. Positions 27-52.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the insulin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J0XC. Eukaryota.
ENOG4111VJB. LUCA.
GeneTreeiENSGT00390000015440.
HOGENOMiHOG000261669.
HOVERGENiHBG006137.
InParanoidiO73727.
KOiK04526.
OMAiGFFYSPK.
OrthoDBiEOG7TF7CG.
TreeFamiTF332820.

Family and domain databases

Gene3Di1.10.100.10. 2 hits.
InterProiIPR004825. Insulin.
IPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR00277. INSULIN.
PR00276. INSULINFAMLY.
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O73727-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVWLQAGAL LVLLVVSSVS TNPGTPQHLC GSHLVDALYL VCGPTGFFYN
60 70 80 90 100
PKRDVEPLLG FLPPKSAQET EVADFAFKDH AELIRKRGIV EQCCHKPCSI

FELQNYCN
Length:108
Mass (Da):11,904
Last modified:August 1, 1998 - v1
Checksum:i31D03CCF37BD2C22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036326 mRNA. Translation: AAC41261.1.
BC095013 mRNA. Translation: AAH95013.1.
RefSeqiNP_571131.1. NM_131056.1.
UniGeneiDr.75811.

Genome annotation databases

EnsembliENSDART00000051222; ENSDARP00000051221; ENSDARG00000035350.
GeneIDi30262.
KEGGidre:30262.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036326 mRNA. Translation: AAC41261.1.
BC095013 mRNA. Translation: AAH95013.1.
RefSeqiNP_571131.1. NM_131056.1.
UniGeneiDr.75811.

3D structure databases

ProteinModelPortaliO73727.
SMRiO73727. Positions 27-52.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000051221.

Proteomic databases

PaxDbiO73727.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000051222; ENSDARP00000051221; ENSDARG00000035350.
GeneIDi30262.
KEGGidre:30262.

Organism-specific databases

CTDi3630.
ZFINiZDB-GENE-980526-110. ins.

Phylogenomic databases

eggNOGiENOG410J0XC. Eukaryota.
ENOG4111VJB. LUCA.
GeneTreeiENSGT00390000015440.
HOGENOMiHOG000261669.
HOVERGENiHBG006137.
InParanoidiO73727.
KOiK04526.
OMAiGFFYSPK.
OrthoDBiEOG7TF7CG.
TreeFamiTF332820.

Enzyme and pathway databases

ReactomeiR-DRE-264876. Insulin processing.
R-DRE-6807878. COPI-mediated anterograde transport.
R-DRE-74713. IRS activation.
R-DRE-74749. Signal attenuation.
R-DRE-74751. Insulin receptor signalling cascade.
R-DRE-74752. Signaling by Insulin receptor.
R-DRE-77387. Insulin receptor recycling.

Miscellaneous databases

PROiO73727.

Gene expression databases

BgeeiO73727.
ExpressionAtlasiO73727. differential.

Family and domain databases

Gene3Di1.10.100.10. 2 hits.
InterProiIPR004825. Insulin.
IPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR00277. INSULIN.
PR00276. INSULINFAMLY.
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Conservation of PDX-1 structure, function, and expression in zebrafish."
    Milewski W.M., Duguay S.J., Chan S.J., Steiner D.F.
    Endocrinology 139:1440-1449(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiINS_DANRE
AccessioniPrimary (citable) accession number: O73727
Secondary accession number(s): Q504H9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: June 8, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.