O73683 (A4_TETFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Amyloid beta A4 protein

Alternative name(s):
ABPP
Short name=APP
Alzheimer disease amyloid A4 protein homolog

Cleaved into the following chain:

1. Beta-amyloid protein
   Alternative name(s):
   A-beta
   Beta-APP

Gene names

Name:

app

Organism

Tetraodon fluviatilis (Green pufferfish) (Chelonodon fluviatilis)

Taxonomic identifier

47145 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Euteleostei › Neognathi › Neoteleostei › Eurypterygii › Ctenosquamata › Acanthomorpha › Euacanthomorpha › Holacanthopterygii › Acanthopterygii › Euacanthopterygii › Percomorpha › Tetraodontiformes › Tetraodontoidei › Tetradontoidea › Tetraodontidae › Tetraodon

Protein attributes

Sequence length	780 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Functional neuronal receptor which couples to intracellular signaling pathway through the GTP-binding protein G(O) By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the APP family. Contains 1 BPTI/Kunitz inhibitor domain.

Ontologies

Keywords
Cellular component	Amyloid Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	Copper Metal-binding
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	heparin binding Inferred from electronic annotation. Source: InterPro serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 780	762	Amyloid beta A4 protein		PRO_0000000199
	Chain	682 – 724	43	Beta-amyloid protein Potential		PRO_0000000200
Regions
	Topological domain	19 – 711	693	Extracellular Potential
	Transmembrane	712 – 732	21	Helical; Potential
	Topological domain	733 – 780	48	Cytoplasmic Potential
	Domain	323 – 382	60	BPTI/Kunitz inhibitor
	Region	769 – 772	4	Clathrin-binding By similarity
Sites
	Metal binding	148	1	Copper By similarity
	Metal binding	152	1	Copper By similarity
	Metal binding	169	1	Copper By similarity
Amino acid modifications
	Glycosylation	560	1	N-linked (GlcNAc...) Potential
	Disulfide bond	39 ↔ 63		By similarity
	Disulfide bond	74 ↔ 118		By similarity
	Disulfide bond	99 ↔ 106		By similarity
	Disulfide bond	134 ↔ 188		By similarity
	Disulfide bond	145 ↔ 175		By similarity
	Disulfide bond	159 ↔ 187		By similarity
	Disulfide bond	327 ↔ 378		By similarity
	Disulfide bond	336 ↔ 361		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O73683 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 60071BE94520191D	FASTA	780	88,238
	10 20 30 40 50 60 MGHSVAWLLL VAAASTLAAE VPTDVSMGLL AEPQVAMFCG KINMHINVQS GKWEPDPSGT 70 80 90 100 110 120 KSCIGTKEGI LQYCQEVYPE LQITNVVEAN QPVSIQNWCK KGRKQCRSHM HIVVPYRCLV 130 140 150 160 170 180 GEFVSDALLV PDKCKFLHQE RMNQCESHLH WHTVAKESCG DRAMNLHDYG MLLPCGIDRF 190 200 210 220 230 240 RGVEFVCCPA EAERDMDSTE KDADDSDVWW GGADNDYSDN SMVREPEPAE QQEETRPSVV 250 260 270 280 290 300 EEEEEGEVAQ EDDEEEEEVL DTDQDGDGEE DHEAADDEEE EEDVDEIDAF GESDDVDADE 310 320 330 340 350 360 PTTNVAMTTT TTTTTTESVE EVVRMFCWAH ADTGPCTASM PSWYFDAVDG RTMYELMYGG 370 380 390 400 410 420 CGGNMNNFES EEYCLSVCSS VVPTDMPSSP DAVDHYLETP ADENEHAHFQ KAKESLEAKH 430 440 450 460 470 480 RERMSQVMRE WEEAERQAKN LPRADKKIVI QRFQEKVEAL EQEAASERQQ LVETHMARVE 490 500 510 520 530 540 ALLNDRRRLA LENYLTALQQ DPPRPRHVFS LLKKYVRAEQ KDRQHTLKHF EHVRMVDPKK 550 560 570 580 590 600 AAQIRPQVLT HLRVIEERMN QSLGLLYKVP GVADDIQDQV ELLQREQAEM AQQLANLQTD 610 620 630 640 650 660 VRVSYGNDAL MPDQELGDGQ ADLLPQEDTL GGVGFVHPES FNQLNTENQV EPVDSRPTFE 670 680 690 700 710 720 RGVPTRPVTG KSMEAVPELR METEDRQSTE YEVHHQKLVF FAEDVGSNKG AIIGLMVGGV 730 740 750 760 770 780 VIATVIVITL VMLRKKQYTS IHHGIIEVDA AVTPEERHLS KMQQNGYENP TYKFFEQMQN

References

[1]	"Analysis of pufferfish homologues of the AT-rich human APP gene." Villard L., Tassone F., Crnogorac-Jurcevic T., Clancy K., Gardiner K. Gene 210:17-24(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF018165 mRNA. Translation: AAC41275.1.
3D structure databases
ProteinModelPortal	O73683.
SMR	O73683. Positions 29-124, 392-583, 691-716, 749-780.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG000051.
Family and domain databases
Gene3D	3.30.1490.140. 1 hit. 3.90.570.10. 1 hit. 4.10.230.10. 1 hit. 4.10.410.10. 1 hit.
InterPro	IPR008155. Amyloid_glyco. IPR013803. Amyloid_glyco_Abeta. IPR011178. Amyloid_glyco_Cu-bd. IPR024329. Amyloid_glyco_E2_domain. IPR008154. Amyloid_glyco_extra. IPR019744. Amyloid_glyco_extracell_CS. IPR015849. Amyloid_glyco_heparin-bd. IPR019745. Amyloid_glyco_intracell_CS. IPR019543. APP_amyloid_C. IPR002223. Prot_inh_Kunz-m. [Graphical view]
Pfam	PF10515. APP_amyloid. 1 hit. PF12924. APP_Cu_bd. 1 hit. PF12925. APP_E2. 1 hit. PF02177. APP_N. 1 hit. PF03494. Beta-APP. 1 hit. PF00014. Kunitz_BPTI. 1 hit. [Graphical view]
PRINTS	PR00203. AMYLOIDA4. PR00759. BASICPTASE. PR00204. BETAAMYLOID.
SMART	SM00006. A4_EXTRA. 1 hit. SM00131. KU. 1 hit. [Graphical view]
SUPFAM	SSF56491. A4_extra. 1 hit. SSF89811. Amyloid_glyco_Cu-bd. 1 hit. SSF57362. Prot_inh_Kunz-m. 1 hit. SSF109843. SSF109843. 1 hit.
PROSITE	PS00319. A4_EXTRA. 1 hit. PS00320. A4_INTRA. 1 hit. PS00280. BPTI_KUNITZ_1. False negative. PS50279. BPTI_KUNITZ_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A4_TETFL

Accession

Primary (citable) accession number: O73683

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	August 1, 1998
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program