O73686 (CP192_CARAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 19 type 2
EC=1.14.14.1

Alternative name(s):
CYPXIXA2
Cytochrome P-450AROM
Estrogen synthase
Ovarian aromatase

Gene names

Name:

cyp19a2

Organism

Carassius auratus (Goldfish)

Taxonomic identifier

7957 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Carassius

Protein attributes

Sequence length	518 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Membrane; Peripheral membrane protein.
Tissue specificity	Ovarian specific.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Membrane
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 518	518	Cytochrome P450 19 type 2		PRO_0000051969
Sites
	Metal binding	457	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	85	1	F → L in BAA23758. Ref.2
	Sequence conflict	166	1	S → T in BAA23758. Ref.2
	Sequence conflict	263	1	R → G in BAA23758. Ref.2
	Sequence conflict	300	1	A → G in BAA23758. Ref.2
	Sequence conflict	373	1	F → L in BAA23758. Ref.2
	Sequence conflict	407	1	K → E in BAA23758. Ref.2
	Sequence conflict	410	1	R → K in BAA23758. Ref.2
	Sequence conflict	462	1	M → I in BAA23758. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O73686 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 004A9333D8952DEB	FASTA	518	58,612
	10 20 30 40 50 60 MAGELLQPCG MKQVHLGEAV LELLMQGAHN SSYGAQDNVC GAMATLLLLL LCLLLAIRHH 70 80 90 100 110 120 WTEKDHVPGP CFLLGLGPLL SYCRFIWSGI GTASNYYNSK YGDIVRVWIN GEETLILSRS 130 140 150 160 170 180 SAVYHVLRKS LYTSRFGSKL GLQCIGMHEQ GIIFNSNVAL WKKVRSFYAK ALTGPGLQRT 190 200 210 220 230 240 LEICITSTNT HLDNLSHLMD ARGQVDILNL LRCIVVDISN RLFLGVPLNE HDLLQKIHKY 250 260 270 280 290 300 FDTWQTVLIK PDVYFRLAWW LHRKHKRDAQ ELQDAIAALI EQKRVQLTRA EKFDQLDFTA 310 320 330 340 350 360 ELIFAQSHGE LSTENVRQCV LEMIIAAPDT LSISLFFMLL LLKQNPDVEL KILQEMNAVL 370 380 390 400 410 420 AGRSLQHSHL SGFHILESFI NESLRFHPVV DFTMRRALDD DVIEGYKVKR GTNIILNVGR 430 440 450 460 470 480 MHRSEFFPKP NEFSLDNFQK NVPSRFFQPF GSGPRSCVGK HMAMVMMKSI LVTLLSRFSV 490 500 510 CPVKGCTVDS IPQTNDLSQQ PVEEPSSLSV QLILRNAL

References

[1]	"Identification of multiple CYP19 genes encoding different cytochrome P450 aromatase isozymes in brain and ovary." Tchoudakova A., Callard G.V. Endocrinology 139:2179-2189(1998) [PubMed: 9529008] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.
[2]	"Two differing cDNAs encoding cytochrome P450arom (aromatase) exist in goldfish." Sudrajat A., Yoshiura Y., Gen K., Suetake H., Aida K. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF020704 mRNA. Translation: AAC14013.1. AB009336 mRNA. Translation: BAA23758.1.
3D structure databases
ProteinModelPortal	O73686.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG050750.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP192_CARAU

Accession

Primary (citable) accession number: O73686
Secondary accession number(s): O57316

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	September 21, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program